Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men

Research output: Contribution to journalJournal articlepeer-review

Standard

Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men. / Vigelsø Hansen, Andreas; Gram, Martin; Wiuff, Caroline; Hansen, Christina Neigaard; Prats Gavalda, Clara; Dela, Flemming; Helge, Jørn Wulff.

In: European Journal of Applied Physiology, Vol. 116, No. 3, 03.2016, p. 481-494.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Vigelsø Hansen, A, Gram, M, Wiuff, C, Hansen, CN, Prats Gavalda, C, Dela, F & Helge, JW 2016, 'Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men', European Journal of Applied Physiology, vol. 116, no. 3, pp. 481-494. https://doi.org/10.1007/s00421-015-3302-x

APA

Vigelsø Hansen, A., Gram, M., Wiuff, C., Hansen, C. N., Prats Gavalda, C., Dela, F., & Helge, J. W. (2016). Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men. European Journal of Applied Physiology, 116(3), 481-494. https://doi.org/10.1007/s00421-015-3302-x

Vancouver

Vigelsø Hansen A, Gram M, Wiuff C, Hansen CN, Prats Gavalda C, Dela F et al. Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men. European Journal of Applied Physiology. 2016 Mar;116(3):481-494. https://doi.org/10.1007/s00421-015-3302-x

Author

Vigelsø Hansen, Andreas ; Gram, Martin ; Wiuff, Caroline ; Hansen, Christina Neigaard ; Prats Gavalda, Clara ; Dela, Flemming ; Helge, Jørn Wulff. / Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men. In: European Journal of Applied Physiology. 2016 ; Vol. 116, No. 3. pp. 481-494.

Bibtex

@article{d6b3ab2b058842249107799791d8cd8e,
title = "Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men",
abstract = "PURPOSE: Aging and inactivity lead to skeletal muscle metabolic inflexibility, but the underlying molecular mechanisms are not entirely elucidated. Therefore, we investigated how muscle lipid and glycogen stores and major regulatory proteins were affected by short-term immobilization followed by aerobic training in young and older men.METHODS: 17 young (23 ± 1 years, 24 ± 1 kg m(-2), and 20 ± 2% body fat) and 15 older men (68 ± 1 years; 27 ± 1 kg m(-2), and 29 ± 2% body fat) underwent 2 weeks' one leg immobilization followed by 6 weeks' cycle training. Biopsies were obtained from m. vastus lateralis just before immobilization (at inclusion), after immobilization, and the after 6 weeks' training. The biopsies were analyzed for muscle substrates; muscle perilipin protein (PLIN), glycogen synthase (GS), synaptosomal-associated protein of 23 kDa (SNAP23) protein content, and muscle 3-hydroxyacyl-CoA dehydrogenase (HAD) activityRESULTS: The older men had higher intramuscular triglyceride (IMTG) (73 %) and Glycogen (16%) levels compared to the young men, and IMTG tended to increase with immobilization. PLIN2 and 3 protein content increased with immobilization in the older men only. The young men had higher GS (74%) protein compared to the older men. Immobilization decreased and training restored HAD activity, GS and SNAP23 protein content in young and older men.CONCLUSION: Evidence of age-related metabolic inflexibility is presented, seen as body fat and IMTG accumulation. The question arises as to whether IMTG accumulation in the older men is caused by or leading to the increase in PLIN2 and 3 protein content. Training decreased body fat and IMTG levels in both young and older men; hence, training should be prioritized to reduce the detrimental effect of aging on metabolism.",
author = "{Vigels{\o} Hansen}, Andreas and Martin Gram and Caroline Wiuff and Hansen, {Christina Neigaard} and {Prats Gavalda}, Clara and Flemming Dela and Helge, {J{\o}rn Wulff}",
year = "2016",
month = mar,
doi = "10.1007/s00421-015-3302-x",
language = "English",
volume = "116",
pages = "481--494",
journal = "European Journal of Applied Physiology",
issn = "1439-6319",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men

