Acute erythropoietin injection increases muscle mitochondrial respiratory capacity in young men: A double-blinded randomized crossover trial

Research output: Contribution to journalJournal articlepeer-review

Standard

Acute erythropoietin injection increases muscle mitochondrial respiratory capacity in young men : A double-blinded randomized crossover trial. / Larsen, Steen; Søndergård, Stine Dam; Sahl, Ronni Eg; Frandsen, Jacob; Morville, Thomas; Dela, Flemming; Helge, Jørn W.

In: Journal of Applied Physiology, Vol. 131, No. 4, 2021, p. 1340-1347.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Larsen, S, Søndergård, SD, Sahl, RE, Frandsen, J, Morville, T, Dela, F & Helge, JW 2021, 'Acute erythropoietin injection increases muscle mitochondrial respiratory capacity in young men: A double-blinded randomized crossover trial', Journal of Applied Physiology, vol. 131, no. 4, pp. 1340-1347. https://doi.org/10.1152/japplphysiol.00995.2020

APA

Larsen, S., Søndergård, S. D., Sahl, R. E., Frandsen, J., Morville, T., Dela, F., & Helge, J. W. (2021). Acute erythropoietin injection increases muscle mitochondrial respiratory capacity in young men: A double-blinded randomized crossover trial. Journal of Applied Physiology, 131(4), 1340-1347. https://doi.org/10.1152/japplphysiol.00995.2020

Vancouver

Larsen S, Søndergård SD, Sahl RE, Frandsen J, Morville T, Dela F et al. Acute erythropoietin injection increases muscle mitochondrial respiratory capacity in young men: A double-blinded randomized crossover trial. Journal of Applied Physiology. 2021;131(4):1340-1347. https://doi.org/10.1152/japplphysiol.00995.2020

Author

Larsen, Steen ; Søndergård, Stine Dam ; Sahl, Ronni Eg ; Frandsen, Jacob ; Morville, Thomas ; Dela, Flemming ; Helge, Jørn W. / Acute erythropoietin injection increases muscle mitochondrial respiratory capacity in young men : A double-blinded randomized crossover trial. In: Journal of Applied Physiology. 2021 ; Vol. 131, No. 4. pp. 1340-1347.

Bibtex

@article{5b4547485aa74bbebd2592cdd8d39ef0,
title = "Acute erythropoietin injection increases muscle mitochondrial respiratory capacity in young men: A double-blinded randomized crossover trial",
abstract = "The aim was to investigate if acute recombinant human erythropoietin (rHuEPO) injection had an effect on mitochondrial function and if exercise would have an additive effect. Furthermore, to investigate if in vitro incubation with rHuEPO had an effect on muscle mitochondrial respiratory capacity. Eight healthy young men were recruited for this double-blinded randomized placebocontrolled crossover study. rHuEPO (400 IU/kg body wt) or saline injection was given intravenously, before an acute bout of exercise. Resting metabolic rate and fat oxidation were measured. Biopsies were obtained at baseline, 120 min after injection, and right after the acute exercise bout. Mitochondrial function (mitochondrial respiration and H2O2 emission) was measured in permeabilized skeletal muscle using high-resolution respirometry and fluorometry. Specific gene expression and enzyme activity were measured. Skeletal muscle mitochondrial respiratory capacity was measured with and without incubation with rHuEPO. Fat oxidation at rest increased after rHuEPO injection, but no difference was found in fat oxidation during exercise. Mitochondrial respiratory capacity was increased after rHuEPO injection when pyruvate was in the assay, which was not the case when saline was injected. No changes were seen in H2O2 emission after rHuEPO injection or acute exercise. Incubation of skeletal muscle fibers in vitro with rHuEPO increased mitochondrial respiratory capacity. Acute rHuEPO injection increased mitochondrial respiratory capacity when pyruvate was used in the assay. No statistical difference was found in H2O2 emission capacity, although a numerical increase was seen after rHuEPO injection. In vitro incubation of the skeletal muscle sample with rHuEPO increases mitochondrial respiratory capacity. ",
keywords = "EPO, Exercise, HOemission, Human, Mitochondrial biogenesis",
author = "Steen Larsen and S{\o}nderg{\aa}rd, {Stine Dam} and Sahl, {Ronni Eg} and Jacob Frandsen and Thomas Morville and Flemming Dela and Helge, {J{\o}rn W.}",
note = "Publisher Copyright: Copyright {\textcopyright} 2021 the American Physiological Society.",
year = "2021",
doi = "10.1152/japplphysiol.00995.2020",
language = "English",
volume = "131",
pages = "1340--1347",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "4",

}

RIS

TY - JOUR

T1 - Acute erythropoietin injection increases muscle mitochondrial respiratory capacity in young men

T2 - A double-blinded randomized crossover trial

AU - Larsen, Steen

AU - Søndergård, Stine Dam

AU - Sahl, Ronni Eg

AU - Frandsen, Jacob

AU - Morville, Thomas

AU - Dela, Flemming

AU - Helge, Jørn W.

