A Human Variant of Glucose-Regulated Protein 94 That Inefficiently Supports IGF Production

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A Human Variant of Glucose-Regulated Protein 94 That Inefficiently Supports IGF Production. / Marzec, Michal; Hawkes, Colin P; Eletto, Davide; Boyle, Sarah; Rosenfeld, Ron G; Hwa, Vivian; Wit, Jan M; van Duyvenvoorde, Hermine A; Oostdijk, Wilma; Losekoot, Monique; Pedersen, Oluf; Yeap, Bu Beng; Flicker, Leon; Barzilai, Nir; Atzmon, Gil; Grimberg, Adda; Argon, Yair.

In: Endocrinology, Vol. 157, No. 5, 05.2016, p. 1914-1928.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Marzec, M, Hawkes, CP, Eletto, D, Boyle, S, Rosenfeld, RG, Hwa, V, Wit, JM, van Duyvenvoorde, HA, Oostdijk, W, Losekoot, M, Pedersen, O, Yeap, BB, Flicker, L, Barzilai, N, Atzmon, G, Grimberg, A & Argon, Y 2016, 'A Human Variant of Glucose-Regulated Protein 94 That Inefficiently Supports IGF Production', Endocrinology, vol. 157, no. 5, pp. 1914-1928. https://doi.org/10.1210/en.2015-2058

APA

Marzec, M., Hawkes, C. P., Eletto, D., Boyle, S., Rosenfeld, R. G., Hwa, V., ... Argon, Y. (2016). A Human Variant of Glucose-Regulated Protein 94 That Inefficiently Supports IGF Production. Endocrinology, 157(5), 1914-1928. https://doi.org/10.1210/en.2015-2058

Vancouver

Marzec M, Hawkes CP, Eletto D, Boyle S, Rosenfeld RG, Hwa V et al. A Human Variant of Glucose-Regulated Protein 94 That Inefficiently Supports IGF Production. Endocrinology. 2016 May;157(5):1914-1928. https://doi.org/10.1210/en.2015-2058

Author

Marzec, Michal ; Hawkes, Colin P ; Eletto, Davide ; Boyle, Sarah ; Rosenfeld, Ron G ; Hwa, Vivian ; Wit, Jan M ; van Duyvenvoorde, Hermine A ; Oostdijk, Wilma ; Losekoot, Monique ; Pedersen, Oluf ; Yeap, Bu Beng ; Flicker, Leon ; Barzilai, Nir ; Atzmon, Gil ; Grimberg, Adda ; Argon, Yair. / A Human Variant of Glucose-Regulated Protein 94 That Inefficiently Supports IGF Production. In: Endocrinology. 2016 ; Vol. 157, No. 5. pp. 1914-1928.

Bibtex

@article{23cbb069b79147cd81f7f2648c21e154,
title = "A Human Variant of Glucose-Regulated Protein 94 That Inefficiently Supports IGF Production",
abstract = "IGFs are critical for normal intrauterine and childhood growth and sustaining health throughout life. We showed previously that the production of IGF-1 and IGF-2 requires interaction with the chaperone glucose-regulated protein 94 (GRP94) and that the amount of secreted IGFs is proportional to the GRP94 activity. Therefore, we tested the hypothesis that functional polymorphisms of human GRP94 affect IGF production and thereby human health. We describe a hypomorphic variant of human GRP94, P300L, whose heterozygous carriers have 9{\%} lower circulating IGF-1 concentration. P300L was found first in a child with primary IGF deficiency and was later shown to be a noncommon single-nucleotide polymorphism with frequencies of 1{\%}-4{\%} in various populations. When tested in the grp94(-/-) cell-based complementation assay, P300L supported only approximately 58{\%} of IGF secretion relative to wild-type GRP94. Furthermore, recombinant P300L showed impaired nucleotide binding activity. These in vitro data strongly support a causal relationship between the GRP94 variant and the decreased concentration of circulating IGF-1, as observed in human carriers of P300L. Thus, mutations in GRP94 that affect its IGF chaperone activity represent a novel causal genetic mechanism that limits IGF biosynthesis, quite a distinct mechanism from the known genes in the GH/IGF signaling network.",
author = "Michal Marzec and Hawkes, {Colin P} and Davide Eletto and Sarah Boyle and Rosenfeld, {Ron G} and Vivian Hwa and Wit, {Jan M} and {van Duyvenvoorde}, {Hermine A} and Wilma Oostdijk and Monique Losekoot and Oluf Pedersen and Yeap, {Bu Beng} and Leon Flicker and Nir Barzilai and Gil Atzmon and Adda Grimberg and Yair Argon",
year = "2016",
month = "5",
doi = "10.1210/en.2015-2058",
language = "English",
volume = "157",
pages = "1914--1928",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - A Human Variant of Glucose-Regulated Protein 94 That Inefficiently Supports IGF Production

