Cardiac spheroids as promising in vitro models to study the human heart microenvironment

Research output: Contribution to journalJournal articleResearchpeer-review

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Cardiac spheroids as promising in vitro models to study the human heart microenvironment. / Polonchuk, Liudmila; Chabria, Mamta; Badi, Laura; Hoflack, Jean-Christophe; Figtree, Gemma A; Davies, Michael J; Gentile, Carmine.

In: Scientific Reports, Vol. 7, 7005, 01.08.2017.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Polonchuk, L, Chabria, M, Badi, L, Hoflack, J-C, Figtree, GA, Davies, MJ & Gentile, C 2017, 'Cardiac spheroids as promising in vitro models to study the human heart microenvironment', Scientific Reports, vol. 7, 7005. https://doi.org/10.1038/s41598-017-06385-8

APA

Polonchuk, L., Chabria, M., Badi, L., Hoflack, J-C., Figtree, G. A., Davies, M. J., & Gentile, C. (2017). Cardiac spheroids as promising in vitro models to study the human heart microenvironment. Scientific Reports, 7, [7005]. https://doi.org/10.1038/s41598-017-06385-8

Vancouver

Polonchuk L, Chabria M, Badi L, Hoflack J-C, Figtree GA, Davies MJ et al. Cardiac spheroids as promising in vitro models to study the human heart microenvironment. Scientific Reports. 2017 Aug 1;7. 7005. https://doi.org/10.1038/s41598-017-06385-8

Author

Polonchuk, Liudmila ; Chabria, Mamta ; Badi, Laura ; Hoflack, Jean-Christophe ; Figtree, Gemma A ; Davies, Michael J ; Gentile, Carmine. / Cardiac spheroids as promising in vitro models to study the human heart microenvironment. In: Scientific Reports. 2017 ; Vol. 7.

Bibtex

@article{60dcd39c55224d66a130ccd51550a91c,
title = "Cardiac spheroids as promising in vitro models to study the human heart microenvironment",
abstract = "Three-dimensional in vitro cell systems are a promising alternative to animals to study cardiac biology and disease. We have generated three-dimensional in vitro models of the human heart ({"}cardiac spheroids{"}, CSs) by co-culturing human primary or iPSC-derived cardiomyocytes, endothelial cells and fibroblasts at ratios approximating those present in vivo. The cellular organisation, extracellular matrix and microvascular network mimic human heart tissue. These spheroids have been employed to investigate the dose-limiting cardiotoxicity of the common anti-cancer drug doxorubicin. Viability/cytotoxicity assays indicate dose-dependent cytotoxic effects, which are inhibited by the nitric oxide synthase (NOS) inhibitor L-NIO, and genetic inhibition of endothelial NOS, implicating peroxynitrous acid as a key damaging agent. These data indicate that CSs mimic important features of human heart morphology, biochemistry and pharmacology in vitro, offering a promising alternative to animals and standard cell cultures with regard to mechanistic insights and prediction of toxic effects in human heart tissue.",
author = "Liudmila Polonchuk and Mamta Chabria and Laura Badi and Jean-Christophe Hoflack and Figtree, {Gemma A} and Davies, {Michael J} and Carmine Gentile",
year = "2017",
month = "8",
day = "1",
doi = "10.1038/s41598-017-06385-8",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Cardiac spheroids as promising in vitro models to study the human heart microenvironment

AU - Polonchuk, Liudmila

AU - Chabria, Mamta

AU - Badi, Laura

AU - Hoflack, Jean-Christophe

AU - Figtree, Gemma A

AU - Davies, Michael J

AU - Gentile, Carmine

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Three-dimensional in vitro cell systems are a promising alternative to animals to study cardiac biology and disease. We have generated three-dimensional in vitro models of the human heart ("cardiac spheroids", CSs) by co-culturing human primary or iPSC-derived cardiomyocytes, endothelial cells and fibroblasts at ratios approximating those present in vivo. The cellular organisation, extracellular matrix and microvascular network mimic human heart tissue. These spheroids have been employed to investigate the dose-limiting cardiotoxicity of the common anti-cancer drug doxorubicin. Viability/cytotoxicity assays indicate dose-dependent cytotoxic effects, which are inhibited by the nitric oxide synthase (NOS) inhibitor L-NIO, and genetic inhibition of endothelial NOS, implicating peroxynitrous acid as a key damaging agent. These data indicate that CSs mimic important features of human heart morphology, biochemistry and pharmacology in vitro, offering a promising alternative to animals and standard cell cultures with regard to mechanistic insights and prediction of toxic effects in human heart tissue.

AB - Three-dimensional in vitro cell systems are a promising alternative to animals to study cardiac biology and disease. We have generated three-dimensional in vitro models of the human heart ("cardiac spheroids", CSs) by co-culturing human primary or iPSC-derived cardiomyocytes, endothelial cells and fibroblasts at ratios approximating those present in vivo. The cellular organisation, extracellular matrix and microvascular network mimic human heart tissue. These spheroids have been employed to investigate the dose-limiting cardiotoxicity of the common anti-cancer drug doxorubicin. Viability/cytotoxicity assays indicate dose-dependent cytotoxic effects, which are inhibited by the nitric oxide synthase (NOS) inhibitor L-NIO, and genetic inhibition of endothelial NOS, implicating peroxynitrous acid as a key damaging agent. These data indicate that CSs mimic important features of human heart morphology, biochemistry and pharmacology in vitro, offering a promising alternative to animals and standard cell cultures with regard to mechanistic insights and prediction of toxic effects in human heart tissue.

U2 - 10.1038/s41598-017-06385-8

DO - 10.1038/s41598-017-06385-8

M3 - Journal article

C2 - 28765558

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 7005

ER -

ID: 182331016