Effect of solvent composition on the extraction of proteins from hemp oil processing stream

Research output: Contribution to journalJournal articlepeer-review

Standard

Effect of solvent composition on the extraction of proteins from hemp oil processing stream. / Cabral, Eduarda M.; Poojary, Mahesha M.; Lund, Marianne N.; Curtin, James; Fenelon, Mark; Tiwari, Brijesh K.

In: Journal of the Science of Food and Agriculture, Vol. 102, No. 14, 2022, p. 6293-6298.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Cabral, EM, Poojary, MM, Lund, MN, Curtin, J, Fenelon, M & Tiwari, BK 2022, 'Effect of solvent composition on the extraction of proteins from hemp oil processing stream', Journal of the Science of Food and Agriculture, vol. 102, no. 14, pp. 6293-6298. https://doi.org/10.1002/jsfa.11979

APA

Cabral, E. M., Poojary, M. M., Lund, M. N., Curtin, J., Fenelon, M., & Tiwari, B. K. (2022). Effect of solvent composition on the extraction of proteins from hemp oil processing stream. Journal of the Science of Food and Agriculture, 102(14), 6293-6298. https://doi.org/10.1002/jsfa.11979

Vancouver

Cabral EM, Poojary MM, Lund MN, Curtin J, Fenelon M, Tiwari BK. Effect of solvent composition on the extraction of proteins from hemp oil processing stream. Journal of the Science of Food and Agriculture. 2022;102(14):6293-6298. https://doi.org/10.1002/jsfa.11979

Author

Cabral, Eduarda M. ; Poojary, Mahesha M. ; Lund, Marianne N. ; Curtin, James ; Fenelon, Mark ; Tiwari, Brijesh K. / Effect of solvent composition on the extraction of proteins from hemp oil processing stream. In: Journal of the Science of Food and Agriculture. 2022 ; Vol. 102, No. 14. pp. 6293-6298.

Bibtex

@article{6876eacfb13f4bfd8087b3dc271a994f,
title = "Effect of solvent composition on the extraction of proteins from hemp oil processing stream",
abstract = "BACKGROUND: Hempseed meal, a by-product of the hempseed oil processing stream, is a potential alternative source for food proteins. Efficient extraction of proteins from hempseed meal is challenging owing to differences in the structure and solubility of various protein fractions present in the seed. In the present study, protein was extracted from hempseed meal using four different solvents, including aqueous NaOH, KOH, NaHCO3 and NaCl, at four different concentrations with the aim of improving the recovery of protein fractions rich in essential amino acids. RESULTS: Extraction using alkaline solvents provided superior protein recovery (60–78%) compared with NaCl solution and control extractions (20–48% and 21%, respectively). The concentration of alkali or salt (0.25–1 mol L−1) had a minor but significant impact on the yield. Amino acid composition analysis revealed that hempseed meal contains 24% (54.5 ± 0.19 mg g−1) essential amino acids of total amino acids, and extraction with NaOH, KOH, NaHCO3 or NaCl did not improve the selective extraction of essential amino acids compared to control experiments. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis allowed the identification of edestin and albumin in the extracts obtained with NaHCO3 and NaCl solvents, with results further showing that the type of extraction solvent influences protein extraction selectivity. CONCLUSION: Although alkali solvents provide superior extraction yields, extraction with water resulted in extracts containing the highest proportion of proteins bearing essential amino acids. According to the results of SDS-PAGE, extraction using alkali solvents induced protein crosslinking.",
keywords = "alkali extraction, amino acid analysis, hemp proteins, plant-based proteins, salt extraction",
author = "Cabral, {Eduarda M.} and Poojary, {Mahesha M.} and Lund, {Marianne N.} and James Curtin and Mark Fenelon and Tiwari, {Brijesh K.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.",
year = "2022",
doi = "10.1002/jsfa.11979",
language = "English",
volume = "102",
pages = "6293--6298",
journal = "Journal of the Science of Food and Agriculture",
issn = "0022-5142",
publisher = "JohnWiley & Sons Ltd",
number = "14",

}

RIS

TY - JOUR

T1 - Effect of solvent composition on the extraction of proteins from hemp oil processing stream

AU - Cabral, Eduarda M.

