Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion

Publikation: Bidrag til bog/antologi/rapportKonferencebidrag i proceedingsForskningfagfællebedømt

Standard

Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion. / Andersen, Sofie Bech; Hoyos, Carlos Armando Villagomez; Taghavi, Iman; Gran, Fredrik; Hansen, Kristoffer Lindskov; Sorensen, Charlotte Mehlin; Jensen, Jorgen Arendt; Nielsen, Michael Bachmann.

2019 IEEE International Ultrasonics Symposium, IUS 2019. IEEE, 2019. s. 1169-1172 8926190 (IEEE International Ultrasonics Symposium, IUS, Bind 2019-October).

Publikation: Bidrag til bog/antologi/rapportKonferencebidrag i proceedingsForskningfagfællebedømt

Harvard

Andersen, SB, Hoyos, CAV, Taghavi, I, Gran, F, Hansen, KL, Sorensen, CM, Jensen, JA & Nielsen, MB 2019, Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion. i 2019 IEEE International Ultrasonics Symposium, IUS 2019., 8926190, IEEE, IEEE International Ultrasonics Symposium, IUS, bind 2019-October, s. 1169-1172, 2019 IEEE International Ultrasonics Symposium, IUS 2019, Glasgow, Storbritannien, 06/10/2019. https://doi.org/10.1109/ULTSYM.2019.8926190

APA

Andersen, S. B., Hoyos, C. A. V., Taghavi, I., Gran, F., Hansen, K. L., Sorensen, C. M., ... Nielsen, M. B. (2019). Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion. I 2019 IEEE International Ultrasonics Symposium, IUS 2019 (s. 1169-1172). [8926190] IEEE. IEEE International Ultrasonics Symposium, IUS, Bind. 2019-October https://doi.org/10.1109/ULTSYM.2019.8926190

Vancouver

Andersen SB, Hoyos CAV, Taghavi I, Gran F, Hansen KL, Sorensen CM o.a. Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion. I 2019 IEEE International Ultrasonics Symposium, IUS 2019. IEEE. 2019. s. 1169-1172. 8926190. (IEEE International Ultrasonics Symposium, IUS, Bind 2019-October). https://doi.org/10.1109/ULTSYM.2019.8926190

Author

Andersen, Sofie Bech ; Hoyos, Carlos Armando Villagomez ; Taghavi, Iman ; Gran, Fredrik ; Hansen, Kristoffer Lindskov ; Sorensen, Charlotte Mehlin ; Jensen, Jorgen Arendt ; Nielsen, Michael Bachmann. / Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion. 2019 IEEE International Ultrasonics Symposium, IUS 2019. IEEE, 2019. s. 1169-1172 (IEEE International Ultrasonics Symposium, IUS, Bind 2019-October).

Bibtex

@inproceedings{7fde2bf4e01a4759946c7600da9b4ab7,
title = "Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion",
abstract = "In vivo visualization of the microvasculature is feasible with super-resolution ultrasound imaging (SRI), but the method needs more affirmative data before clinical use. The kidneys have a rich vasculature, and microvascular dysfunction decreases the kidney function. Therefore, detection of subtle renal microvascular changes could benefit patients with renal disease. We hypothesized that our SRI setup can visualize the microvascular network of two healthy rat kidneys and subsequently demonstrate microvascular flow changes immediately after ischemia- reperfusion. The left kidney of two male Sprague-Dawley rats was scanned during laparotomy using a customized BK5000 scanner, an X18L5s transducer, and interleaved contrast and B-mode sequences with focused beam transmission. Images were acquired over 10 min using SonoVue (1:10) as the contrast agent. After a baseline scan, one rat had the renal vein clamped, while the other rat had the renal artery clamped, both for 45 min. The kidneys were rescanned immediately after clamp release and after 60 min of reperfusion. Motion correction was applied before microbubble (MB) detection. The characteristic renal microvascular structure was visualized with anatomical distinction between the dense cortical vascular network and the straight vessels of the medulla. Immediately after vein clamp release, almost no MBs perfused the medullary vessels. After 60 min of reperfusion, MBs refilled the renal vascular bed, but with a slower velocity compared with the baseline scan. The results after artery clamping were subtler, with more MBs perfusing the medulla immediately after clamp release compared with the vein clamping. The results imply that this SRI setup can evaluate different stages of ischemic kidney disease in rats as it can visualize the entire renal vascular bed and differentiate the pattern of reperfusion in two types of ischemic injury.",
author = "Andersen, {Sofie Bech} and Hoyos, {Carlos Armando Villagomez} and Iman Taghavi and Fredrik Gran and Hansen, {Kristoffer Lindskov} and Sorensen, {Charlotte Mehlin} and Jensen, {Jorgen Arendt} and Nielsen, {Michael Bachmann}",
year = "2019",
doi = "10.1109/ULTSYM.2019.8926190",
language = "English",
series = "IEEE International Ultrasonics Symposium, IUS",
publisher = "IEEE",
pages = "1169--1172",
booktitle = "2019 IEEE International Ultrasonics Symposium, IUS 2019",
note = "2019 IEEE International Ultrasonics Symposium, IUS 2019 ; Conference date: 06-10-2019 Through 09-10-2019",

