The effect of age and unilateral leg immobilization for 2 weeks on substrate utilization during moderate intensity exercise in human skeletal muscle

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Standard

The effect of age and unilateral leg immobilization for 2 weeks on substrate utilization during moderate intensity exercise in human skeletal muscle. / Vigelsø, Andreas; Gram, Martin; Dybboe, Rie; Kuhlman, Anja Birk; Prats Gavalda, Clara; Greenhaff, P L; Constantin-Teodosiu, D; Birk, Jesper Bratz; Wojtaszewski, Jørgen; Dela, Flemming; Helge, Jørn Wulff.

I: Journal of Physiology, Bind 594, Nr. 8, 2016, s. 2339-2358.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Vigelsø, A, Gram, M, Dybboe, R, Kuhlman, AB, Prats Gavalda, C, Greenhaff, PL, Constantin-Teodosiu, D, Birk, JB, Wojtaszewski, J, Dela, F & Helge, JW 2016, 'The effect of age and unilateral leg immobilization for 2 weeks on substrate utilization during moderate intensity exercise in human skeletal muscle', Journal of Physiology, bind 594, nr. 8, s. 2339-2358. https://doi.org/10.1113/JP271712

APA

Vigelsø, A., Gram, M., Dybboe, R., Kuhlman, A. B., Prats Gavalda, C., Greenhaff, P. L., ... Helge, J. W. (2016). The effect of age and unilateral leg immobilization for 2 weeks on substrate utilization during moderate intensity exercise in human skeletal muscle. Journal of Physiology, 594(8), 2339-2358. https://doi.org/10.1113/JP271712

Vancouver

Vigelsø A, Gram M, Dybboe R, Kuhlman AB, Prats Gavalda C, Greenhaff PL o.a. The effect of age and unilateral leg immobilization for 2 weeks on substrate utilization during moderate intensity exercise in human skeletal muscle. Journal of Physiology. 2016;594(8):2339-2358. https://doi.org/10.1113/JP271712

Author

Vigelsø, Andreas ; Gram, Martin ; Dybboe, Rie ; Kuhlman, Anja Birk ; Prats Gavalda, Clara ; Greenhaff, P L ; Constantin-Teodosiu, D ; Birk, Jesper Bratz ; Wojtaszewski, Jørgen ; Dela, Flemming ; Helge, Jørn Wulff. / The effect of age and unilateral leg immobilization for 2 weeks on substrate utilization during moderate intensity exercise in human skeletal muscle. I: Journal of Physiology. 2016 ; Bind 594, Nr. 8. s. 2339-2358.

Bibtex

@article{57102fe6968d491db8fa70e9b44ad11e,
title = "The effect of age and unilateral leg immobilization for 2 weeks on substrate utilization during moderate intensity exercise in human skeletal muscle",
abstract = "Age and inactivity have been associated with intramuscular triglyceride (IMTG) accumulation. Here, we attempt to disentangle these factors by studying the effect of 2 weeks' unilateral leg immobilization on substrate utilization across the legs during moderate intensity exercise in young (n = 17; 23 ± 1 years) and older (n = 15; 68 ± 1 years) men, while the contralateral leg served as control. After immobilization, the participants performed two-legged isolated knee-extensor exercise at 20 ± 1 Watt (∼50{\%} Wattmax ) for 45 min with catheters inserted in the brachial artery and both femoral veins. Biopsy samples obtained from vastus lateralis muscles of both legs before and after exercise were used for analysis of substrates, protein content and enzyme activities. During exercise, leg substrate utilization (RQ) did not differ between groups or legs. Leg fatty acid (FA) uptake was greater in older than in young men, and while young men demonstrated net leg glycerol release during exercise, older men showed net glycerol uptake. At baseline, IMTG, muscle pyruvate dehydrogenase complex activity, protein content of adipose triglyceride lipase (ATGL), acetyl-CoA carboxylase 2, AMP-activated protein kinase (AMPK)γ3 were higher in young than in older men. Furthermore, ATGL, plasma membrane-associated FA binding protein, and AMPKγ3 subunit protein content were lower and IMTG being higher in the immobilized than the contralateral leg in young and older men. Thus, immobilization and age did not affect substrate choice (RQ) during moderate exercise, but the whole-leg and molecular differences in FA mobilization could explain the age and immobilization induced IMTG accumulation. This article is protected by copyright. All rights reserved.",
author = "Andreas Vigels{\o} and Martin Gram and Rie Dybboe and Kuhlman, {Anja Birk} and {Prats Gavalda}, Clara and Greenhaff, {P L} and D Constantin-Teodosiu and Birk, {Jesper Bratz} and J{\o}rgen Wojtaszewski and Flemming Dela and Helge, {J{\o}rn Wulff}",
note = "CURIS 2016 NEXS 092",
year = "2016",
doi = "10.1113/JP271712",
language = "English",
volume = "594",
pages = "2339--2358",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "8",

