Cell culture plastics with immobilized interleukin-4 for monocyte differentiation

Research output: Contribution to journalJournal articleResearchpeer-review

Standard cell culture plastic was surface modified by passive adsorption or covalent attachment of interleukin (IL)-4 and investigated for its ability to induce differentiation of human monocytes into mature dendritic cells, a process dose-dependently regulated by IL-4. Covalent attachment of IL-4 proceeded via anthraquinone photochemistry to introduce amine functionalities at the surface followed by coupling of IL-4 through a bifunctional amine-reactive linker. X-ray photoelectron spectroscopy showed that undesirable multilayer formation of the photoactive compound could be avoided by reaction in water instead of phosphate-buffered saline. Passively adsorbed IL-4 was observed to induce differentiation to dendritic cells, but analysis of cell culture supernatants revealed that leakage of IL-4 into solution could account for the differentiation observed. Covalent attachment resulted in bound IL-4 at similar concentrations to the passive adsorption process, as measured by enzyme-linked immunosorbent assays, and the bound IL-4 did not leak into solution to any measurable extent during cell culture. However, covalently bound IL-4 was incapable of inducing monocyte differentiation. This may be caused by IL-4 denaturation or improper epitope presentation induced by the immobilization process, or by biological irresponsiveness of monocytes to IL-4 in immobilized formats.

Original languageEnglish
JournalJournal of Biomedical Materials Research. Part A
Issue number2
Pages (from-to)372-83
Number of pages12
Publication statusPublished - Feb 2011

    Research areas

  • Adsorption, Anthraquinones, Cell Culture Techniques, Cell Differentiation, Dendritic Cells, Down-Regulation, Enzyme-Linked Immunosorbent Assay, Humans, Immobilized Proteins, Interleukin-4, Monocytes, Plastics, Solutions, Surface Properties, Triazines, Up-Regulation, Journal Article, Research Support, Non-U.S. Gov't

ID: 184774279