Ceramide content is higher in type I compared to type II fibers in obesity and type 2 diabetes mellitus
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Ceramide content is higher in type I compared to type II fibers in obesity and type 2 diabetes mellitus. / Kristensen, Ditte Bech; Prats Gavalda, Clara; Larsen, Steen; Ara, Ignacio; Dela, Flemming; Helge, Jørn W; Larsen, Steen.
In: Acta Diabetologica, 2012.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Ceramide content is higher in type I compared to type II fibers in obesity and type 2 diabetes mellitus
AU - Kristensen, Ditte Bech
AU - Prats Gavalda, Clara
AU - Larsen, Steen
AU - Ara, Ignacio
AU - Dela, Flemming
AU - Helge, Jørn W
AU - Larsen, Steen
PY - 2012
Y1 - 2012
N2 - This study investigated fiber-type-specific muscle ceramide content in obese subjects and type 2 diabetes patients. Two substudies, one which compared type 2 diabetes patients to both lean- and obese BMI-matched subjects and the other study which compared lean body-matched post-obese, obese, and control subjects, were performed. A fasting blood sample was obtained and plasma insulin and glucose determined. A muscle biopsy was obtained from deltoideus and vastus lateralis, and fiber-type ceramide content was determined by fluorescence immunohistochemistry. Insulin sensitivity estimated by Quicki index was higher in lean compared to type 2 diabetes patients and obese controls. Also in control and post-obese subjects, a higher insulin sensitivity was observed compared to obese subjects. Ceramide content was consistently higher in type I than in type II muscle fibers and higher in deltoideus than vastus lateralis across all groups. No significant differences between groups were observed in ceramide content in either of the two substudies. In human skeletal muscle, ceramide content was higher in type I than in type II fibers in patients with type 2 diabetes and in obese subjects, but overall ceramide muscle fiber content was not different compared to controls.
AB - This study investigated fiber-type-specific muscle ceramide content in obese subjects and type 2 diabetes patients. Two substudies, one which compared type 2 diabetes patients to both lean- and obese BMI-matched subjects and the other study which compared lean body-matched post-obese, obese, and control subjects, were performed. A fasting blood sample was obtained and plasma insulin and glucose determined. A muscle biopsy was obtained from deltoideus and vastus lateralis, and fiber-type ceramide content was determined by fluorescence immunohistochemistry. Insulin sensitivity estimated by Quicki index was higher in lean compared to type 2 diabetes patients and obese controls. Also in control and post-obese subjects, a higher insulin sensitivity was observed compared to obese subjects. Ceramide content was consistently higher in type I than in type II muscle fibers and higher in deltoideus than vastus lateralis across all groups. No significant differences between groups were observed in ceramide content in either of the two substudies. In human skeletal muscle, ceramide content was higher in type I than in type II fibers in patients with type 2 diabetes and in obese subjects, but overall ceramide muscle fiber content was not different compared to controls.
U2 - 10.1007/s00592-012-0379-0
DO - 10.1007/s00592-012-0379-0
M3 - Journal article
C2 - 22350135
JO - Acta Diabetologica
JF - Acta Diabetologica
SN - 0940-5429
ER -
ID: 40253549