Simultaneous imaging of hyperpolarized [1,4-13 C2 ]fumarate, [1-13 C]pyruvate and 18 F-FDG in a rat model of necrosis in a clinical PET/MR scanner

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Simultaneous imaging of hyperpolarized [1,4-13 C2 ]fumarate, [1-13 C]pyruvate and 18 F-FDG in a rat model of necrosis in a clinical PET/MR scanner. / Eldirdiri, Abubakr; Clemmensen, Andreas; Bowen, Sean; Kjaer, Andreas; Ardenkjaer-Larsen, Jan Henrik.

I: N M R in Biomedicine, Bind 30, Nr. 12, e3803, 12.2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Eldirdiri, A, Clemmensen, A, Bowen, S, Kjaer, A & Ardenkjaer-Larsen, JH 2017, 'Simultaneous imaging of hyperpolarized [1,4-13 C2 ]fumarate, [1-13 C]pyruvate and 18 F-FDG in a rat model of necrosis in a clinical PET/MR scanner', N M R in Biomedicine, bind 30, nr. 12, e3803. https://doi.org/10.1002/nbm.3803

APA

Eldirdiri, A., Clemmensen, A., Bowen, S., Kjaer, A., & Ardenkjaer-Larsen, J. H. (2017). Simultaneous imaging of hyperpolarized [1,4-13 C2 ]fumarate, [1-13 C]pyruvate and 18 F-FDG in a rat model of necrosis in a clinical PET/MR scanner. N M R in Biomedicine, 30(12), [e3803]. https://doi.org/10.1002/nbm.3803

Vancouver

Eldirdiri A, Clemmensen A, Bowen S, Kjaer A, Ardenkjaer-Larsen JH. Simultaneous imaging of hyperpolarized [1,4-13 C2 ]fumarate, [1-13 C]pyruvate and 18 F-FDG in a rat model of necrosis in a clinical PET/MR scanner. N M R in Biomedicine. 2017 dec;30(12). e3803. https://doi.org/10.1002/nbm.3803

Author

Eldirdiri, Abubakr ; Clemmensen, Andreas ; Bowen, Sean ; Kjaer, Andreas ; Ardenkjaer-Larsen, Jan Henrik. / Simultaneous imaging of hyperpolarized [1,4-13 C2 ]fumarate, [1-13 C]pyruvate and 18 F-FDG in a rat model of necrosis in a clinical PET/MR scanner. I: N M R in Biomedicine. 2017 ; Bind 30, Nr. 12.

Bibtex

@article{b4fc6552176c4b23aa8a0dfdc49ab6ac,
title = "Simultaneous imaging of hyperpolarized [1,4-13 C2 ]fumarate, [1-13 C]pyruvate and 18 F-FDG in a rat model of necrosis in a clinical PET/MR scanner",
abstract = "A co-polarization scheme for [1,4-13 C2 ]fumarate and [1-13 C]pyruvate is presented to simultaneously assess necrosis and metabolism in rats with hyperpolarized 13 C magnetic resonance (MR). The co-polarization was performed in a SPINlab polarizer. In addition, the feasibility of simultaneous positron emission tomography (PET) and MR of small animals with a clinical PET/MR scanner is demonstrated. The hyperpolarized metabolic MR and PET was demonstrated in a rat model of necrosis. The polarization and T1 of the co-polarized [1,4-13 C2 ]fumarate and [1-13 C]pyruvate substrates were measured in vitro and compared with those obtained when the substrates were polarized individually. A polarization of 36 ± 4{\%} for fumarate and 37 ± 6{\%} for pyruvate was obtained. We found no significant difference in the polarization and T1 values between the dual and single substrate polarization. Rats weighing about 400 g were injected intramuscularly in one of the hind legs with 200 μL of turpentine to induce necrosis. Two hours later, 13 C metabolic maps were obtained with a chemical shift imaging sequence (16 × 16) with a resolution of 3.1 × 5.0 × 25.0 mm3 . The 13 C spectroscopic images were acquired in 12 s, followed by an 8-min 18 F-2-fluoro-2-deoxy-d-glucose (18 F-FDG) PET acquisition with a resolution of 3.5 mm. [1,4-13 C2 ]Malate was observed from the tissue injected with turpentine indicating necrosis. Normal [1-13 C]pyruvate metabolism and 18 F-FDG uptake were observed from the same tissue. The proposed co-polarization scheme provides a means to utilize multiple imaging agents simultaneously, and thus to probe various metabolic pathways in a single examination. Moreover, it demonstrates the feasibility of small animal research on a clinical PET/MR scanner for combined PET and hyperpolarized metabolic MR.",
author = "Abubakr Eldirdiri and Andreas Clemmensen and Sean Bowen and Andreas Kjaer and Ardenkjaer-Larsen, {Jan Henrik}",
note = "Copyright {\circledC} 2017 John Wiley & Sons, Ltd.",
year = "2017",
month = "12",
doi = "10.1002/nbm.3803",
language = "English",
volume = "30",
journal = "N M R in Biomedicine",
issn = "0952-3480",
publisher = "Wiley",
number = "12",

