T-cells and macrophages peak weeks after experimental stroke: Spatial and temporal characteristics

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

T-cells and macrophages peak weeks after experimental stroke : Spatial and temporal characteristics. / Vindegaard, Nina; Muñoz-Briones, Clara; El Ali, Henrik H.; Kristensen, Lotte Kellemann; Rasmussen, Rune Skovgaard; Johansen, Flemming Fryd; Hasseldam, Henrik.

I: Neuropathology, Bind 37, Nr. 5, 01.10.2017, s. 407-414.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Vindegaard, N, Muñoz-Briones, C, El Ali, HH, Kristensen, LK, Rasmussen, RS, Johansen, FF & Hasseldam, H 2017, 'T-cells and macrophages peak weeks after experimental stroke: Spatial and temporal characteristics', Neuropathology, bind 37, nr. 5, s. 407-414. https://doi.org/10.1111/neup.12387

APA

Vindegaard, N., Muñoz-Briones, C., El Ali, H. H., Kristensen, L. K., Rasmussen, R. S., Johansen, F. F., & Hasseldam, H. (2017). T-cells and macrophages peak weeks after experimental stroke: Spatial and temporal characteristics. Neuropathology, 37(5), 407-414. https://doi.org/10.1111/neup.12387

Vancouver

Vindegaard N, Muñoz-Briones C, El Ali HH, Kristensen LK, Rasmussen RS, Johansen FF o.a. T-cells and macrophages peak weeks after experimental stroke: Spatial and temporal characteristics. Neuropathology. 2017 okt. 1;37(5):407-414. https://doi.org/10.1111/neup.12387

Author

Vindegaard, Nina ; Muñoz-Briones, Clara ; El Ali, Henrik H. ; Kristensen, Lotte Kellemann ; Rasmussen, Rune Skovgaard ; Johansen, Flemming Fryd ; Hasseldam, Henrik. / T-cells and macrophages peak weeks after experimental stroke : Spatial and temporal characteristics. I: Neuropathology. 2017 ; Bind 37, Nr. 5. s. 407-414.

Bibtex

@article{45a211f9dc1344efabe182fc9d332c76,
title = "T-cells and macrophages peak weeks after experimental stroke: Spatial and temporal characteristics",
abstract = "The activities of the central and peripheral immune systems impact neurological outcome after ischemic stroke. However, studies investigating the temporal profile of leukocyte infiltration, especially T-cell recruitment, are sparse. Our aim was to investigate leukocyte infiltration at different time points after experimental stroke in mice. Permanent middle cerebral artery occlusion was performed on 11 weeks old C57BL/6J mice, allowed to survive for 1, 3, 8, 14 or 28 days. In addition to infarct size measurements, detailed immunohistochemical analyses of T-cell and macrophage influx were performed. A recently introduced F-19 MR probe (V-sense), designed to track macrophages, was furthermore tested. Fourteen and 28 days after permanent middle cerebral artery occlusion a significant increase in CD3+ T-cells was found within the ipsilateral hemisphere compared to controls, especially within the infarct core and the corpus callosum. The number of CD68+ cells within the infarct core was significantly increased at days 8, 14 and 28. This temporal pattern was also seen in MRI. After experimental stroke within the infarcted cortex we found a delayed (day 14) infiltration of T-cells and macrophages. Furthermore, our data show that T-cells are present in higher numbers in the corpus callosum compared to the rest of the brain (except from the infarct core where they were highest).",
keywords = "infarction, inflammation, macrophages, MCAO, T-cells",
author = "Nina Vindegaard and Clara Mu{\~n}oz-Briones and {El Ali}, {Henrik H.} and Kristensen, {Lotte Kellemann} and Rasmussen, {Rune Skovgaard} and Johansen, {Flemming Fryd} and Henrik Hasseldam",
year = "2017",
month = oct,
day = "1",
doi = "10.1111/neup.12387",
language = "English",
volume = "37",
pages = "407--414",
journal = "Neuropathology",
issn = "0919-6544",
publisher = "Wiley-Blackwell Publishing Asia",
number = "5",

}

RIS

TY - JOUR

T1 - T-cells and macrophages peak weeks after experimental stroke

T2 - Spatial and temporal characteristics

AU - Vindegaard, Nina

AU - Muñoz-Briones, Clara

AU - El Ali, Henrik H.

AU - Kristensen, Lotte Kellemann

AU - Rasmussen, Rune Skovgaard

AU - Johansen, Flemming Fryd

AU - Hasseldam, Henrik

PY - 2017/10/1

Y1 - 2017/10/1

N2 - The activities of the central and peripheral immune systems impact neurological outcome after ischemic stroke. However, studies investigating the temporal profile of leukocyte infiltration, especially T-cell recruitment, are sparse. Our aim was to investigate leukocyte infiltration at different time points after experimental stroke in mice. Permanent middle cerebral artery occlusion was performed on 11 weeks old C57BL/6J mice, allowed to survive for 1, 3, 8, 14 or 28 days. In addition to infarct size measurements, detailed immunohistochemical analyses of T-cell and macrophage influx were performed. A recently introduced F-19 MR probe (V-sense), designed to track macrophages, was furthermore tested. Fourteen and 28 days after permanent middle cerebral artery occlusion a significant increase in CD3+ T-cells was found within the ipsilateral hemisphere compared to controls, especially within the infarct core and the corpus callosum. The number of CD68+ cells within the infarct core was significantly increased at days 8, 14 and 28. This temporal pattern was also seen in MRI. After experimental stroke within the infarcted cortex we found a delayed (day 14) infiltration of T-cells and macrophages. Furthermore, our data show that T-cells are present in higher numbers in the corpus callosum compared to the rest of the brain (except from the infarct core where they were highest).

AB - The activities of the central and peripheral immune systems impact neurological outcome after ischemic stroke. However, studies investigating the temporal profile of leukocyte infiltration, especially T-cell recruitment, are sparse. Our aim was to investigate leukocyte infiltration at different time points after experimental stroke in mice. Permanent middle cerebral artery occlusion was performed on 11 weeks old C57BL/6J mice, allowed to survive for 1, 3, 8, 14 or 28 days. In addition to infarct size measurements, detailed immunohistochemical analyses of T-cell and macrophage influx were performed. A recently introduced F-19 MR probe (V-sense), designed to track macrophages, was furthermore tested. Fourteen and 28 days after permanent middle cerebral artery occlusion a significant increase in CD3+ T-cells was found within the ipsilateral hemisphere compared to controls, especially within the infarct core and the corpus callosum. The number of CD68+ cells within the infarct core was significantly increased at days 8, 14 and 28. This temporal pattern was also seen in MRI. After experimental stroke within the infarcted cortex we found a delayed (day 14) infiltration of T-cells and macrophages. Furthermore, our data show that T-cells are present in higher numbers in the corpus callosum compared to the rest of the brain (except from the infarct core where they were highest).

KW - infarction

KW - inflammation

KW - macrophages

KW - MCAO

KW - T-cells

U2 - 10.1111/neup.12387

DO - 10.1111/neup.12387

M3 - Journal article

C2 - 28517732

AN - SCOPUS:85019898450

VL - 37

SP - 407

EP - 414

JO - Neuropathology

JF - Neuropathology

SN - 0919-6544

IS - 5

ER -

ID: 185408624