Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors

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Standard

Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors. / Møldrup, Annette; Billestrup, N; Nielsen, Jens Høiriis.

I: The Journal of Biological Chemistry, Bind 265, Nr. 15, 25.05.1990, s. 8686-90.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Møldrup, A, Billestrup, N & Nielsen, JH 1990, 'Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors', The Journal of Biological Chemistry, bind 265, nr. 15, s. 8686-90.

APA

Møldrup, A., Billestrup, N., & Nielsen, J. H. (1990). Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors. The Journal of Biological Chemistry, 265(15), 8686-90.

Vancouver

Møldrup A, Billestrup N, Nielsen JH. Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors. The Journal of Biological Chemistry. 1990 maj 25;265(15):8686-90.

Author

Møldrup, Annette ; Billestrup, N ; Nielsen, Jens Høiriis. / Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors. I: The Journal of Biological Chemistry. 1990 ; Bind 265, Nr. 15. s. 8686-90.

Bibtex

@article{7e8b5c57ea284b8c81374da81e60c57c,
title = "Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors",
abstract = "Insulin-producing rat islet RIN-5AH tumor cells express multiple binding sites for human growth hormone (hGH). The effect of rat growth hormone (rGH), rat prolactin (rPRL), and human placental lactogen (hPL) on the binding of 125I-labeled hGH (125I-hGH) to RIN-5AH cells revealed the presence of both lactogen and somatogen receptor populations. Covalent cross-linking of 125I-hGH, 125I-rGH, and 125I-rPRL to the RIN cells identified a 115-kDa somatogen receptor protein that binds hGH and rGH but not rPRL and hPL, and a 95-kDa lactogen receptor protein that binds hGH, rPRL, and hPL but not rGH. Antiserum directed against the 37.5- and 40.7-kDa GH-binding proteins of mouse hepatic tissue specifically recognized the 115-kDa protein cross-linked with 125I-hGH, whereas a monoclonal antibody raised against the hepatic 42-kDa rPRL receptor recognized the 95-kDa protein cross-linked with 125I-hGH. Northern blot analysis of RNA from RIN-5AH cells using an rGH receptor-specific cDNA probe showed three transcripts of 5.8, 1.8, and 1.5 kilobases, respectively. Analysis with an rPRL receptor-specific cDNA probe revealed the presence of a predominant 3.5-kilobase transcript. We conclude that the insulin-producing cells express large forms of both the GH and PRL receptors which are structurally related to those of hepatic tissue.",
keywords = "Adenoma, Islet Cell, Animals, Blotting, Northern, Cell Line, Cross-Linking Reagents, Growth Hormone, Insulinoma, Kinetics, Liver, Molecular Weight, Pancreatic Neoplasms, RNA, RNA, Neoplasm, Rats, Receptors, Prolactin, Receptors, Somatotropin",
author = "Annette M{\o}ldrup and N Billestrup and Nielsen, {Jens H{\o}iriis}",
year = "1990",
month = may,
day = "25",
language = "English",
volume = "265",
pages = "8686--90",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "15",

}

RIS

TY - JOUR

T1 - Rat insulinoma cells express both a 115-kDa growth hormone receptor and a 95-kDa prolactin receptor structurally related to the hepatic receptors

AU - Møldrup, Annette

AU - Billestrup, N

AU - Nielsen, Jens Høiriis

PY - 1990/5/25

Y1 - 1990/5/25

N2 - Insulin-producing rat islet RIN-5AH tumor cells express multiple binding sites for human growth hormone (hGH). The effect of rat growth hormone (rGH), rat prolactin (rPRL), and human placental lactogen (hPL) on the binding of 125I-labeled hGH (125I-hGH) to RIN-5AH cells revealed the presence of both lactogen and somatogen receptor populations. Covalent cross-linking of 125I-hGH, 125I-rGH, and 125I-rPRL to the RIN cells identified a 115-kDa somatogen receptor protein that binds hGH and rGH but not rPRL and hPL, and a 95-kDa lactogen receptor protein that binds hGH, rPRL, and hPL but not rGH. Antiserum directed against the 37.5- and 40.7-kDa GH-binding proteins of mouse hepatic tissue specifically recognized the 115-kDa protein cross-linked with 125I-hGH, whereas a monoclonal antibody raised against the hepatic 42-kDa rPRL receptor recognized the 95-kDa protein cross-linked with 125I-hGH. Northern blot analysis of RNA from RIN-5AH cells using an rGH receptor-specific cDNA probe showed three transcripts of 5.8, 1.8, and 1.5 kilobases, respectively. Analysis with an rPRL receptor-specific cDNA probe revealed the presence of a predominant 3.5-kilobase transcript. We conclude that the insulin-producing cells express large forms of both the GH and PRL receptors which are structurally related to those of hepatic tissue.

AB - Insulin-producing rat islet RIN-5AH tumor cells express multiple binding sites for human growth hormone (hGH). The effect of rat growth hormone (rGH), rat prolactin (rPRL), and human placental lactogen (hPL) on the binding of 125I-labeled hGH (125I-hGH) to RIN-5AH cells revealed the presence of both lactogen and somatogen receptor populations. Covalent cross-linking of 125I-hGH, 125I-rGH, and 125I-rPRL to the RIN cells identified a 115-kDa somatogen receptor protein that binds hGH and rGH but not rPRL and hPL, and a 95-kDa lactogen receptor protein that binds hGH, rPRL, and hPL but not rGH. Antiserum directed against the 37.5- and 40.7-kDa GH-binding proteins of mouse hepatic tissue specifically recognized the 115-kDa protein cross-linked with 125I-hGH, whereas a monoclonal antibody raised against the hepatic 42-kDa rPRL receptor recognized the 95-kDa protein cross-linked with 125I-hGH. Northern blot analysis of RNA from RIN-5AH cells using an rGH receptor-specific cDNA probe showed three transcripts of 5.8, 1.8, and 1.5 kilobases, respectively. Analysis with an rPRL receptor-specific cDNA probe revealed the presence of a predominant 3.5-kilobase transcript. We conclude that the insulin-producing cells express large forms of both the GH and PRL receptors which are structurally related to those of hepatic tissue.

KW - Adenoma, Islet Cell

KW - Animals

KW - Blotting, Northern

KW - Cell Line

KW - Cross-Linking Reagents

KW - Growth Hormone

KW - Insulinoma

KW - Kinetics

KW - Liver

KW - Molecular Weight

KW - Pancreatic Neoplasms

KW - RNA

KW - RNA, Neoplasm

KW - Rats

KW - Receptors, Prolactin

KW - Receptors, Somatotropin

M3 - Journal article

C2 - 1692834

VL - 265

SP - 8686

EP - 8690

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 15

ER -

ID: 47974053