Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture

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Standard

Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture. / Eff, C; Deckert, T; Andersson, A; Nielsen, Jens Høiriis.

I: Diabetologia, Bind 20, Nr. 1, 1981, s. 60-5.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Eff, C, Deckert, T, Andersson, A & Nielsen, JH 1981, 'Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture', Diabetologia, bind 20, nr. 1, s. 60-5.

APA

Eff, C., Deckert, T., Andersson, A., & Nielsen, J. H. (1981). Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture. Diabetologia, 20(1), 60-5.

Vancouver

Eff C, Deckert T, Andersson A, Nielsen JH. Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture. Diabetologia. 1981;20(1):60-5.

Author

Eff, C ; Deckert, T ; Andersson, A ; Nielsen, Jens Høiriis. / Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture. I: Diabetologia. 1981 ; Bind 20, Nr. 1. s. 60-5.

Bibtex

@article{ef97278ff8944ac49a76780aa39d34ce,
title = "Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture",
abstract = "Islets of Langerhans from NMRI-mice were kept for one week in tissue culture in medium supplemented with human serum obtained from either normal healthy subjects or newly diagnosed juvenile diabetic patients before insulin treatment. Islets cultured in diabetic serum released more insulin than islets cultured in normal serum, whether tissue culture medium 199 with 5.5-8.3 mmol/l glucose and 10{\%} serum, or culture medium RPMI 1640 with 11 mmol/l glucose and 0.5{\%} serum were used. Islets kept for one week in culture with diabetic serum did not show any decrease in DNA content or glucose induced insulin secretion and biosynthesis. It is concluded that serum from newly diagnosed insulin-dependent diabetic patients stimulates insulin release from isolated mouse islets kept in tissue culture. The underlying mechanism is unknown.",
keywords = "Animals, Culture Media, Culture Techniques, Diabetes Mellitus, Female, Humans, Insulin, Islets of Langerhans, Male, Mice",
author = "C Eff and T Deckert and A Andersson and Nielsen, {Jens H{\o}iriis}",
year = "1981",
language = "English",
volume = "20",
pages = "60--5",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture

AU - Eff, C

AU - Deckert, T

AU - Andersson, A

AU - Nielsen, Jens Høiriis

PY - 1981

Y1 - 1981

N2 - Islets of Langerhans from NMRI-mice were kept for one week in tissue culture in medium supplemented with human serum obtained from either normal healthy subjects or newly diagnosed juvenile diabetic patients before insulin treatment. Islets cultured in diabetic serum released more insulin than islets cultured in normal serum, whether tissue culture medium 199 with 5.5-8.3 mmol/l glucose and 10% serum, or culture medium RPMI 1640 with 11 mmol/l glucose and 0.5% serum were used. Islets kept for one week in culture with diabetic serum did not show any decrease in DNA content or glucose induced insulin secretion and biosynthesis. It is concluded that serum from newly diagnosed insulin-dependent diabetic patients stimulates insulin release from isolated mouse islets kept in tissue culture. The underlying mechanism is unknown.

AB - Islets of Langerhans from NMRI-mice were kept for one week in tissue culture in medium supplemented with human serum obtained from either normal healthy subjects or newly diagnosed juvenile diabetic patients before insulin treatment. Islets cultured in diabetic serum released more insulin than islets cultured in normal serum, whether tissue culture medium 199 with 5.5-8.3 mmol/l glucose and 10% serum, or culture medium RPMI 1640 with 11 mmol/l glucose and 0.5% serum were used. Islets kept for one week in culture with diabetic serum did not show any decrease in DNA content or glucose induced insulin secretion and biosynthesis. It is concluded that serum from newly diagnosed insulin-dependent diabetic patients stimulates insulin release from isolated mouse islets kept in tissue culture. The underlying mechanism is unknown.

KW - Animals

KW - Culture Media

KW - Culture Techniques

KW - Diabetes Mellitus

KW - Female

KW - Humans

KW - Insulin

KW - Islets of Langerhans

KW - Male

KW - Mice

M3 - Journal article

VL - 20

SP - 60

EP - 65

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 1

ER -

ID: 47975470