}

RIS

TY - JOUR

T1 - Simultaneous imaging of hyperpolarized [1,4-13 C2 ]fumarate, [1-13 C]pyruvate and 18 F-FDG in a rat model of necrosis in a clinical PET/MR scanner

AU - Eldirdiri, Abubakr

AU - Clemmensen, Andreas

AU - Bowen, Sean

AU - Kjaer, Andreas

AU - Ardenkjaer-Larsen, Jan Henrik

N1 - Copyright © 2017 John Wiley & Sons, Ltd.

PY - 2017/12

Y1 - 2017/12

N2 - A co-polarization scheme for [1,4-13 C2 ]fumarate and [1-13 C]pyruvate is presented to simultaneously assess necrosis and metabolism in rats with hyperpolarized 13 C magnetic resonance (MR). The co-polarization was performed in a SPINlab polarizer. In addition, the feasibility of simultaneous positron emission tomography (PET) and MR of small animals with a clinical PET/MR scanner is demonstrated. The hyperpolarized metabolic MR and PET was demonstrated in a rat model of necrosis. The polarization and T1 of the co-polarized [1,4-13 C2 ]fumarate and [1-13 C]pyruvate substrates were measured in vitro and compared with those obtained when the substrates were polarized individually. A polarization of 36 ± 4% for fumarate and 37 ± 6% for pyruvate was obtained. We found no significant difference in the polarization and T1 values between the dual and single substrate polarization. Rats weighing about 400 g were injected intramuscularly in one of the hind legs with 200 μL of turpentine to induce necrosis. Two hours later, 13 C metabolic maps were obtained with a chemical shift imaging sequence (16 × 16) with a resolution of 3.1 × 5.0 × 25.0 mm3 . The 13 C spectroscopic images were acquired in 12 s, followed by an 8-min 18 F-2-fluoro-2-deoxy-d-glucose (18 F-FDG) PET acquisition with a resolution of 3.5 mm. [1,4-13 C2 ]Malate was observed from the tissue injected with turpentine indicating necrosis. Normal [1-13 C]pyruvate metabolism and 18 F-FDG uptake were observed from the same tissue. The proposed co-polarization scheme provides a means to utilize multiple imaging agents simultaneously, and thus to probe various metabolic pathways in a single examination. Moreover, it demonstrates the feasibility of small animal research on a clinical PET/MR scanner for combined PET and hyperpolarized metabolic MR.

AB - A co-polarization scheme for [1,4-13 C2 ]fumarate and [1-13 C]pyruvate is presented to simultaneously assess necrosis and metabolism in rats with hyperpolarized 13 C magnetic resonance (MR). The co-polarization was performed in a SPINlab polarizer. In addition, the feasibility of simultaneous positron emission tomography (PET) and MR of small animals with a clinical PET/MR scanner is demonstrated. The hyperpolarized metabolic MR and PET was demonstrated in a rat model of necrosis. The polarization and T1 of the co-polarized [1,4-13 C2 ]fumarate and [1-13 C]pyruvate substrates were measured in vitro and compared with those obtained when the substrates were polarized individually. A polarization of 36 ± 4% for fumarate and 37 ± 6% for pyruvate was obtained. We found no significant difference in the polarization and T1 values between the dual and single substrate polarization. Rats weighing about 400 g were injected intramuscularly in one of the hind legs with 200 μL of turpentine to induce necrosis. Two hours later, 13 C metabolic maps were obtained with a chemical shift imaging sequence (16 × 16) with a resolution of 3.1 × 5.0 × 25.0 mm3 . The 13 C spectroscopic images were acquired in 12 s, followed by an 8-min 18 F-2-fluoro-2-deoxy-d-glucose (18 F-FDG) PET acquisition with a resolution of 3.5 mm. [1,4-13 C2 ]Malate was observed from the tissue injected with turpentine indicating necrosis. Normal [1-13 C]pyruvate metabolism and 18 F-FDG uptake were observed from the same tissue. The proposed co-polarization scheme provides a means to utilize multiple imaging agents simultaneously, and thus to probe various metabolic pathways in a single examination. Moreover, it demonstrates the feasibility of small animal research on a clinical PET/MR scanner for combined PET and hyperpolarized metabolic MR.

U2 - 10.1002/nbm.3803

DO - 10.1002/nbm.3803

M3 - Journal article

C2 - 29044751

VL - 30

JO - N M R in Biomedicine

JF - N M R in Biomedicine

SN - 0952-3480

IS - 12

M1 - e3803

ER -

ID: 189625002