The influence of age and cardiorespiratory fitness on bioactive lipids in muscle
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The influence of age and cardiorespiratory fitness on bioactive lipids in muscle. / Søgaard, Ditte; Baranowski, Marcin; Dela, Flemming; Helge, Jørn Wulff.
I: The journals of gerontology. Series A, Biological sciences and medical sciences, Bind 74, Nr. 6, 2019, s. 778-786.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The influence of age and cardiorespiratory fitness on bioactive lipids in muscle
AU - Søgaard, Ditte
AU - Baranowski, Marcin
AU - Dela, Flemming
AU - Helge, Jørn Wulff
PY - 2019
Y1 - 2019
N2 - Reduced insulin sensitivity is observed with aging and often explained by decreased physical activity. The mechanisms involved are not clarified, but bioactive lipids may play a role. We aimed to evaluate the influence of age and cardiorespiratory fitness on ceramide and diacylglycerol content in muscle and key proteins in lipid metabolism and insulin signaling.Healthy males were stratified by age into trained and untrained groups including 27 young (23.2±0.3 yrs.) and 33 aged (65.2±0.6 yrs.). Maximal oxygen uptake and body composition were measured and fasting blood samples and muscle biopsies obtained. Muscle ceramide and diacylglycerol were determined by thin-layer and gas-liquid chromatography and proteins by western blotting.We show that HOMA-IR was higher and VO2peak lower in aged compared to young. Total, saturated,C16:0 and C18:0 ceramide content were lower in muscle from aged compared to young. Intramuscular C18:1n9 and C20:4n6 content were higher in trained versus untrained. Content of total unsaturated and C16:1n7 diacylglycerol fatty acids were higher and C24:0 lower in muscle of aged versus young. Cardiorespiratory fitness had no impact on total diacylglycerol content. In conclusion, these data argue against intramuscular ceramide or diacylglycerol accumulation as driver of age-related insulin resistance in lean individuals.
AB - Reduced insulin sensitivity is observed with aging and often explained by decreased physical activity. The mechanisms involved are not clarified, but bioactive lipids may play a role. We aimed to evaluate the influence of age and cardiorespiratory fitness on ceramide and diacylglycerol content in muscle and key proteins in lipid metabolism and insulin signaling.Healthy males were stratified by age into trained and untrained groups including 27 young (23.2±0.3 yrs.) and 33 aged (65.2±0.6 yrs.). Maximal oxygen uptake and body composition were measured and fasting blood samples and muscle biopsies obtained. Muscle ceramide and diacylglycerol were determined by thin-layer and gas-liquid chromatography and proteins by western blotting.We show that HOMA-IR was higher and VO2peak lower in aged compared to young. Total, saturated,C16:0 and C18:0 ceramide content were lower in muscle from aged compared to young. Intramuscular C18:1n9 and C20:4n6 content were higher in trained versus untrained. Content of total unsaturated and C16:1n7 diacylglycerol fatty acids were higher and C24:0 lower in muscle of aged versus young. Cardiorespiratory fitness had no impact on total diacylglycerol content. In conclusion, these data argue against intramuscular ceramide or diacylglycerol accumulation as driver of age-related insulin resistance in lean individuals.
U2 - 10.1093/gerona/gly214
DO - 10.1093/gerona/gly214
M3 - Journal article
C2 - 30252030
VL - 74
SP - 778
EP - 786
JO - Journals of Gerontology. Series A: Biological Sciences & Medical Sciences
JF - Journals of Gerontology. Series A: Biological Sciences & Medical Sciences
SN - 1079-5006
IS - 6
ER -
ID: 203980520