Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution

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Standard

Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution. / Martinez-Val, Ana; Bekker-Jensen, Dorte B.; Steigerwald, Sophia; Koenig, Claire; Østergaard, Ole; Mehta, Adi; Tran, Trung; Sikorski, Krzysztof; Torres-Vega, Estefanía; Kwasniewicz, Ewa; Brynjólfsdóttir, Sólveig Hlín; Frankel, Lisa B.; Kjøbsted, Rasmus; Krogh, Nicolai; Lundby, Alicia; Bekker-Jensen, Simon; Lund-Johansen, Fridtjof; Olsen, Jesper V.

I: Nature Communications, Bind 12, Nr. 1, 7113, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Martinez-Val, A, Bekker-Jensen, DB, Steigerwald, S, Koenig, C, Østergaard, O, Mehta, A, Tran, T, Sikorski, K, Torres-Vega, E, Kwasniewicz, E, Brynjólfsdóttir, SH, Frankel, LB, Kjøbsted, R, Krogh, N, Lundby, A, Bekker-Jensen, S, Lund-Johansen, F & Olsen, JV 2021, 'Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution', Nature Communications, bind 12, nr. 1, 7113. https://doi.org/10.1038/s41467-021-27398-y

APA

Martinez-Val, A., Bekker-Jensen, D. B., Steigerwald, S., Koenig, C., Østergaard, O., Mehta, A., Tran, T., Sikorski, K., Torres-Vega, E., Kwasniewicz, E., Brynjólfsdóttir, S. H., Frankel, L. B., Kjøbsted, R., Krogh, N., Lundby, A., Bekker-Jensen, S., Lund-Johansen, F., & Olsen, J. V. (2021). Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution. Nature Communications, 12(1), [7113]. https://doi.org/10.1038/s41467-021-27398-y

Vancouver

Martinez-Val A, Bekker-Jensen DB, Steigerwald S, Koenig C, Østergaard O, Mehta A o.a. Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution. Nature Communications. 2021;12(1). 7113. https://doi.org/10.1038/s41467-021-27398-y

Author

Martinez-Val, Ana ; Bekker-Jensen, Dorte B. ; Steigerwald, Sophia ; Koenig, Claire ; Østergaard, Ole ; Mehta, Adi ; Tran, Trung ; Sikorski, Krzysztof ; Torres-Vega, Estefanía ; Kwasniewicz, Ewa ; Brynjólfsdóttir, Sólveig Hlín ; Frankel, Lisa B. ; Kjøbsted, Rasmus ; Krogh, Nicolai ; Lundby, Alicia ; Bekker-Jensen, Simon ; Lund-Johansen, Fridtjof ; Olsen, Jesper V. / Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution. I: Nature Communications. 2021 ; Bind 12, Nr. 1.

Bibtex

@article{e065a1a6910241b3988d3b7b52f3cf82,

title = "Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution",

abstract = "Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of protein networks in cells, but involves laborious workflows that does not cover the phospho-proteome level. Here we present a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. We benchmark the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in vitro in HeLa cells and in vivo in mouse tissues. Finally, we investigate the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. Proteomics data generated in this study can be explored through https://SpatialProteoDynamics.github.io .",

author = "Ana Martinez-Val and Bekker-Jensen, {Dorte B.} and Sophia Steigerwald and Claire Koenig and Ole {\O}stergaard and Adi Mehta and Trung Tran and Krzysztof Sikorski and Estefan{\'i}a Torres-Vega and Ewa Kwasniewicz and Brynj{\'o}lfsd{\'o}ttir, {S{\'o}lveig Hl{\'i}n} and Frankel, {Lisa B.} and Rasmus Kj{\o}bsted and Nicolai Krogh and Alicia Lundby and Simon Bekker-Jensen and Fridtjof Lund-Johansen and Olsen, {Jesper V.}",

note = "{\textcopyright} 2021. The Author(s).",

year = "2021",

doi = "10.1038/s41467-021-27398-y",

language = "English",

volume = "12",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "nature publishing group",

number = "1",

}

RIS

TY - JOUR

T1 - Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution

AU - Martinez-Val, Ana

AU - Bekker-Jensen, Dorte B.

AU - Steigerwald, Sophia

AU - Koenig, Claire

AU - Østergaard, Ole

AU - Mehta, Adi

AU - Tran, Trung

AU - Sikorski, Krzysztof

AU - Torres-Vega, Estefanía

AU - Kwasniewicz, Ewa

AU - Brynjólfsdóttir, Sólveig Hlín

AU - Frankel, Lisa B.

AU - Kjøbsted, Rasmus

AU - Krogh, Nicolai

AU - Lundby, Alicia

AU - Bekker-Jensen, Simon

AU - Lund-Johansen, Fridtjof

AU - Olsen, Jesper V.

PY - 2021

Y1 - 2021

N2 - Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of protein networks in cells, but involves laborious workflows that does not cover the phospho-proteome level. Here we present a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. We benchmark the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in vitro in HeLa cells and in vivo in mouse tissues. Finally, we investigate the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. Proteomics data generated in this study can be explored through https://SpatialProteoDynamics.github.io .

AB - Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of protein networks in cells, but involves laborious workflows that does not cover the phospho-proteome level. Here we present a high-throughput workflow based on sequential cell fractionation to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. We benchmark the workflow by studying spatio-temporal EGFR phospho-signaling dynamics in vitro in HeLa cells and in vivo in mouse tissues. Finally, we investigate the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. Proteomics data generated in this study can be explored through https://SpatialProteoDynamics.github.io .

U2 - 10.1038/s41467-021-27398-y

DO - 10.1038/s41467-021-27398-y

M3 - Journal article

C2 - 34876567

VL - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 7113

ER -

ID: 286851106

Biomedicinsk Institut