Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression
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Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression. / Bomholt, Anna Billeschou; Johansen, Christian Dall; Christensen, Jens Bager; Kjeldsen, Sasha Alexandra Sampson; Galsgaard, Katrine Douglas; Winther-Sørensen, Marie; Serizawa, Reza; Hornum, Mads; Porrini, Esteban; Pedersen, Jens; Ørskov, Cathrine; Gluud, Lise Lotte; Sørensen, Charlotte Mehlin; Holst, Jens Juul; Albrechtsen, Reidar; Wewer Albrechtsen, Nicolai Jacob.
In: Communications Biology , Vol. 5, No. 1, 1278, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression
AU - Bomholt, Anna Billeschou
AU - Johansen, Christian Dall
AU - Christensen, Jens Bager
AU - Kjeldsen, Sasha Alexandra Sampson
AU - Galsgaard, Katrine Douglas
AU - Winther-Sørensen, Marie
AU - Serizawa, Reza
AU - Hornum, Mads
AU - Porrini, Esteban
AU - Pedersen, Jens
AU - Ørskov, Cathrine
AU - Gluud, Lise Lotte
AU - Sørensen, Charlotte Mehlin
AU - Holst, Jens Juul
AU - Albrechtsen, Reidar
AU - Wewer Albrechtsen, Nicolai Jacob
N1 - © 2022. The Author(s).
PY - 2022
Y1 - 2022
N2 - Glucagon is a major regulator of metabolism and drugs targeting the glucagon receptor (GCGR) are being developed. Insight into tissue and cell-specific expression of the GCGR is important to understand the biology of glucagon and to differentiate between direct and indirect actions of glucagon. However, it has been challenging to localize the GCGR in tissue due to low expression levels and lack of specific methods. Immunohistochemistry has frequently been used for GCGR localization, but antibodies targeting G-protein-coupled-receptors may be inaccurate. We evaluated all currently commercially available GCGR antibodies. The antibody, ab75240 (Antibody no. 11) was found to perform best among the twelve antibodies tested and using this antibody we found expression of the GCGR in the kidney, liver, preadipocytes, pancreas, and heart. Three antibody-independent approaches all confirmed the presence of the GCGR within the pancreas, liver and the kidneys. GCGR expression should be evaluated by both antibody and antibody-independent approaches.
AB - Glucagon is a major regulator of metabolism and drugs targeting the glucagon receptor (GCGR) are being developed. Insight into tissue and cell-specific expression of the GCGR is important to understand the biology of glucagon and to differentiate between direct and indirect actions of glucagon. However, it has been challenging to localize the GCGR in tissue due to low expression levels and lack of specific methods. Immunohistochemistry has frequently been used for GCGR localization, but antibodies targeting G-protein-coupled-receptors may be inaccurate. We evaluated all currently commercially available GCGR antibodies. The antibody, ab75240 (Antibody no. 11) was found to perform best among the twelve antibodies tested and using this antibody we found expression of the GCGR in the kidney, liver, preadipocytes, pancreas, and heart. Three antibody-independent approaches all confirmed the presence of the GCGR within the pancreas, liver and the kidneys. GCGR expression should be evaluated by both antibody and antibody-independent approaches.
KW - Receptors, Glucagon/genetics
KW - Glucagon
KW - Gene Expression
KW - Antibodies/metabolism
KW - Liver/metabolism
U2 - 10.1038/s42003-022-04242-7
DO - 10.1038/s42003-022-04242-7
M3 - Journal article
C2 - 36418521
VL - 5
JO - Communications Biology
JF - Communications Biology
SN - 2399-3642
IS - 1
M1 - 1278
ER -
ID: 327122328