}

RIS

TY - JOUR

T1 - The effect of age and unilateral leg immobilization for 2 weeks on substrate utilization during moderate intensity exercise in human skeletal muscle

AU - Vigelsø, Andreas

AU - Gram, Martin

AU - Dybboe, Rie

AU - Kuhlman, Anja Birk

AU - Prats Gavalda, Clara

AU - Greenhaff, P L

AU - Constantin-Teodosiu, D

AU - Birk, Jesper Bratz

AU - Wojtaszewski, Jørgen

AU - Dela, Flemming

AU - Helge, Jørn Wulff

N1 - CURIS 2016 NEXS 092

PY - 2016

Y1 - 2016

N2 - Age and inactivity have been associated with intramuscular triglyceride (IMTG) accumulation. Here, we attempt to disentangle these factors by studying the effect of 2 weeks' unilateral leg immobilization on substrate utilization across the legs during moderate intensity exercise in young (n = 17; 23 ± 1 years) and older (n = 15; 68 ± 1 years) men, while the contralateral leg served as control. After immobilization, the participants performed two-legged isolated knee-extensor exercise at 20 ± 1 Watt (∼50% Wattmax ) for 45 min with catheters inserted in the brachial artery and both femoral veins. Biopsy samples obtained from vastus lateralis muscles of both legs before and after exercise were used for analysis of substrates, protein content and enzyme activities. During exercise, leg substrate utilization (RQ) did not differ between groups or legs. Leg fatty acid (FA) uptake was greater in older than in young men, and while young men demonstrated net leg glycerol release during exercise, older men showed net glycerol uptake. At baseline, IMTG, muscle pyruvate dehydrogenase complex activity, protein content of adipose triglyceride lipase (ATGL), acetyl-CoA carboxylase 2, AMP-activated protein kinase (AMPK)γ3 were higher in young than in older men. Furthermore, ATGL, plasma membrane-associated FA binding protein, and AMPKγ3 subunit protein content were lower and IMTG being higher in the immobilized than the contralateral leg in young and older men. Thus, immobilization and age did not affect substrate choice (RQ) during moderate exercise, but the whole-leg and molecular differences in FA mobilization could explain the age and immobilization induced IMTG accumulation. This article is protected by copyright. All rights reserved.

AB - Age and inactivity have been associated with intramuscular triglyceride (IMTG) accumulation. Here, we attempt to disentangle these factors by studying the effect of 2 weeks' unilateral leg immobilization on substrate utilization across the legs during moderate intensity exercise in young (n = 17; 23 ± 1 years) and older (n = 15; 68 ± 1 years) men, while the contralateral leg served as control. After immobilization, the participants performed two-legged isolated knee-extensor exercise at 20 ± 1 Watt (∼50% Wattmax ) for 45 min with catheters inserted in the brachial artery and both femoral veins. Biopsy samples obtained from vastus lateralis muscles of both legs before and after exercise were used for analysis of substrates, protein content and enzyme activities. During exercise, leg substrate utilization (RQ) did not differ between groups or legs. Leg fatty acid (FA) uptake was greater in older than in young men, and while young men demonstrated net leg glycerol release during exercise, older men showed net glycerol uptake. At baseline, IMTG, muscle pyruvate dehydrogenase complex activity, protein content of adipose triglyceride lipase (ATGL), acetyl-CoA carboxylase 2, AMP-activated protein kinase (AMPK)γ3 were higher in young than in older men. Furthermore, ATGL, plasma membrane-associated FA binding protein, and AMPKγ3 subunit protein content were lower and IMTG being higher in the immobilized than the contralateral leg in young and older men. Thus, immobilization and age did not affect substrate choice (RQ) during moderate exercise, but the whole-leg and molecular differences in FA mobilization could explain the age and immobilization induced IMTG accumulation. This article is protected by copyright. All rights reserved.

U2 - 10.1113/JP271712

DO - 10.1113/JP271712

M3 - Journal article

C2 - 26801521

VL - 594

SP - 2339

EP - 2358

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 8

ER -

ID: 154145475