Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma

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Standard

Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma. / Etzerodt, Anders; Berg, Ronan M G; Plovsing, Ronni R; Andersen, Morten N; Bebien, Magali; Habbeddine, Mohamed; Lawrence, Toby; Møller, Holger J.; Moestrup, Søren K.

I: Scientific Reports, Bind 7, 40286, 2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Etzerodt, A, Berg, RMG, Plovsing, RR, Andersen, MN, Bebien, M, Habbeddine, M, Lawrence, T, Møller, HJ & Moestrup, SK 2017, 'Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma', Scientific Reports, bind 7, 40286. https://doi.org/10.1038/srep40286

APA

Etzerodt, A., Berg, R. M. G., Plovsing, R. R., Andersen, M. N., Bebien, M., Habbeddine, M., Lawrence, T., Møller, H. J., & Moestrup, S. K. (2017). Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma. Scientific Reports, 7, [40286]. https://doi.org/10.1038/srep40286

Vancouver

Etzerodt A, Berg RMG, Plovsing RR, Andersen MN, Bebien M, Habbeddine M o.a. Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma. Scientific Reports. 2017;7. 40286. https://doi.org/10.1038/srep40286

Author

Etzerodt, Anders ; Berg, Ronan M G ; Plovsing, Ronni R ; Andersen, Morten N ; Bebien, Magali ; Habbeddine, Mohamed ; Lawrence, Toby ; Møller, Holger J. ; Moestrup, Søren K. / Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma. I: Scientific Reports. 2017 ; Bind 7.

Bibtex

@article{9e5c39ac480843a6a683ed49b082e246,
title = "Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma",
abstract = "CD163 is the macrophage receptor for uptake of hemoglobin-haptoglobin complexes. The human receptor can be shed from the macrophage surface owing to a cleavage site for the inflammation-inducible TACE/ADAM17 enzyme. Accordingly, plasma 'soluble CD163' (sCD163) has become a biomarker for macrophage activity and inflammation. The present study disclosed that 10% of sCD163 in healthy persons is actually extracellular vesicle (EV)-associated CD163 not being cleaved and shed. Endotoxin injection of human volunteers caused a selective increase in the ectodomain CD163, while septic patients exhibited high levels of both soluble ectodomain CD163 and extracellular vesicle (EV) CD163, the latter representing up 60% of total plasma CD163. A poor prognosis of septic patients measured as the sequential organ failure assessment (SOFA) score correlated with the increase in membrane-associated CD163. Our results show that soluble ectodomain CD163 and EV CD163 in plasma are part of separate macrophage response in the context of systemic inflammation. While that soluble ectodomain CD163 is released during the acute systemic inflammatory response, this is not the case for EV CD163 that instead may be released during a later phase of the inflammatory response. A separate measurement of the two forms of CD163 constituting 'soluble CD163' in plasma may therefore add to the diagnostic and prognostic value.",
keywords = "Adult, Aged, Antigens, CD/blood, Antigens, Differentiation, Myelomonocytic/blood, Biomarkers/blood, Endotoxins/administration & dosage, Extracellular Vesicles/chemistry, Female, Haptoglobins/chemistry, Healthy Volunteers, Hemoglobins/chemistry, Humans, Inflammation/blood, Macrophages/drug effects, Male, Middle Aged, Prognosis, Protein Isoforms/genetics, Receptors, Cell Surface/blood",
author = "Anders Etzerodt and Berg, {Ronan M G} and Plovsing, {Ronni R} and Andersen, {Morten N} and Magali Bebien and Mohamed Habbeddine and Toby Lawrence and M{\o}ller, {Holger J.} and Moestrup, {S{\o}ren K.}",
year = "2017",
doi = "10.1038/srep40286",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma

AU - Etzerodt, Anders

AU - Berg, Ronan M G

AU - Plovsing, Ronni R

AU - Andersen, Morten N

AU - Bebien, Magali

AU - Habbeddine, Mohamed

AU - Lawrence, Toby

AU - Møller, Holger J.

AU - Moestrup, Søren K.

PY - 2017

Y1 - 2017

N2 - CD163 is the macrophage receptor for uptake of hemoglobin-haptoglobin complexes. The human receptor can be shed from the macrophage surface owing to a cleavage site for the inflammation-inducible TACE/ADAM17 enzyme. Accordingly, plasma 'soluble CD163' (sCD163) has become a biomarker for macrophage activity and inflammation. The present study disclosed that 10% of sCD163 in healthy persons is actually extracellular vesicle (EV)-associated CD163 not being cleaved and shed. Endotoxin injection of human volunteers caused a selective increase in the ectodomain CD163, while septic patients exhibited high levels of both soluble ectodomain CD163 and extracellular vesicle (EV) CD163, the latter representing up 60% of total plasma CD163. A poor prognosis of septic patients measured as the sequential organ failure assessment (SOFA) score correlated with the increase in membrane-associated CD163. Our results show that soluble ectodomain CD163 and EV CD163 in plasma are part of separate macrophage response in the context of systemic inflammation. While that soluble ectodomain CD163 is released during the acute systemic inflammatory response, this is not the case for EV CD163 that instead may be released during a later phase of the inflammatory response. A separate measurement of the two forms of CD163 constituting 'soluble CD163' in plasma may therefore add to the diagnostic and prognostic value.

AB - CD163 is the macrophage receptor for uptake of hemoglobin-haptoglobin complexes. The human receptor can be shed from the macrophage surface owing to a cleavage site for the inflammation-inducible TACE/ADAM17 enzyme. Accordingly, plasma 'soluble CD163' (sCD163) has become a biomarker for macrophage activity and inflammation. The present study disclosed that 10% of sCD163 in healthy persons is actually extracellular vesicle (EV)-associated CD163 not being cleaved and shed. Endotoxin injection of human volunteers caused a selective increase in the ectodomain CD163, while septic patients exhibited high levels of both soluble ectodomain CD163 and extracellular vesicle (EV) CD163, the latter representing up 60% of total plasma CD163. A poor prognosis of septic patients measured as the sequential organ failure assessment (SOFA) score correlated with the increase in membrane-associated CD163. Our results show that soluble ectodomain CD163 and EV CD163 in plasma are part of separate macrophage response in the context of systemic inflammation. While that soluble ectodomain CD163 is released during the acute systemic inflammatory response, this is not the case for EV CD163 that instead may be released during a later phase of the inflammatory response. A separate measurement of the two forms of CD163 constituting 'soluble CD163' in plasma may therefore add to the diagnostic and prognostic value.

KW - Adult

KW - Aged

KW - Antigens, CD/blood

KW - Antigens, Differentiation, Myelomonocytic/blood

KW - Biomarkers/blood

KW - Endotoxins/administration & dosage

KW - Extracellular Vesicles/chemistry

KW - Female

KW - Haptoglobins/chemistry

KW - Healthy Volunteers

KW - Hemoglobins/chemistry

KW - Humans

KW - Inflammation/blood

KW - Macrophages/drug effects

KW - Male

KW - Middle Aged

KW - Prognosis

KW - Protein Isoforms/genetics

KW - Receptors, Cell Surface/blood

U2 - 10.1038/srep40286

DO - 10.1038/srep40286

M3 - Journal article

C2 - 28084321

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 40286

ER -

ID: 236992345