Quantification of low molecular weight selenium metabolites in human plasma after treatment with selenite in pharmacological doses by LC-ICP-MS

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Standard

Quantification of low molecular weight selenium metabolites in human plasma after treatment with selenite in pharmacological doses by LC-ICP-MS. / Flouda, Konstantina; Dersch, Julie Maria; Gabel-Jensen, Charlotte; Stürup, Stefan; Mistra, Sougat; Björnstedt, Mikael; Gammelgaard, Bente.

I: Analytical and Bioanalytical Chemistry, Bind 408, Nr. 9, 2016, s. 2293-2301.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Flouda, K, Dersch, JM, Gabel-Jensen, C, Stürup, S, Mistra, S, Björnstedt, M & Gammelgaard, B 2016, 'Quantification of low molecular weight selenium metabolites in human plasma after treatment with selenite in pharmacological doses by LC-ICP-MS', Analytical and Bioanalytical Chemistry, bind 408, nr. 9, s. 2293-2301. https://doi.org/10.1007/s00216-016-9325-2

APA

Flouda, K., Dersch, J. M., Gabel-Jensen, C., Stürup, S., Mistra, S., Björnstedt, M., & Gammelgaard, B. (2016). Quantification of low molecular weight selenium metabolites in human plasma after treatment with selenite in pharmacological doses by LC-ICP-MS. Analytical and Bioanalytical Chemistry, 408(9), 2293-2301. https://doi.org/10.1007/s00216-016-9325-2

Vancouver

Flouda K, Dersch JM, Gabel-Jensen C, Stürup S, Mistra S, Björnstedt M o.a. Quantification of low molecular weight selenium metabolites in human plasma after treatment with selenite in pharmacological doses by LC-ICP-MS. Analytical and Bioanalytical Chemistry. 2016;408(9):2293-2301. https://doi.org/10.1007/s00216-016-9325-2

Author

Flouda, Konstantina ; Dersch, Julie Maria ; Gabel-Jensen, Charlotte ; Stürup, Stefan ; Mistra, Sougat ; Björnstedt, Mikael ; Gammelgaard, Bente. / Quantification of low molecular weight selenium metabolites in human plasma after treatment with selenite in pharmacological doses by LC-ICP-MS. I: Analytical and Bioanalytical Chemistry. 2016 ; Bind 408, Nr. 9. s. 2293-2301.

Bibtex

@article{5dabfbe4a6824c3aafc7a3dbfbddd086,
title = "Quantification of low molecular weight selenium metabolites in human plasma after treatment with selenite in pharmacological doses by LC-ICP-MS",
abstract = "The paper presents an analytical method for quantification of low molecular weight (LMW) selenium compounds in human plasma based on liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS) and post column isotope dilution-based quantification. Prior to analysis, samples were ultrafiltrated using a cut-off value of 3000 Da. The method was validated in aqueous solution as well as plasma using standards of selenomethionine (SeMet), Se-methylselenocysteine (MeSeCys), selenite, and the selenosugar Se-methylseleno-N-acetylgalactosamine (SeGal) for linearity, precision, recoveries, and limits of detection and quantitation with satisfactory results. The method was applied for analysis of a set of plasma samples from cancer patients receiving selenite treatment in a clinical trial. Three LMW selenium compounds were observed. The main compounds, SeGal and selenite were tentatively identified by retention time matching with standards in different chromatographic systems, while the third minor compound was not identified. The identity of the selenosugar was verified by ESI-MS-MS product ion scanning, while selenite was identified indirectly as the glutathione (GSH) reaction product, GS-Se-SG.",
author = "Konstantina Flouda and Dersch, {Julie Maria} and Charlotte Gabel-Jensen and Stefan St{\"u}rup and Sougat Mistra and Mikael Bj{\"o}rnstedt and Bente Gammelgaard",
year = "2016",
doi = "10.1007/s00216-016-9325-2",
language = "English",
volume = "408",
pages = "2293--2301",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer",
number = "9",

}

RIS

TY - JOUR

T1 - Quantification of low molecular weight selenium metabolites in human plasma after treatment with selenite in pharmacological doses by LC-ICP-MS

AU - Flouda, Konstantina

AU - Dersch, Julie Maria

AU - Gabel-Jensen, Charlotte

AU - Stürup, Stefan

AU - Mistra, Sougat

AU - Björnstedt, Mikael

AU - Gammelgaard, Bente

PY - 2016

Y1 - 2016

N2 - The paper presents an analytical method for quantification of low molecular weight (LMW) selenium compounds in human plasma based on liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS) and post column isotope dilution-based quantification. Prior to analysis, samples were ultrafiltrated using a cut-off value of 3000 Da. The method was validated in aqueous solution as well as plasma using standards of selenomethionine (SeMet), Se-methylselenocysteine (MeSeCys), selenite, and the selenosugar Se-methylseleno-N-acetylgalactosamine (SeGal) for linearity, precision, recoveries, and limits of detection and quantitation with satisfactory results. The method was applied for analysis of a set of plasma samples from cancer patients receiving selenite treatment in a clinical trial. Three LMW selenium compounds were observed. The main compounds, SeGal and selenite were tentatively identified by retention time matching with standards in different chromatographic systems, while the third minor compound was not identified. The identity of the selenosugar was verified by ESI-MS-MS product ion scanning, while selenite was identified indirectly as the glutathione (GSH) reaction product, GS-Se-SG.

AB - The paper presents an analytical method for quantification of low molecular weight (LMW) selenium compounds in human plasma based on liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS) and post column isotope dilution-based quantification. Prior to analysis, samples were ultrafiltrated using a cut-off value of 3000 Da. The method was validated in aqueous solution as well as plasma using standards of selenomethionine (SeMet), Se-methylselenocysteine (MeSeCys), selenite, and the selenosugar Se-methylseleno-N-acetylgalactosamine (SeGal) for linearity, precision, recoveries, and limits of detection and quantitation with satisfactory results. The method was applied for analysis of a set of plasma samples from cancer patients receiving selenite treatment in a clinical trial. Three LMW selenium compounds were observed. The main compounds, SeGal and selenite were tentatively identified by retention time matching with standards in different chromatographic systems, while the third minor compound was not identified. The identity of the selenosugar was verified by ESI-MS-MS product ion scanning, while selenite was identified indirectly as the glutathione (GSH) reaction product, GS-Se-SG.

U2 - 10.1007/s00216-016-9325-2

DO - 10.1007/s00216-016-9325-2

M3 - Journal article

C2 - 26832729

VL - 408

SP - 2293

EP - 2301

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 9

ER -

ID: 161446670