Lola Julie Torz Pedersen

Education

Ph.D. on neuronal control of metabolism, at Panum Institute, Department of Biomedicine and Novo Nordisk Foundation Center for Basic Metabolic Research, section for Liver Metabolism and Metabolic Imaging, Copenhagen University.

Advisor: Dr. Matthew Paul Gillum, Ph.D., associate Professor at Copenhagen University

Project: Investigating the anatomy and physiology of neurons that suppress sucrose consumption

February 2015 - present

Double degree in association with Wageningen University, Wageningen, the Netherlands and Groupe ISA, Lille, France

February 2013 – January 2015

Master of Biology, specialization Health and Disease

February 2013 – January 2015 

Wageningen University, Wageningen, the Netherlands

Thesis: the nicotinic acetylcholine receptors in undifferentiated pheochromocytoma PC12 cells

Supervisors: Prof. Jens. D. Mikkelsen, NRU, Copenhagen and Dr. Katja Teerds, Wageningen University, Wageningen, the Netherlands

Engineer degree of Food Science, Agriculture and Environment, Engineer student

September 2011 – January 2015

Groupe ISA (Grand Ecole), Agricultural Engineering School, Lille, France

Bachelor of Food Science, Agriculture and Environment

September 2009 – August 2011

Groupe ISA (Grand Ecole), Agricultural Engineering School, Lille, France

Working experience

Advanced Techniques in Molecular Neuroscience: July 2015

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA

Gained theoretical and practical experience on multiple techniques such as:

-        CRISPR technology (by Dr. Le Cong and Dr. Joe LoTurco)

-        Single cell transcriptomics (by Dr. Craig Nelson)

-        Brain bows and multispectral imaging (by Dr. Dawen Cai)

-        Lentivirus production and injection (by Dr. Bryan Luikart)

-        Epigenetics and miRNA (by Dr. Anne Schaefer)

-        In utero electroporation (by Dr. Brady Maher)

-        Single cell electroporation and imaging (by Dr. Kurt Haas)

-        Optogenetics and CLARITY (by Dr. Viviana Gradinaru)

-        TRAP (by Dr. Joe Dougherty)

Master thesis: February 2014 – January 2015

Neurobiology Research Unit (NRU), Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark

Focused on the nicotinic acetylcholine receptors in undifferentiated pheochromocytoma PC12 cells using western blot, fluorescent immunocytochemistry and cell culture

Research assistant: June 2013

Experimental Zoology group, Department of Animal Sciences, Wageningen University, Wageningen, the Netherlands

Three-dimensional architecture of the Zebrafish trunk muscle at different stage of the embryonic development

Preparation of embryonic Zebrafish and microscopic analysis with Zeiss LSM-510 confocal microscope

Laboratory research assistant: July 2012 – August 2012

Muséum National d’Histoire Naturelle of Paris, France

Molecular systemic and bar-coding of elasmobranch and teleostei fishes from the ‘POKER’ campaign

PCR preparation, data analysis, data base (BOLDsystem, GenBank, FishBase) management and analysis

Skills related to my education

Cloning and design of GMO animal via plasmid injection (CRISPR technology)

Tissue clearing (CLARITY – PACT )

Extraction and measurement of protein, RNA and DNA level

Western blotting (gel electrophoresis, transfer blot and membranes analysis)

Immunofluorescence staining, microscopic slice preparation (cryostat, microtome, confocal and LCM)

Microglia and neuron isolation from rat pup brain

HEK293 cells, PC12 cells, microglia and neuron culture

FELASA categories ABD (previously called category C)

References

Dr. Matthew Paul Gillum,

Ph.D., associate Professor at Copenhagen University

gillum@sund.ku.dk

+45 23 64 89 95

Dr. Kitt Mia Falk Petersen, M.D. 

Professor of Internal Medicine at Yale University

Kitt.petersen@yale.edu

203-737-1942

Dr. Linda V. Blomster,

PhD, post-doc at NRU

linda.blomster@nru.dk

+46 763 110 091