Cell swelling activates cloned Ca(2+)-activated K(+) channels: a role for the F-actin cytoskeleton.

Research output: Contribution to journalJournal articleResearchpeer-review

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Cell swelling activates cloned Ca(2+)-activated K(+) channels: a role for the F-actin cytoskeleton. / Jorgensen, Nanna K; Pedersen, Stine F; Rasmussen, Hanne B; Grunnet, Morten; Klaerke, Dan A; Olesen, Søren-Peter.

In: BBA General Subjects, Vol. 1615, No. 1-2, 2003, p. 115-25.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Jorgensen, NK, Pedersen, SF, Rasmussen, HB, Grunnet, M, Klaerke, DA & Olesen, S-P 2003, 'Cell swelling activates cloned Ca(2+)-activated K(+) channels: a role for the F-actin cytoskeleton.', BBA General Subjects, vol. 1615, no. 1-2, pp. 115-25.

APA

Jorgensen, N. K., Pedersen, S. F., Rasmussen, H. B., Grunnet, M., Klaerke, D. A., & Olesen, S-P. (2003). Cell swelling activates cloned Ca(2+)-activated K(+) channels: a role for the F-actin cytoskeleton. BBA General Subjects, 1615(1-2), 115-25.

Vancouver

Jorgensen NK, Pedersen SF, Rasmussen HB, Grunnet M, Klaerke DA, Olesen S-P. Cell swelling activates cloned Ca(2+)-activated K(+) channels: a role for the F-actin cytoskeleton. BBA General Subjects. 2003;1615(1-2):115-25.

Author

Jorgensen, Nanna K ; Pedersen, Stine F ; Rasmussen, Hanne B ; Grunnet, Morten ; Klaerke, Dan A ; Olesen, Søren-Peter. / Cell swelling activates cloned Ca(2+)-activated K(+) channels: a role for the F-actin cytoskeleton. In: BBA General Subjects. 2003 ; Vol. 1615, No. 1-2. pp. 115-25.

Bibtex

@article{600abff0ab5511ddb5e9000ea68e967b,
title = "Cell swelling activates cloned Ca(2+)-activated K(+) channels: a role for the F-actin cytoskeleton.",
abstract = "Cloned Ca(2+)-activated K(+) channels of intermediate (hIK) or small (rSK3) conductance were expressed in HEK 293 cells, and channel activity was monitored using whole-cell patch clamp. hIK and rSK3 currents already activated by intracellular calcium were further increased by 95{\%} and 125{\%}, respectively, upon exposure of the cells to a 33{\%} decrease in extracellular osmolarity. hIK and rSK3 currents were inhibited by 46{\%} and 32{\%}, respectively, by a 50{\%} increase in extracellular osmolarity. Cell swelling and channel activation were not associated with detectable increases in [Ca(2+)](i), evidenced by population and single-cell measurements. In addition, inhibitors of IK and SK channels significantly reduced the rate of regulatory volume decrease (RVD) in cells expressing these channels. Cell swelling induced a decrease, and cell shrinkage an increase, in net cellular F-actin content. The swelling-induced activation of hIK channels was strongly inhibited by cytochalasin D (CD), in concentrations that caused depolymerization of F-actin filaments, indicating a role for the F-actin cytoskeleton in modulation of hIK by changes in cell volume. In conclusion, hIK and rSK3 channels are activated by cell swelling and inhibited by shrinkage. A role for the F-actin cytoskeleton in the swelling-induced activation of hIK channels is suggested.",
author = "Jorgensen, {Nanna K} and Pedersen, {Stine F} and Rasmussen, {Hanne B} and Morten Grunnet and Klaerke, {Dan A} and S{\o}ren-Peter Olesen",
note = "Keywords: Actins; Calcium; Cell Size; Cloning, Molecular; Cytochalasin D; Cytoskeleton; Humans; Kidney; Nucleic Acid Synthesis Inhibitors; Potassium Channels, Calcium-Activated",
year = "2003",
language = "English",
volume = "1615",
pages = "115--25",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Cell swelling activates cloned Ca(2+)-activated K(+) channels: a role for the F-actin cytoskeleton.

AU - Jorgensen, Nanna K

AU - Pedersen, Stine F

AU - Rasmussen, Hanne B

AU - Grunnet, Morten

AU - Klaerke, Dan A

AU - Olesen, Søren-Peter

N1 - Keywords: Actins; Calcium; Cell Size; Cloning, Molecular; Cytochalasin D; Cytoskeleton; Humans; Kidney; Nucleic Acid Synthesis Inhibitors; Potassium Channels, Calcium-Activated

PY - 2003

Y1 - 2003

N2 - Cloned Ca(2+)-activated K(+) channels of intermediate (hIK) or small (rSK3) conductance were expressed in HEK 293 cells, and channel activity was monitored using whole-cell patch clamp. hIK and rSK3 currents already activated by intracellular calcium were further increased by 95% and 125%, respectively, upon exposure of the cells to a 33% decrease in extracellular osmolarity. hIK and rSK3 currents were inhibited by 46% and 32%, respectively, by a 50% increase in extracellular osmolarity. Cell swelling and channel activation were not associated with detectable increases in [Ca(2+)](i), evidenced by population and single-cell measurements. In addition, inhibitors of IK and SK channels significantly reduced the rate of regulatory volume decrease (RVD) in cells expressing these channels. Cell swelling induced a decrease, and cell shrinkage an increase, in net cellular F-actin content. The swelling-induced activation of hIK channels was strongly inhibited by cytochalasin D (CD), in concentrations that caused depolymerization of F-actin filaments, indicating a role for the F-actin cytoskeleton in modulation of hIK by changes in cell volume. In conclusion, hIK and rSK3 channels are activated by cell swelling and inhibited by shrinkage. A role for the F-actin cytoskeleton in the swelling-induced activation of hIK channels is suggested.

AB - Cloned Ca(2+)-activated K(+) channels of intermediate (hIK) or small (rSK3) conductance were expressed in HEK 293 cells, and channel activity was monitored using whole-cell patch clamp. hIK and rSK3 currents already activated by intracellular calcium were further increased by 95% and 125%, respectively, upon exposure of the cells to a 33% decrease in extracellular osmolarity. hIK and rSK3 currents were inhibited by 46% and 32%, respectively, by a 50% increase in extracellular osmolarity. Cell swelling and channel activation were not associated with detectable increases in [Ca(2+)](i), evidenced by population and single-cell measurements. In addition, inhibitors of IK and SK channels significantly reduced the rate of regulatory volume decrease (RVD) in cells expressing these channels. Cell swelling induced a decrease, and cell shrinkage an increase, in net cellular F-actin content. The swelling-induced activation of hIK channels was strongly inhibited by cytochalasin D (CD), in concentrations that caused depolymerization of F-actin filaments, indicating a role for the F-actin cytoskeleton in modulation of hIK by changes in cell volume. In conclusion, hIK and rSK3 channels are activated by cell swelling and inhibited by shrinkage. A role for the F-actin cytoskeleton in the swelling-induced activation of hIK channels is suggested.

M3 - Journal article

C2 - 12948593

VL - 1615

SP - 115

EP - 125

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 1-2

ER -

ID: 8418923