Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. / Hotowy, Anna Malgorzata; Sawosz, Ewa; Pineda, Lane Manalili; Sawosz, Filip; Grodzik, Marta; Chwalibog, André.
I: Nanoscale Research Letters, Bind 7, Nr. 418, 2012.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart
AU - Hotowy, Anna Malgorzata
AU - Sawosz, Ewa
AU - Pineda, Lane Manalili
AU - Sawosz, Filip
AU - Grodzik, Marta
AU - Chwalibog, André
PY - 2012
Y1 - 2012
N2 - Nanoparticles of colloidal silver (AgNano) can influence gene expression. Concerning trials of AgNano application in poultry as antimicrobial and metabolic agents, it is useful to reveal whether they affect the expression of genes crucial for bird development. AgNano were administered to broiler chickens as a water solution in two concentrations (10 and 20 ppm). After dissection of the birds, breast muscles and hearts were collected. Gene expression of FGF2 and VEGF on the mRNA and protein levels were evaluated using qPCR and ELISA methods. The results for gene expression in breast muscle revealed significant changes on the mRNA level (FGF2 was up-regulated) but not on the protein level. In the heart, 20 ppm of AgNano in drinking water increased the expression of VEGFA, at the same time decreasing FGF2 expression both on the transcriptional and translational levels. Changes in the expression of these genes may lead to histological changes, but this needs to be proven using histological and immunohistochemical examination of tissues. In general, we showed that AgNano application in poultry feeding can influence the expression of FGF2 and VEGFA genes on the mRNA and protein levels in growing chicken.
AB - Nanoparticles of colloidal silver (AgNano) can influence gene expression. Concerning trials of AgNano application in poultry as antimicrobial and metabolic agents, it is useful to reveal whether they affect the expression of genes crucial for bird development. AgNano were administered to broiler chickens as a water solution in two concentrations (10 and 20 ppm). After dissection of the birds, breast muscles and hearts were collected. Gene expression of FGF2 and VEGF on the mRNA and protein levels were evaluated using qPCR and ELISA methods. The results for gene expression in breast muscle revealed significant changes on the mRNA level (FGF2 was up-regulated) but not on the protein level. In the heart, 20 ppm of AgNano in drinking water increased the expression of VEGFA, at the same time decreasing FGF2 expression both on the transcriptional and translational levels. Changes in the expression of these genes may lead to histological changes, but this needs to be proven using histological and immunohistochemical examination of tissues. In general, we showed that AgNano application in poultry feeding can influence the expression of FGF2 and VEGFA genes on the mRNA and protein levels in growing chicken.
KW - Former LIFE faculty
KW - Silver nanoparticles
KW - VEGFA
KW - FGF2
KW - Muscle
KW - Heart
KW - Chicken
U2 - 10.1186/1556-276X-7-418
DO - 10.1186/1556-276X-7-418
M3 - Journal article
VL - 7
JO - Nanoscale Research Letters
JF - Nanoscale Research Letters
SN - 1931-7573
IS - 418
ER -
ID: 40915182