Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice

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Standard

Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice. / Sylow, Lykke; Laurent, Ida; Kleinert, Maximilian; Møller, Lisbeth Liliendal Valbjørn; Ploug, Thorkil; Schjerling, Peter; Bilan, Philip J.; Klip, Amira; Jensen, Thomas Elbenhardt; Richter, Erik A.

I: Journal of Physiology, Bind 594, Nr. 17, 2016, s. 4997-5008.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Sylow, L, Laurent, I, Kleinert, M, Møller, LLV, Ploug, T, Schjerling, P, Bilan, PJ, Klip, A, Jensen, TE & Richter, EA 2016, 'Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice', Journal of Physiology, bind 594, nr. 17, s. 4997-5008. https://doi.org/10.1113/JP272039

APA

Sylow, L., Laurent, I., Kleinert, M., Møller, L. L. V., Ploug, T., Schjerling, P., ... Richter, E. A. (2016). Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice. Journal of Physiology, 594(17), 4997-5008. https://doi.org/10.1113/JP272039

Vancouver

Sylow L, Laurent I, Kleinert M, Møller LLV, Ploug T, Schjerling P o.a. Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice. Journal of Physiology. 2016;594(17):4997-5008. https://doi.org/10.1113/JP272039

Author

Sylow, Lykke ; Laurent, Ida ; Kleinert, Maximilian ; Møller, Lisbeth Liliendal Valbjørn ; Ploug, Thorkil ; Schjerling, Peter ; Bilan, Philip J. ; Klip, Amira ; Jensen, Thomas Elbenhardt ; Richter, Erik A. / Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice. I: Journal of Physiology. 2016 ; Bind 594, Nr. 17. s. 4997-5008.

Bibtex

@article{edfa294e522c4c3784296c66d78bfbca,
title = "Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice",
abstract = "Exercise increase skeletal muscle energy turnover and one of the important substrates for the working muscle is glucose taken up from the blood. Despite extensive efforts, the signaling mechanisms vital for glucose uptake during exercise are not yet fully understood but the GTPase Rac1 is a candidate molecule. This study investigated the role of Rac1 in muscle glucose uptake and substrate utilization during treadmill exercise in mice in vivo. Exercise-induced uptake of radiolabelled 2-deoxyglucose (2-DG) at 65{\%} max running capacity was blocked in soleus and decreased by 80 and 60{\%} in gastrocnemius and tibialis anterior muscles, respectively, in muscle-specific inducible Rac1 knockout (mKO) mice compared to wildtype littermates. By developing an assay to quantify endogenous GLUT4 translocation, we observed that GLUT4 content at the sarcolemma in response to exercise was reduced in Rac1 mKO muscle. Our findings implicate Rac1 as a regulatory element critical for controlling glucose uptake during exercise via regulation of GLUT4 translocation. This article is protected by copyright. All rights reserved.",
author = "Lykke Sylow and Ida Laurent and Maximilian Kleinert and M{\o}ller, {Lisbeth Liliendal Valbj{\o}rn} and Thorkil Ploug and Peter Schjerling and Bilan, {Philip J.} and Amira Klip and Jensen, {Thomas Elbenhardt} and Richter, {Erik A.}",
note = "CURIS 2016 NEXS 186",
year = "2016",
doi = "10.1113/JP272039",
language = "English",
volume = "594",
pages = "4997--5008",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "17",

}

RIS

TY - JOUR

T1 - Rac1 governs exercise-stimulated glucose uptake in skeletal muscle through regulation of GLUT4 translocation in mice

AU - Sylow, Lykke

AU - Laurent, Ida

AU - Kleinert, Maximilian

AU - Møller, Lisbeth Liliendal Valbjørn

AU - Ploug, Thorkil

AU - Schjerling, Peter

AU - Bilan, Philip J.

AU - Klip, Amira

AU - Jensen, Thomas Elbenhardt

AU - Richter, Erik A.

N1 - CURIS 2016 NEXS 186

PY - 2016

Y1 - 2016

N2 - Exercise increase skeletal muscle energy turnover and one of the important substrates for the working muscle is glucose taken up from the blood. Despite extensive efforts, the signaling mechanisms vital for glucose uptake during exercise are not yet fully understood but the GTPase Rac1 is a candidate molecule. This study investigated the role of Rac1 in muscle glucose uptake and substrate utilization during treadmill exercise in mice in vivo. Exercise-induced uptake of radiolabelled 2-deoxyglucose (2-DG) at 65% max running capacity was blocked in soleus and decreased by 80 and 60% in gastrocnemius and tibialis anterior muscles, respectively, in muscle-specific inducible Rac1 knockout (mKO) mice compared to wildtype littermates. By developing an assay to quantify endogenous GLUT4 translocation, we observed that GLUT4 content at the sarcolemma in response to exercise was reduced in Rac1 mKO muscle. Our findings implicate Rac1 as a regulatory element critical for controlling glucose uptake during exercise via regulation of GLUT4 translocation. This article is protected by copyright. All rights reserved.

AB - Exercise increase skeletal muscle energy turnover and one of the important substrates for the working muscle is glucose taken up from the blood. Despite extensive efforts, the signaling mechanisms vital for glucose uptake during exercise are not yet fully understood but the GTPase Rac1 is a candidate molecule. This study investigated the role of Rac1 in muscle glucose uptake and substrate utilization during treadmill exercise in mice in vivo. Exercise-induced uptake of radiolabelled 2-deoxyglucose (2-DG) at 65% max running capacity was blocked in soleus and decreased by 80 and 60% in gastrocnemius and tibialis anterior muscles, respectively, in muscle-specific inducible Rac1 knockout (mKO) mice compared to wildtype littermates. By developing an assay to quantify endogenous GLUT4 translocation, we observed that GLUT4 content at the sarcolemma in response to exercise was reduced in Rac1 mKO muscle. Our findings implicate Rac1 as a regulatory element critical for controlling glucose uptake during exercise via regulation of GLUT4 translocation. This article is protected by copyright. All rights reserved.

U2 - 10.1113/JP272039

DO - 10.1113/JP272039

M3 - Journal article

C2 - 27061726

VL - 594

SP - 4997

EP - 5008

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 17

ER -

ID: 161001827