Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures

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Standard

Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures. / Tanner, V A; Ploug, Thorkil; Tao-Cheng, J H.

I: Journal of Histochemistry and Cytochemistry, Bind 44, Nr. 12, 12.1996, s. 1481-8.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Tanner, VA, Ploug, T & Tao-Cheng, JH 1996, 'Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures', Journal of Histochemistry and Cytochemistry, bind 44, nr. 12, s. 1481-8.

APA

Tanner, V. A., Ploug, T., & Tao-Cheng, J. H. (1996). Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures. Journal of Histochemistry and Cytochemistry, 44(12), 1481-8.

Vancouver

Tanner VA, Ploug T, Tao-Cheng JH. Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures. Journal of Histochemistry and Cytochemistry. 1996 dec;44(12):1481-8.

Author

Tanner, V A ; Ploug, Thorkil ; Tao-Cheng, J H. / Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures. I: Journal of Histochemistry and Cytochemistry. 1996 ; Bind 44, Nr. 12. s. 1481-8.

Bibtex

@article{550182fcdbc443a799c0e72bccef9e2d,
title = "Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures",
abstract = "We demonstrated the subcellular localization of SV2, a transmembrane protein associated with neuroendocrine secretory vesicles, in NGF-treated PC12 cells by preembedding EM immunocytochemistry (ICC), using a small gold probe followed by silver enhancement. The use of a multiwell chamber slide substantially improved the efficiency of the preembedding EM ICC procedures for cell cultures. The advantages and related caveats of this method are discussed. SV2 was distinctly localized on dusters of synaptic vesicles and large dense-cored vesicles (LDCV). The distribution of SV2 on these two types of secretory vesicles was compared quantitatively to that of another secretory vesicle-associated transmembrane protein, synaptophysin. In cultures under similar experimental conditions, the ratio of SV2 vs synaptophysin ICC staining on synaptic vesicle dusters was about 1:1, whereas it was about 9:1 on LDCV membranes. Furthermore, whereas SV2 is localized on the membranes of the LDCVs, chromogranin A, an acidic protein in secretory granules, is clearly in the core of the LDCVs. This is the first demonstration of these two antigens in such dose (approximately 20 nm) yet distinct compartments within a single organelle.",
keywords = "Animals, Cells, Cultured, Immunohistochemistry, Membrane Glycoproteins, Microscopy, Electron, Nerve Tissue Proteins, PC12 Cells, Rats, Subcellular Fractions, Synaptic Vesicles, Synaptophysin",
author = "Tanner, {V A} and Thorkil Ploug and Tao-Cheng, {J H}",
year = "1996",
month = "12",
language = "English",
volume = "44",
pages = "1481--8",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "SAGE Publications",
number = "12",

}

RIS

TY - JOUR

T1 - Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures

AU - Tanner, V A

AU - Ploug, Thorkil

AU - Tao-Cheng, J H

PY - 1996/12

Y1 - 1996/12

N2 - We demonstrated the subcellular localization of SV2, a transmembrane protein associated with neuroendocrine secretory vesicles, in NGF-treated PC12 cells by preembedding EM immunocytochemistry (ICC), using a small gold probe followed by silver enhancement. The use of a multiwell chamber slide substantially improved the efficiency of the preembedding EM ICC procedures for cell cultures. The advantages and related caveats of this method are discussed. SV2 was distinctly localized on dusters of synaptic vesicles and large dense-cored vesicles (LDCV). The distribution of SV2 on these two types of secretory vesicles was compared quantitatively to that of another secretory vesicle-associated transmembrane protein, synaptophysin. In cultures under similar experimental conditions, the ratio of SV2 vs synaptophysin ICC staining on synaptic vesicle dusters was about 1:1, whereas it was about 9:1 on LDCV membranes. Furthermore, whereas SV2 is localized on the membranes of the LDCVs, chromogranin A, an acidic protein in secretory granules, is clearly in the core of the LDCVs. This is the first demonstration of these two antigens in such dose (approximately 20 nm) yet distinct compartments within a single organelle.

AB - We demonstrated the subcellular localization of SV2, a transmembrane protein associated with neuroendocrine secretory vesicles, in NGF-treated PC12 cells by preembedding EM immunocytochemistry (ICC), using a small gold probe followed by silver enhancement. The use of a multiwell chamber slide substantially improved the efficiency of the preembedding EM ICC procedures for cell cultures. The advantages and related caveats of this method are discussed. SV2 was distinctly localized on dusters of synaptic vesicles and large dense-cored vesicles (LDCV). The distribution of SV2 on these two types of secretory vesicles was compared quantitatively to that of another secretory vesicle-associated transmembrane protein, synaptophysin. In cultures under similar experimental conditions, the ratio of SV2 vs synaptophysin ICC staining on synaptic vesicle dusters was about 1:1, whereas it was about 9:1 on LDCV membranes. Furthermore, whereas SV2 is localized on the membranes of the LDCVs, chromogranin A, an acidic protein in secretory granules, is clearly in the core of the LDCVs. This is the first demonstration of these two antigens in such dose (approximately 20 nm) yet distinct compartments within a single organelle.

KW - Animals

KW - Cells, Cultured

KW - Immunohistochemistry

KW - Membrane Glycoproteins

KW - Microscopy, Electron

KW - Nerve Tissue Proteins

KW - PC12 Cells

KW - Rats

KW - Subcellular Fractions

KW - Synaptic Vesicles

KW - Synaptophysin

M3 - Journal article

C2 - 8985140

VL - 44

SP - 1481

EP - 1488

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 12

ER -

ID: 123666069