Skeletal muscle apolipoprotein B expression reduces muscular triglyceride accumulation

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Standard

Skeletal muscle apolipoprotein B expression reduces muscular triglyceride accumulation. / Bartels, Emil D; Ploug, Thorkil; Størling, Joachim; Mandrup-Poulsen, Thomas; Nielsen, Lars Bo.

I: Scandinavian Journal of Clinical & Laboratory Investigation, Bind 74, Nr. 4, 06.2014, s. 351-7.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Bartels, ED, Ploug, T, Størling, J, Mandrup-Poulsen, T & Nielsen, LB 2014, 'Skeletal muscle apolipoprotein B expression reduces muscular triglyceride accumulation', Scandinavian Journal of Clinical & Laboratory Investigation, bind 74, nr. 4, s. 351-7. https://doi.org/10.3109/00365513.2014.893446

APA

Bartels, E. D., Ploug, T., Størling, J., Mandrup-Poulsen, T., & Nielsen, L. B. (2014). Skeletal muscle apolipoprotein B expression reduces muscular triglyceride accumulation. Scandinavian Journal of Clinical & Laboratory Investigation, 74(4), 351-7. https://doi.org/10.3109/00365513.2014.893446

Vancouver

Bartels ED, Ploug T, Størling J, Mandrup-Poulsen T, Nielsen LB. Skeletal muscle apolipoprotein B expression reduces muscular triglyceride accumulation. Scandinavian Journal of Clinical & Laboratory Investigation. 2014 jun;74(4):351-7. https://doi.org/10.3109/00365513.2014.893446

Author

Bartels, Emil D ; Ploug, Thorkil ; Størling, Joachim ; Mandrup-Poulsen, Thomas ; Nielsen, Lars Bo. / Skeletal muscle apolipoprotein B expression reduces muscular triglyceride accumulation. I: Scandinavian Journal of Clinical & Laboratory Investigation. 2014 ; Bind 74, Nr. 4. s. 351-7.

Bibtex

@article{6450fc2a3b274239bbe5d667ba72d146,
title = "Skeletal muscle apolipoprotein B expression reduces muscular triglyceride accumulation",
abstract = "Abstract Background. Lipid accumulation in skeletal muscle is associated with impaired insulin sensitivity in type 2 diabetes. In cardiac myocytes, lipoprotein secretion controlled by apolipoproteinB (apoB) and microsomal triglyceride transfer protein (MTP) affects lipid homeostasis. Design. In this study, we investigated whether expression of a human apoB transgene affects triglyceride accumulation and insulin sensitivity in skeletal muscle in fat fed obese mice. Results. Expression of apoB and MTP mRNA and the human apoB transgene was seen in skeletal muscle of the transgene mice. Human apoB transgenic mice accumulated 28{\%} less triglycerides in skeletal myocytes after one year of fat-feeding as compared with WT mice (32 ± 5, n = 10 vs. 44 ± 4 nmol/mg ww, n = 13, p = 0.04). Moreover, expression of human apoB in fat-fed mice was associated with 32{\%} (p = 0.02) and 37{\%} (p = 0.01) lower plasma insulin levels after 9 and 12 months, respectively, improved intra peritoneal glucose tolerance after 6 months, and a trend towards increased insulin-stimulated glucose uptake in isolated skeletal muscle. Conclusions. The data suggests that overexpression of apoB decreases skeletal muscle lipid accumulation and attenuates peripheral insulin resistance in obese mice.",
author = "Bartels, {Emil D} and Thorkil Ploug and Joachim St{\o}rling and Thomas Mandrup-Poulsen and Nielsen, {Lars Bo}",
year = "2014",
month = "6",
doi = "10.3109/00365513.2014.893446",
language = "English",
volume = "74",
pages = "351--7",
journal = "Scandinavian Journal of Clinical & Laboratory Investigation",
issn = "0036-5513",
publisher = "Taylor & Francis",
number = "4",

}

RIS

TY - JOUR

T1 - Skeletal muscle apolipoprotein B expression reduces muscular triglyceride accumulation

AU - Bartels, Emil D

AU - Ploug, Thorkil

AU - Størling, Joachim

AU - Mandrup-Poulsen, Thomas

AU - Nielsen, Lars Bo

PY - 2014/6

Y1 - 2014/6

N2 - Abstract Background. Lipid accumulation in skeletal muscle is associated with impaired insulin sensitivity in type 2 diabetes. In cardiac myocytes, lipoprotein secretion controlled by apolipoproteinB (apoB) and microsomal triglyceride transfer protein (MTP) affects lipid homeostasis. Design. In this study, we investigated whether expression of a human apoB transgene affects triglyceride accumulation and insulin sensitivity in skeletal muscle in fat fed obese mice. Results. Expression of apoB and MTP mRNA and the human apoB transgene was seen in skeletal muscle of the transgene mice. Human apoB transgenic mice accumulated 28% less triglycerides in skeletal myocytes after one year of fat-feeding as compared with WT mice (32 ± 5, n = 10 vs. 44 ± 4 nmol/mg ww, n = 13, p = 0.04). Moreover, expression of human apoB in fat-fed mice was associated with 32% (p = 0.02) and 37% (p = 0.01) lower plasma insulin levels after 9 and 12 months, respectively, improved intra peritoneal glucose tolerance after 6 months, and a trend towards increased insulin-stimulated glucose uptake in isolated skeletal muscle. Conclusions. The data suggests that overexpression of apoB decreases skeletal muscle lipid accumulation and attenuates peripheral insulin resistance in obese mice.

AB - Abstract Background. Lipid accumulation in skeletal muscle is associated with impaired insulin sensitivity in type 2 diabetes. In cardiac myocytes, lipoprotein secretion controlled by apolipoproteinB (apoB) and microsomal triglyceride transfer protein (MTP) affects lipid homeostasis. Design. In this study, we investigated whether expression of a human apoB transgene affects triglyceride accumulation and insulin sensitivity in skeletal muscle in fat fed obese mice. Results. Expression of apoB and MTP mRNA and the human apoB transgene was seen in skeletal muscle of the transgene mice. Human apoB transgenic mice accumulated 28% less triglycerides in skeletal myocytes after one year of fat-feeding as compared with WT mice (32 ± 5, n = 10 vs. 44 ± 4 nmol/mg ww, n = 13, p = 0.04). Moreover, expression of human apoB in fat-fed mice was associated with 32% (p = 0.02) and 37% (p = 0.01) lower plasma insulin levels after 9 and 12 months, respectively, improved intra peritoneal glucose tolerance after 6 months, and a trend towards increased insulin-stimulated glucose uptake in isolated skeletal muscle. Conclusions. The data suggests that overexpression of apoB decreases skeletal muscle lipid accumulation and attenuates peripheral insulin resistance in obese mice.

U2 - 10.3109/00365513.2014.893446

DO - 10.3109/00365513.2014.893446

M3 - Journal article

C2 - 24673444

VL - 74

SP - 351

EP - 357

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

SN - 0036-5513

IS - 4

ER -

ID: 113810113