Stimulation history affects vasomotor responses in rat mesenteric arterioles

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Standard

Stimulation history affects vasomotor responses in rat mesenteric arterioles. / Hald, Bjørn Olav; Sørensen, Rasmus B.; Sørensen, Preben Graae; Sørensen, Charlotte Mehlin; Jacobsen, Jens Christian Brings.

I: Pflügers Archiv - European Journal of Physiology, Bind 471, Nr. 2, 2019, s. 271-283.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Hald, BO, Sørensen, RB, Sørensen, PG, Sørensen, CM & Jacobsen, JCB 2019, 'Stimulation history affects vasomotor responses in rat mesenteric arterioles', Pflügers Archiv - European Journal of Physiology, bind 471, nr. 2, s. 271-283. https://doi.org/10.1007/s00424-018-2206-0

APA

Hald, B. O., Sørensen, R. B., Sørensen, P. G., Sørensen, C. M., & Jacobsen, J. C. B. (2019). Stimulation history affects vasomotor responses in rat mesenteric arterioles. Pflügers Archiv - European Journal of Physiology, 471(2), 271-283. https://doi.org/10.1007/s00424-018-2206-0

Vancouver

Hald BO, Sørensen RB, Sørensen PG, Sørensen CM, Jacobsen JCB. Stimulation history affects vasomotor responses in rat mesenteric arterioles. Pflügers Archiv - European Journal of Physiology. 2019;471(2):271-283. https://doi.org/10.1007/s00424-018-2206-0

Author

Hald, Bjørn Olav ; Sørensen, Rasmus B. ; Sørensen, Preben Graae ; Sørensen, Charlotte Mehlin ; Jacobsen, Jens Christian Brings. / Stimulation history affects vasomotor responses in rat mesenteric arterioles. I: Pflügers Archiv - European Journal of Physiology. 2019 ; Bind 471, Nr. 2. s. 271-283.

Bibtex

@article{c00d8296785f4a3e972aeafb3007e966,
title = "Stimulation history affects vasomotor responses in rat mesenteric arterioles",
abstract = "Resistance vessels regulate blood flow by continuously adjusting activity of the wall smooth muscle cells. These cells integrate a variety of stimuli from blood, endothelium, autonomic nerves, and surrounding tissues. Each stimulus elicits an intracellular signaling cascade that eventually influences activation of the contractile machinery. The characteristic time scale of each cascade and the sharing of specific reactions between cascades provide for complex behavior when a vessel receives multiple stimuli. Here, we apply sequential stimulation with invariant concentrations of vasoconstrictor (norepinephrine/methoxamine) and vasodilator (SNAP/carbacol) to rat mesenteric vessels in the wire myograph to show that (1) time elapsed between addition of two vasoactive drugs and (2) the sequence of addition may significantly affect final force development. Furthermore, force oscillations (vasomotion) often appear upon norepinephrine administration. Using computational modeling in combination with nitric oxide (NO) inhibition/NO addition experiments, we show that (3) amplitude and number of oscillating vessels increase over time, (4) the ability of NO to induce vasomotion depends on whether it is applied before or after norepinephrine, and (5) emergence of vasomotion depends on the prior dynamical state of the system; in simulations, this phenomenon appears as {"}hysteresis.{"} These findings underscore the time-dependent nature of vascular tone generation which must be considered when evaluating the vasomotor effects of multiple, simultaneous stimuli in vitro or in vivo.",
keywords = "Contractile state, Mesenteric artery, Non-linear dynamics, Vasomotion",
author = "Hald, {Bj{\o}rn Olav} and S{\o}rensen, {Rasmus B.} and S{\o}rensen, {Preben Graae} and S{\o}rensen, {Charlotte Mehlin} and Jacobsen, {Jens Christian Brings}",
year = "2019",
doi = "10.1007/s00424-018-2206-0",
language = "English",
volume = "471",
pages = "271--283",
journal = "Pfl{\"u}gers Archiv - European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Stimulation history affects vasomotor responses in rat mesenteric arterioles

AU - Hald, Bjørn Olav

AU - Sørensen, Rasmus B.

AU - Sørensen, Preben Graae

AU - Sørensen, Charlotte Mehlin

AU - Jacobsen, Jens Christian Brings

PY - 2019

Y1 - 2019

N2 - Resistance vessels regulate blood flow by continuously adjusting activity of the wall smooth muscle cells. These cells integrate a variety of stimuli from blood, endothelium, autonomic nerves, and surrounding tissues. Each stimulus elicits an intracellular signaling cascade that eventually influences activation of the contractile machinery. The characteristic time scale of each cascade and the sharing of specific reactions between cascades provide for complex behavior when a vessel receives multiple stimuli. Here, we apply sequential stimulation with invariant concentrations of vasoconstrictor (norepinephrine/methoxamine) and vasodilator (SNAP/carbacol) to rat mesenteric vessels in the wire myograph to show that (1) time elapsed between addition of two vasoactive drugs and (2) the sequence of addition may significantly affect final force development. Furthermore, force oscillations (vasomotion) often appear upon norepinephrine administration. Using computational modeling in combination with nitric oxide (NO) inhibition/NO addition experiments, we show that (3) amplitude and number of oscillating vessels increase over time, (4) the ability of NO to induce vasomotion depends on whether it is applied before or after norepinephrine, and (5) emergence of vasomotion depends on the prior dynamical state of the system; in simulations, this phenomenon appears as "hysteresis." These findings underscore the time-dependent nature of vascular tone generation which must be considered when evaluating the vasomotor effects of multiple, simultaneous stimuli in vitro or in vivo.

AB - Resistance vessels regulate blood flow by continuously adjusting activity of the wall smooth muscle cells. These cells integrate a variety of stimuli from blood, endothelium, autonomic nerves, and surrounding tissues. Each stimulus elicits an intracellular signaling cascade that eventually influences activation of the contractile machinery. The characteristic time scale of each cascade and the sharing of specific reactions between cascades provide for complex behavior when a vessel receives multiple stimuli. Here, we apply sequential stimulation with invariant concentrations of vasoconstrictor (norepinephrine/methoxamine) and vasodilator (SNAP/carbacol) to rat mesenteric vessels in the wire myograph to show that (1) time elapsed between addition of two vasoactive drugs and (2) the sequence of addition may significantly affect final force development. Furthermore, force oscillations (vasomotion) often appear upon norepinephrine administration. Using computational modeling in combination with nitric oxide (NO) inhibition/NO addition experiments, we show that (3) amplitude and number of oscillating vessels increase over time, (4) the ability of NO to induce vasomotion depends on whether it is applied before or after norepinephrine, and (5) emergence of vasomotion depends on the prior dynamical state of the system; in simulations, this phenomenon appears as "hysteresis." These findings underscore the time-dependent nature of vascular tone generation which must be considered when evaluating the vasomotor effects of multiple, simultaneous stimuli in vitro or in vivo.

KW - Contractile state

KW - Mesenteric artery

KW - Non-linear dynamics

KW - Vasomotion

U2 - 10.1007/s00424-018-2206-0

DO - 10.1007/s00424-018-2206-0

M3 - Journal article

VL - 471

SP - 271

EP - 283

JO - Pflügers Archiv - European Journal of Physiology

JF - Pflügers Archiv - European Journal of Physiology

SN - 0031-6768

IS - 2

ER -

ID: 203861730