N1 - Publisher Copyright: Copyright © 2021 the American Physiological Society.

PY - 2021

Y1 - 2021

N2 - The aim was to investigate if acute recombinant human erythropoietin (rHuEPO) injection had an effect on mitochondrial function and if exercise would have an additive effect. Furthermore, to investigate if in vitro incubation with rHuEPO had an effect on muscle mitochondrial respiratory capacity. Eight healthy young men were recruited for this double-blinded randomized placebocontrolled crossover study. rHuEPO (400 IU/kg body wt) or saline injection was given intravenously, before an acute bout of exercise. Resting metabolic rate and fat oxidation were measured. Biopsies were obtained at baseline, 120 min after injection, and right after the acute exercise bout. Mitochondrial function (mitochondrial respiration and H2O2 emission) was measured in permeabilized skeletal muscle using high-resolution respirometry and fluorometry. Specific gene expression and enzyme activity were measured. Skeletal muscle mitochondrial respiratory capacity was measured with and without incubation with rHuEPO. Fat oxidation at rest increased after rHuEPO injection, but no difference was found in fat oxidation during exercise. Mitochondrial respiratory capacity was increased after rHuEPO injection when pyruvate was in the assay, which was not the case when saline was injected. No changes were seen in H2O2 emission after rHuEPO injection or acute exercise. Incubation of skeletal muscle fibers in vitro with rHuEPO increased mitochondrial respiratory capacity. Acute rHuEPO injection increased mitochondrial respiratory capacity when pyruvate was used in the assay. No statistical difference was found in H2O2 emission capacity, although a numerical increase was seen after rHuEPO injection. In vitro incubation of the skeletal muscle sample with rHuEPO increases mitochondrial respiratory capacity.

AB - The aim was to investigate if acute recombinant human erythropoietin (rHuEPO) injection had an effect on mitochondrial function and if exercise would have an additive effect. Furthermore, to investigate if in vitro incubation with rHuEPO had an effect on muscle mitochondrial respiratory capacity. Eight healthy young men were recruited for this double-blinded randomized placebocontrolled crossover study. rHuEPO (400 IU/kg body wt) or saline injection was given intravenously, before an acute bout of exercise. Resting metabolic rate and fat oxidation were measured. Biopsies were obtained at baseline, 120 min after injection, and right after the acute exercise bout. Mitochondrial function (mitochondrial respiration and H2O2 emission) was measured in permeabilized skeletal muscle using high-resolution respirometry and fluorometry. Specific gene expression and enzyme activity were measured. Skeletal muscle mitochondrial respiratory capacity was measured with and without incubation with rHuEPO. Fat oxidation at rest increased after rHuEPO injection, but no difference was found in fat oxidation during exercise. Mitochondrial respiratory capacity was increased after rHuEPO injection when pyruvate was in the assay, which was not the case when saline was injected. No changes were seen in H2O2 emission after rHuEPO injection or acute exercise. Incubation of skeletal muscle fibers in vitro with rHuEPO increased mitochondrial respiratory capacity. Acute rHuEPO injection increased mitochondrial respiratory capacity when pyruvate was used in the assay. No statistical difference was found in H2O2 emission capacity, although a numerical increase was seen after rHuEPO injection. In vitro incubation of the skeletal muscle sample with rHuEPO increases mitochondrial respiratory capacity.

KW - EPO

KW - Exercise

KW - HOemission

KW - Human

KW - Mitochondrial biogenesis

U2 - 10.1152/japplphysiol.00995.2020

DO - 10.1152/japplphysiol.00995.2020

M3 - Journal article

C2 - 34498946

AN - SCOPUS:85117179466

VL - 131

SP - 1340

EP - 1347

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 4

ER -

ID: 283749618