Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion

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Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion. / Saltiel, Monika Yosifova; Kuhre, Rune Ehrenreich; Christiansen, Charlotte Bayer; Eliasen, Rasmus; Conde-Frieboes, Kilian W. ; Rosenkilde, Mette Marie; Holst, Jens Juul.

I: Nutrients, Bind 9, Nr. 4, 418, 2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Saltiel, MY, Kuhre, RE, Christiansen, CB, Eliasen, R, Conde-Frieboes, KW, Rosenkilde, MM & Holst, JJ 2017, 'Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion', Nutrients, bind 9, nr. 4, 418. https://doi.org/10.3390/nu9040418

APA

Saltiel, M. Y., Kuhre, R. E., Christiansen, C. B., Eliasen, R., Conde-Frieboes, K. W., Rosenkilde, M. M., & Holst, J. J. (2017). Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion. Nutrients, 9(4), [418]. https://doi.org/10.3390/nu9040418

Vancouver

Saltiel MY, Kuhre RE, Christiansen CB, Eliasen R, Conde-Frieboes KW, Rosenkilde MM o.a. Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion. Nutrients. 2017;9(4). 418. https://doi.org/10.3390/nu9040418

Author

Saltiel, Monika Yosifova ; Kuhre, Rune Ehrenreich ; Christiansen, Charlotte Bayer ; Eliasen, Rasmus ; Conde-Frieboes, Kilian W. ; Rosenkilde, Mette Marie ; Holst, Jens Juul. / Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion. I: Nutrients. 2017 ; Bind 9, Nr. 4.

Bibtex

@article{9e2d71545ed74e9aa57dce7812532cb2,
title = "Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion",
abstract = "Glucose stimulates the secretion of the incretin hormones: glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic peptide (GIP). It is debated whether the sweet taste receptor (STR) triggers this secretion. We investigated the role of STR activation for glucose-stimulated incretin secretion from an isolated perfused rat small intestine and whether selective STR activation by artificial sweeteners stimulates secretion. Intra-luminal administration of the STR agonists, acesulfame K (3.85{\%} w/v), but not sucralose (1.25{\%} w/v) and stevioside (2.5{\%} w/v), stimulated GLP-1 secretion (acesulfame K: 31 ± 3 pmol/L vs. 21 ± 2 pmol/L, p < 0.05, n = 6). In contrast, intra-arterial administration of sucralose (10 mM) and stevioside (10 mM), but not acesulfame K, stimulated GLP-1 secretion (sucralose: 51 ± 6 pmol/L vs. 34 ± 4 pmol/L, p < 0.05; stevioside: 54 ± 6 pmol/L vs. 32 ± 2 pmol/L, p < 0.05, n = 6), while 0.1 mM and 1 mM sucralose did not affect the secretion. Luminal glucose (20{\%} w/v) doubled GLP-1 and GIP secretion, but basolateral STR inhibition by gurmarin (2.5 µg/mL) or the inhibition of the transient receptor potential cation channel 5 (TRPM5) by triphenylphosphine oxide (TPPO) (100 µM) did not attenuate the responses. In conclusion, STR activation does not drive GIP/GLP-1 secretion itself, nor does it have a role for glucose-stimulated GLP-1 or GIP secretion",
author = "Saltiel, {Monika Yosifova} and Kuhre, {Rune Ehrenreich} and Christiansen, {Charlotte Bayer} and Rasmus Eliasen and Conde-Frieboes, {Kilian W.} and Rosenkilde, {Mette Marie} and Holst, {Jens Juul}",
year = "2017",
doi = "10.3390/nu9040418",
language = "English",
volume = "9",
journal = "Nutrients",
issn = "2072-6643",
publisher = "M D P I AG",
number = "4",

}

RIS

TY - JOUR

T1 - Sweet Taste Receptor Activation in the Gut Is of Limited Importance for Glucose-Stimulated GLP-1 and GIP Secretion

AU - Saltiel, Monika Yosifova

AU - Kuhre, Rune Ehrenreich

AU - Christiansen, Charlotte Bayer

AU - Eliasen, Rasmus

AU - Conde-Frieboes, Kilian W.

AU - Rosenkilde, Mette Marie

AU - Holst, Jens Juul

PY - 2017

Y1 - 2017

N2 - Glucose stimulates the secretion of the incretin hormones: glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic peptide (GIP). It is debated whether the sweet taste receptor (STR) triggers this secretion. We investigated the role of STR activation for glucose-stimulated incretin secretion from an isolated perfused rat small intestine and whether selective STR activation by artificial sweeteners stimulates secretion. Intra-luminal administration of the STR agonists, acesulfame K (3.85% w/v), but not sucralose (1.25% w/v) and stevioside (2.5% w/v), stimulated GLP-1 secretion (acesulfame K: 31 ± 3 pmol/L vs. 21 ± 2 pmol/L, p < 0.05, n = 6). In contrast, intra-arterial administration of sucralose (10 mM) and stevioside (10 mM), but not acesulfame K, stimulated GLP-1 secretion (sucralose: 51 ± 6 pmol/L vs. 34 ± 4 pmol/L, p < 0.05; stevioside: 54 ± 6 pmol/L vs. 32 ± 2 pmol/L, p < 0.05, n = 6), while 0.1 mM and 1 mM sucralose did not affect the secretion. Luminal glucose (20% w/v) doubled GLP-1 and GIP secretion, but basolateral STR inhibition by gurmarin (2.5 µg/mL) or the inhibition of the transient receptor potential cation channel 5 (TRPM5) by triphenylphosphine oxide (TPPO) (100 µM) did not attenuate the responses. In conclusion, STR activation does not drive GIP/GLP-1 secretion itself, nor does it have a role for glucose-stimulated GLP-1 or GIP secretion

AB - Glucose stimulates the secretion of the incretin hormones: glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic peptide (GIP). It is debated whether the sweet taste receptor (STR) triggers this secretion. We investigated the role of STR activation for glucose-stimulated incretin secretion from an isolated perfused rat small intestine and whether selective STR activation by artificial sweeteners stimulates secretion. Intra-luminal administration of the STR agonists, acesulfame K (3.85% w/v), but not sucralose (1.25% w/v) and stevioside (2.5% w/v), stimulated GLP-1 secretion (acesulfame K: 31 ± 3 pmol/L vs. 21 ± 2 pmol/L, p < 0.05, n = 6). In contrast, intra-arterial administration of sucralose (10 mM) and stevioside (10 mM), but not acesulfame K, stimulated GLP-1 secretion (sucralose: 51 ± 6 pmol/L vs. 34 ± 4 pmol/L, p < 0.05; stevioside: 54 ± 6 pmol/L vs. 32 ± 2 pmol/L, p < 0.05, n = 6), while 0.1 mM and 1 mM sucralose did not affect the secretion. Luminal glucose (20% w/v) doubled GLP-1 and GIP secretion, but basolateral STR inhibition by gurmarin (2.5 µg/mL) or the inhibition of the transient receptor potential cation channel 5 (TRPM5) by triphenylphosphine oxide (TPPO) (100 µM) did not attenuate the responses. In conclusion, STR activation does not drive GIP/GLP-1 secretion itself, nor does it have a role for glucose-stimulated GLP-1 or GIP secretion

U2 - 10.3390/nu9040418

DO - 10.3390/nu9040418

M3 - Journal article

VL - 9

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 4

M1 - 418

ER -

ID: 176887910