AU - Marzec, Michal

AU - Hawkes, Colin P

AU - Eletto, Davide

AU - Boyle, Sarah

AU - Rosenfeld, Ron G

AU - Hwa, Vivian

AU - Wit, Jan M

AU - van Duyvenvoorde, Hermine A

AU - Oostdijk, Wilma

AU - Losekoot, Monique

AU - Pedersen, Oluf

AU - Yeap, Bu Beng

AU - Flicker, Leon

AU - Barzilai, Nir

AU - Atzmon, Gil

AU - Grimberg, Adda

AU - Argon, Yair

PY - 2016/5

Y1 - 2016/5

N2 - IGFs are critical for normal intrauterine and childhood growth and sustaining health throughout life. We showed previously that the production of IGF-1 and IGF-2 requires interaction with the chaperone glucose-regulated protein 94 (GRP94) and that the amount of secreted IGFs is proportional to the GRP94 activity. Therefore, we tested the hypothesis that functional polymorphisms of human GRP94 affect IGF production and thereby human health. We describe a hypomorphic variant of human GRP94, P300L, whose heterozygous carriers have 9% lower circulating IGF-1 concentration. P300L was found first in a child with primary IGF deficiency and was later shown to be a noncommon single-nucleotide polymorphism with frequencies of 1%-4% in various populations. When tested in the grp94(-/-) cell-based complementation assay, P300L supported only approximately 58% of IGF secretion relative to wild-type GRP94. Furthermore, recombinant P300L showed impaired nucleotide binding activity. These in vitro data strongly support a causal relationship between the GRP94 variant and the decreased concentration of circulating IGF-1, as observed in human carriers of P300L. Thus, mutations in GRP94 that affect its IGF chaperone activity represent a novel causal genetic mechanism that limits IGF biosynthesis, quite a distinct mechanism from the known genes in the GH/IGF signaling network.

AB - IGFs are critical for normal intrauterine and childhood growth and sustaining health throughout life. We showed previously that the production of IGF-1 and IGF-2 requires interaction with the chaperone glucose-regulated protein 94 (GRP94) and that the amount of secreted IGFs is proportional to the GRP94 activity. Therefore, we tested the hypothesis that functional polymorphisms of human GRP94 affect IGF production and thereby human health. We describe a hypomorphic variant of human GRP94, P300L, whose heterozygous carriers have 9% lower circulating IGF-1 concentration. P300L was found first in a child with primary IGF deficiency and was later shown to be a noncommon single-nucleotide polymorphism with frequencies of 1%-4% in various populations. When tested in the grp94(-/-) cell-based complementation assay, P300L supported only approximately 58% of IGF secretion relative to wild-type GRP94. Furthermore, recombinant P300L showed impaired nucleotide binding activity. These in vitro data strongly support a causal relationship between the GRP94 variant and the decreased concentration of circulating IGF-1, as observed in human carriers of P300L. Thus, mutations in GRP94 that affect its IGF chaperone activity represent a novel causal genetic mechanism that limits IGF biosynthesis, quite a distinct mechanism from the known genes in the GH/IGF signaling network.

U2 - 10.1210/en.2015-2058

DO - 10.1210/en.2015-2058

M3 - Journal article

C2 - 26982636

VL - 157

SP - 1914

EP - 1928

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 5

ER -

ID: 178744864