}

RIS

TY - GEN

T1 - Super-Resolution Ultrasound Imaging of Rat Kidneys before and after Ischemia-Reperfusion

AU - Andersen, Sofie Bech

AU - Hoyos, Carlos Armando Villagomez

AU - Taghavi, Iman

AU - Gran, Fredrik

AU - Hansen, Kristoffer Lindskov

AU - Sorensen, Charlotte Mehlin

AU - Jensen, Jorgen Arendt

AU - Nielsen, Michael Bachmann

PY - 2019

Y1 - 2019

N2 - In vivo visualization of the microvasculature is feasible with super-resolution ultrasound imaging (SRI), but the method needs more affirmative data before clinical use. The kidneys have a rich vasculature, and microvascular dysfunction decreases the kidney function. Therefore, detection of subtle renal microvascular changes could benefit patients with renal disease. We hypothesized that our SRI setup can visualize the microvascular network of two healthy rat kidneys and subsequently demonstrate microvascular flow changes immediately after ischemia- reperfusion. The left kidney of two male Sprague-Dawley rats was scanned during laparotomy using a customized BK5000 scanner, an X18L5s transducer, and interleaved contrast and B-mode sequences with focused beam transmission. Images were acquired over 10 min using SonoVue (1:10) as the contrast agent. After a baseline scan, one rat had the renal vein clamped, while the other rat had the renal artery clamped, both for 45 min. The kidneys were rescanned immediately after clamp release and after 60 min of reperfusion. Motion correction was applied before microbubble (MB) detection. The characteristic renal microvascular structure was visualized with anatomical distinction between the dense cortical vascular network and the straight vessels of the medulla. Immediately after vein clamp release, almost no MBs perfused the medullary vessels. After 60 min of reperfusion, MBs refilled the renal vascular bed, but with a slower velocity compared with the baseline scan. The results after artery clamping were subtler, with more MBs perfusing the medulla immediately after clamp release compared with the vein clamping. The results imply that this SRI setup can evaluate different stages of ischemic kidney disease in rats as it can visualize the entire renal vascular bed and differentiate the pattern of reperfusion in two types of ischemic injury.

AB - In vivo visualization of the microvasculature is feasible with super-resolution ultrasound imaging (SRI), but the method needs more affirmative data before clinical use. The kidneys have a rich vasculature, and microvascular dysfunction decreases the kidney function. Therefore, detection of subtle renal microvascular changes could benefit patients with renal disease. We hypothesized that our SRI setup can visualize the microvascular network of two healthy rat kidneys and subsequently demonstrate microvascular flow changes immediately after ischemia- reperfusion. The left kidney of two male Sprague-Dawley rats was scanned during laparotomy using a customized BK5000 scanner, an X18L5s transducer, and interleaved contrast and B-mode sequences with focused beam transmission. Images were acquired over 10 min using SonoVue (1:10) as the contrast agent. After a baseline scan, one rat had the renal vein clamped, while the other rat had the renal artery clamped, both for 45 min. The kidneys were rescanned immediately after clamp release and after 60 min of reperfusion. Motion correction was applied before microbubble (MB) detection. The characteristic renal microvascular structure was visualized with anatomical distinction between the dense cortical vascular network and the straight vessels of the medulla. Immediately after vein clamp release, almost no MBs perfused the medullary vessels. After 60 min of reperfusion, MBs refilled the renal vascular bed, but with a slower velocity compared with the baseline scan. The results after artery clamping were subtler, with more MBs perfusing the medulla immediately after clamp release compared with the vein clamping. The results imply that this SRI setup can evaluate different stages of ischemic kidney disease in rats as it can visualize the entire renal vascular bed and differentiate the pattern of reperfusion in two types of ischemic injury.

U2 - 10.1109/ULTSYM.2019.8926190

DO - 10.1109/ULTSYM.2019.8926190

M3 - Article in proceedings

AN - SCOPUS:85077578398

T3 - IEEE International Ultrasonics Symposium, IUS

SP - 1169

EP - 1172

BT - 2019 IEEE International Ultrasonics Symposium, IUS 2019

PB - IEEE

T2 - 2019 IEEE International Ultrasonics Symposium, IUS 2019

Y2 - 6 October 2019 through 9 October 2019

ER -

ID: 234446618