Cellular responses to radical propagation from ion-implanted plasma polymer surfaces
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Cellular responses to radical propagation from ion-implanted plasma polymer surfaces. / Stewart, Callum A. C.; Akhavan, Behnam; Santos, Miguel; Hung, JuiChien; Hawkins, Clare L.; Bao, Shisan; Wise, Steven G.; Bilek, Marcela M. M.
In: Applied Surface Science, Vol. 456, 2018, p. 701-710.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cellular responses to radical propagation from ion-implanted plasma polymer surfaces
AU - Stewart, Callum A. C.
AU - Akhavan, Behnam
AU - Santos, Miguel
AU - Hung, JuiChien
AU - Hawkins, Clare L.
AU - Bao, Shisan
AU - Wise, Steven G.
AU - Bilek, Marcela M. M.
PY - 2018
Y1 - 2018
N2 - Biomolecule-functionalization, through the presentation of biological motifs that promote optimal cellular responses, has the capacity to improve the tissue integration of biomedical devices and hence patients' quality of life. Radical-functionalized plasma polymer films (rPPFs) readily immobilize bioactive molecules on exposure to a biomolecule-containing aqueous solution without the need for chemical reagents. However, the potential for damage to cells and tissues due to the high local concentration of radicals in freshly deposited radical-functionalized plasma polymer films is of concern. In this study, we compared a fresh (4 h post-deposition) rPPF with one that had been aged for 11 days to explore the effect of the different radical fluxes on cellular responses. Primary osteoblasts and MG63 bone osteosarcoma cells were used to determine whether rPPFs at early aging times exhibited radical-induced cytotoxicity. The aging behavior of the rPPFs demonstrated a connection between the radical decay kinetics and the surface chemistry and wettability. Significant increases in cell attachment and spreading compared to bare Ti were observed for both cell lineages on the rPPF surfaces. The proliferation assays showed equivalent proliferation rates on both the fresh and aged surfaces, and no evidence of cytotoxicity was observed. Overall, we demonstrated that the high flux of radicals emerging to the surface has minimal influence on the biocompatibility of radical-functionalized plasma polymer films.
AB - Biomolecule-functionalization, through the presentation of biological motifs that promote optimal cellular responses, has the capacity to improve the tissue integration of biomedical devices and hence patients' quality of life. Radical-functionalized plasma polymer films (rPPFs) readily immobilize bioactive molecules on exposure to a biomolecule-containing aqueous solution without the need for chemical reagents. However, the potential for damage to cells and tissues due to the high local concentration of radicals in freshly deposited radical-functionalized plasma polymer films is of concern. In this study, we compared a fresh (4 h post-deposition) rPPF with one that had been aged for 11 days to explore the effect of the different radical fluxes on cellular responses. Primary osteoblasts and MG63 bone osteosarcoma cells were used to determine whether rPPFs at early aging times exhibited radical-induced cytotoxicity. The aging behavior of the rPPFs demonstrated a connection between the radical decay kinetics and the surface chemistry and wettability. Significant increases in cell attachment and spreading compared to bare Ti were observed for both cell lineages on the rPPF surfaces. The proliferation assays showed equivalent proliferation rates on both the fresh and aged surfaces, and no evidence of cytotoxicity was observed. Overall, we demonstrated that the high flux of radicals emerging to the surface has minimal influence on the biocompatibility of radical-functionalized plasma polymer films.
KW - Radical flux
KW - Plasma polymer films
KW - Biocompatibility
KW - Ion-bombardment
KW - Osteoblasts
U2 - 10.1016/j.apsusc.2018.06.111
DO - 10.1016/j.apsusc.2018.06.111
M3 - Journal article
VL - 456
SP - 701
EP - 710
JO - Applied Surface Science
JF - Applied Surface Science
SN - 0169-4332
ER -
ID: 212862596