AU - Vigelsø Hansen, Andreas

AU - Gram, Martin

AU - Wiuff, Caroline

AU - Hansen, Christina Neigaard

AU - Prats Gavalda, Clara

AU - Dela, Flemming

AU - Helge, Jørn Wulff

PY - 2016/3

Y1 - 2016/3

N2 - PURPOSE: Aging and inactivity lead to skeletal muscle metabolic inflexibility, but the underlying molecular mechanisms are not entirely elucidated. Therefore, we investigated how muscle lipid and glycogen stores and major regulatory proteins were affected by short-term immobilization followed by aerobic training in young and older men.METHODS: 17 young (23 ± 1 years, 24 ± 1 kg m(-2), and 20 ± 2% body fat) and 15 older men (68 ± 1 years; 27 ± 1 kg m(-2), and 29 ± 2% body fat) underwent 2 weeks' one leg immobilization followed by 6 weeks' cycle training. Biopsies were obtained from m. vastus lateralis just before immobilization (at inclusion), after immobilization, and the after 6 weeks' training. The biopsies were analyzed for muscle substrates; muscle perilipin protein (PLIN), glycogen synthase (GS), synaptosomal-associated protein of 23 kDa (SNAP23) protein content, and muscle 3-hydroxyacyl-CoA dehydrogenase (HAD) activityRESULTS: The older men had higher intramuscular triglyceride (IMTG) (73 %) and Glycogen (16%) levels compared to the young men, and IMTG tended to increase with immobilization. PLIN2 and 3 protein content increased with immobilization in the older men only. The young men had higher GS (74%) protein compared to the older men. Immobilization decreased and training restored HAD activity, GS and SNAP23 protein content in young and older men.CONCLUSION: Evidence of age-related metabolic inflexibility is presented, seen as body fat and IMTG accumulation. The question arises as to whether IMTG accumulation in the older men is caused by or leading to the increase in PLIN2 and 3 protein content. Training decreased body fat and IMTG levels in both young and older men; hence, training should be prioritized to reduce the detrimental effect of aging on metabolism.

AB - PURPOSE: Aging and inactivity lead to skeletal muscle metabolic inflexibility, but the underlying molecular mechanisms are not entirely elucidated. Therefore, we investigated how muscle lipid and glycogen stores and major regulatory proteins were affected by short-term immobilization followed by aerobic training in young and older men.METHODS: 17 young (23 ± 1 years, 24 ± 1 kg m(-2), and 20 ± 2% body fat) and 15 older men (68 ± 1 years; 27 ± 1 kg m(-2), and 29 ± 2% body fat) underwent 2 weeks' one leg immobilization followed by 6 weeks' cycle training. Biopsies were obtained from m. vastus lateralis just before immobilization (at inclusion), after immobilization, and the after 6 weeks' training. The biopsies were analyzed for muscle substrates; muscle perilipin protein (PLIN), glycogen synthase (GS), synaptosomal-associated protein of 23 kDa (SNAP23) protein content, and muscle 3-hydroxyacyl-CoA dehydrogenase (HAD) activityRESULTS: The older men had higher intramuscular triglyceride (IMTG) (73 %) and Glycogen (16%) levels compared to the young men, and IMTG tended to increase with immobilization. PLIN2 and 3 protein content increased with immobilization in the older men only. The young men had higher GS (74%) protein compared to the older men. Immobilization decreased and training restored HAD activity, GS and SNAP23 protein content in young and older men.CONCLUSION: Evidence of age-related metabolic inflexibility is presented, seen as body fat and IMTG accumulation. The question arises as to whether IMTG accumulation in the older men is caused by or leading to the increase in PLIN2 and 3 protein content. Training decreased body fat and IMTG levels in both young and older men; hence, training should be prioritized to reduce the detrimental effect of aging on metabolism.

U2 - 10.1007/s00421-015-3302-x

DO - 10.1007/s00421-015-3302-x

M3 - Journal article

C2 - 26626913

VL - 116

SP - 481

EP - 494

JO - European Journal of Applied Physiology

JF - European Journal of Applied Physiology

SN - 1439-6319

IS - 3

ER -

ID: 167806138