Variation in mitochondrial respiratory capacity and myosin heavy chain composition in repeated muscle biopsies

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Standard

Variation in mitochondrial respiratory capacity and myosin heavy chain composition in repeated muscle biopsies. / Sahl, Ronni Eg; Morville, Thomas; Kraunsøe, Regitze; Dela, Flemming; Helge, Jørn Wulff; Larsen, Steen.

I: Analytical Biochemistry, Bind 556, 01.09.2018, s. 119-124.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Sahl, RE, Morville, T, Kraunsøe, R, Dela, F, Helge, JW & Larsen, S 2018, 'Variation in mitochondrial respiratory capacity and myosin heavy chain composition in repeated muscle biopsies', Analytical Biochemistry, bind 556, s. 119-124. https://doi.org/10.1016/j.ab.2018.06.029

APA

Sahl, R. E., Morville, T., Kraunsøe, R., Dela, F., Helge, J. W., & Larsen, S. (2018). Variation in mitochondrial respiratory capacity and myosin heavy chain composition in repeated muscle biopsies. Analytical Biochemistry, 556, 119-124. https://doi.org/10.1016/j.ab.2018.06.029

Vancouver

Sahl RE, Morville T, Kraunsøe R, Dela F, Helge JW, Larsen S. Variation in mitochondrial respiratory capacity and myosin heavy chain composition in repeated muscle biopsies. Analytical Biochemistry. 2018 sep 1;556:119-124. https://doi.org/10.1016/j.ab.2018.06.029

Author

Sahl, Ronni Eg ; Morville, Thomas ; Kraunsøe, Regitze ; Dela, Flemming ; Helge, Jørn Wulff ; Larsen, Steen. / Variation in mitochondrial respiratory capacity and myosin heavy chain composition in repeated muscle biopsies. I: Analytical Biochemistry. 2018 ; Bind 556. s. 119-124.

Bibtex

@article{ebbb85e234a64f62aa4233dcca40ec1b,
title = "Variation in mitochondrial respiratory capacity and myosin heavy chain composition in repeated muscle biopsies",
abstract = "Skeletal muscle is a heterogeneous tissue and it is essential to know the methodological variation and reliability when measuring aspects of muscle function. We assessed the methodological and biological variation when measuring mitochondrial respiratory capacity (MRC), citrate synthase (CS) activity and myosin heavy chain (MHC) composition in muscle biopsies from nine healthy male participants, and in addition we assessed variation in MRC in isolated mitochondria and yeast suspension. We analysed MRC, CS activity and MHC composition in duplicates (intra-biopsy variation) to quantify the methodological variation, as well as the biological variation from multiple muscle biopsies (inter-biopsy variation) obtained at different sites of the same muscle. Three muscle biopsies (B1, B2 and B3) were obtained from each subject in m. vastus lateralis. Two of the biopsies were from the same leg and one from the other leg. For MRC, intra-biopsy coefficient of variation (CV) was 8.4{\%} and inter-biopsy CV was 13.3{\%}. For MHC type I, IIa and IIx intra-biopsy CV was 8.3, 6.0 and 22.3{\%}, respectively. Inter-biopsy CV for these MHC types were 21.5, 15.4 and 42.0{\%}, respectively. For CS activity intra-biopsy CV was 0.6{\%} and inter-biopsy CV was 15.3{\%}. No differences between B1, B2 and B3 were detected for MRC, CS activity or MHC composition.",
author = "Sahl, {Ronni Eg} and Thomas Morville and Regitze Krauns{\o}e and Flemming Dela and Helge, {J{\o}rn Wulff} and Steen Larsen",
note = "Copyright {\circledC} 2018 Elsevier Inc. All rights reserved.",
year = "2018",
month = "9",
day = "1",
doi = "10.1016/j.ab.2018.06.029",
language = "English",
volume = "556",
pages = "119--124",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Variation in mitochondrial respiratory capacity and myosin heavy chain composition in repeated muscle biopsies

AU - Sahl, Ronni Eg

AU - Morville, Thomas

AU - Kraunsøe, Regitze

AU - Dela, Flemming

AU - Helge, Jørn Wulff

AU - Larsen, Steen

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Skeletal muscle is a heterogeneous tissue and it is essential to know the methodological variation and reliability when measuring aspects of muscle function. We assessed the methodological and biological variation when measuring mitochondrial respiratory capacity (MRC), citrate synthase (CS) activity and myosin heavy chain (MHC) composition in muscle biopsies from nine healthy male participants, and in addition we assessed variation in MRC in isolated mitochondria and yeast suspension. We analysed MRC, CS activity and MHC composition in duplicates (intra-biopsy variation) to quantify the methodological variation, as well as the biological variation from multiple muscle biopsies (inter-biopsy variation) obtained at different sites of the same muscle. Three muscle biopsies (B1, B2 and B3) were obtained from each subject in m. vastus lateralis. Two of the biopsies were from the same leg and one from the other leg. For MRC, intra-biopsy coefficient of variation (CV) was 8.4% and inter-biopsy CV was 13.3%. For MHC type I, IIa and IIx intra-biopsy CV was 8.3, 6.0 and 22.3%, respectively. Inter-biopsy CV for these MHC types were 21.5, 15.4 and 42.0%, respectively. For CS activity intra-biopsy CV was 0.6% and inter-biopsy CV was 15.3%. No differences between B1, B2 and B3 were detected for MRC, CS activity or MHC composition.

AB - Skeletal muscle is a heterogeneous tissue and it is essential to know the methodological variation and reliability when measuring aspects of muscle function. We assessed the methodological and biological variation when measuring mitochondrial respiratory capacity (MRC), citrate synthase (CS) activity and myosin heavy chain (MHC) composition in muscle biopsies from nine healthy male participants, and in addition we assessed variation in MRC in isolated mitochondria and yeast suspension. We analysed MRC, CS activity and MHC composition in duplicates (intra-biopsy variation) to quantify the methodological variation, as well as the biological variation from multiple muscle biopsies (inter-biopsy variation) obtained at different sites of the same muscle. Three muscle biopsies (B1, B2 and B3) were obtained from each subject in m. vastus lateralis. Two of the biopsies were from the same leg and one from the other leg. For MRC, intra-biopsy coefficient of variation (CV) was 8.4% and inter-biopsy CV was 13.3%. For MHC type I, IIa and IIx intra-biopsy CV was 8.3, 6.0 and 22.3%, respectively. Inter-biopsy CV for these MHC types were 21.5, 15.4 and 42.0%, respectively. For CS activity intra-biopsy CV was 0.6% and inter-biopsy CV was 15.3%. No differences between B1, B2 and B3 were detected for MRC, CS activity or MHC composition.

U2 - 10.1016/j.ab.2018.06.029

DO - 10.1016/j.ab.2018.06.029

M3 - Journal article

C2 - 29966588

VL - 556

SP - 119

EP - 124

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

ER -

ID: 203980815