AU - Poojary, Mahesha M.

AU - Lund, Marianne N.

AU - Curtin, James

AU - Fenelon, Mark

AU - Tiwari, Brijesh K.

N1 - Publisher Copyright: © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

PY - 2022

Y1 - 2022

N2 - BACKGROUND: Hempseed meal, a by-product of the hempseed oil processing stream, is a potential alternative source for food proteins. Efficient extraction of proteins from hempseed meal is challenging owing to differences in the structure and solubility of various protein fractions present in the seed. In the present study, protein was extracted from hempseed meal using four different solvents, including aqueous NaOH, KOH, NaHCO3 and NaCl, at four different concentrations with the aim of improving the recovery of protein fractions rich in essential amino acids. RESULTS: Extraction using alkaline solvents provided superior protein recovery (60–78%) compared with NaCl solution and control extractions (20–48% and 21%, respectively). The concentration of alkali or salt (0.25–1 mol L−1) had a minor but significant impact on the yield. Amino acid composition analysis revealed that hempseed meal contains 24% (54.5 ± 0.19 mg g−1) essential amino acids of total amino acids, and extraction with NaOH, KOH, NaHCO3 or NaCl did not improve the selective extraction of essential amino acids compared to control experiments. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis allowed the identification of edestin and albumin in the extracts obtained with NaHCO3 and NaCl solvents, with results further showing that the type of extraction solvent influences protein extraction selectivity. CONCLUSION: Although alkali solvents provide superior extraction yields, extraction with water resulted in extracts containing the highest proportion of proteins bearing essential amino acids. According to the results of SDS-PAGE, extraction using alkali solvents induced protein crosslinking.

AB - BACKGROUND: Hempseed meal, a by-product of the hempseed oil processing stream, is a potential alternative source for food proteins. Efficient extraction of proteins from hempseed meal is challenging owing to differences in the structure and solubility of various protein fractions present in the seed. In the present study, protein was extracted from hempseed meal using four different solvents, including aqueous NaOH, KOH, NaHCO3 and NaCl, at four different concentrations with the aim of improving the recovery of protein fractions rich in essential amino acids. RESULTS: Extraction using alkaline solvents provided superior protein recovery (60–78%) compared with NaCl solution and control extractions (20–48% and 21%, respectively). The concentration of alkali or salt (0.25–1 mol L−1) had a minor but significant impact on the yield. Amino acid composition analysis revealed that hempseed meal contains 24% (54.5 ± 0.19 mg g−1) essential amino acids of total amino acids, and extraction with NaOH, KOH, NaHCO3 or NaCl did not improve the selective extraction of essential amino acids compared to control experiments. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis allowed the identification of edestin and albumin in the extracts obtained with NaHCO3 and NaCl solvents, with results further showing that the type of extraction solvent influences protein extraction selectivity. CONCLUSION: Although alkali solvents provide superior extraction yields, extraction with water resulted in extracts containing the highest proportion of proteins bearing essential amino acids. According to the results of SDS-PAGE, extraction using alkali solvents induced protein crosslinking.

KW - alkali extraction

KW - amino acid analysis

KW - hemp proteins

KW - plant-based proteins

KW - salt extraction

U2 - 10.1002/jsfa.11979

DO - 10.1002/jsfa.11979

M3 - Journal article

C2 - 35514139

AN - SCOPUS:85130281626

VL - 102

SP - 6293

EP - 6298

JO - Journal of the Science of Food and Agriculture

JF - Journal of the Science of Food and Agriculture

SN - 0022-5142

IS - 14

ER -

ID: 310574308