Pre-embedding staining of single muscle fibers for light and electron microscopy studies of subcellular organization

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Standard

Pre-embedding staining of single muscle fibers for light and electron microscopy studies of subcellular organization. / Ralston, E; Ploug, Thorkil.

I: Scanning Microscopy Supplement, Bind 10, 1996, s. 249-59; discussion 259-60.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ralston, E & Ploug, T 1996, 'Pre-embedding staining of single muscle fibers for light and electron microscopy studies of subcellular organization', Scanning Microscopy Supplement, bind 10, s. 249-59; discussion 259-60.

APA

Ralston, E., & Ploug, T. (1996). Pre-embedding staining of single muscle fibers for light and electron microscopy studies of subcellular organization. Scanning Microscopy Supplement, 10, 249-59; discussion 259-60.

Vancouver

Ralston E, Ploug T. Pre-embedding staining of single muscle fibers for light and electron microscopy studies of subcellular organization. Scanning Microscopy Supplement. 1996;10:249-59; discussion 259-60.

Author

Ralston, E ; Ploug, Thorkil. / Pre-embedding staining of single muscle fibers for light and electron microscopy studies of subcellular organization. I: Scanning Microscopy Supplement. 1996 ; Bind 10. s. 249-59; discussion 259-60.

Bibtex

@article{2e40de7aa69f4d4099f1589662e58d75,
title = "Pre-embedding staining of single muscle fibers for light and electron microscopy studies of subcellular organization",
abstract = "Skeletal muscle fibers are large, multinucleated cells which pose a challenge to the morphologist. In the course of studies of the distribution of the glucose transporter GLUT4, in muscle, we have compared different preparative procedures, for both light (LM) and electron microscopy (EM) immunocytochemistry. Here we show that pre-embedding staining of single teased fibers, or of single enzymatically dissociated fibers, has several advantages over the use of sections for observing discrete patterns that extend over long distances in the cells. We report on an optimization study carried out to establish fixation and permeabilization conditions for EM immunogold labeling of the fibers. We find that a simple fixation with depolymerized paraformaldehyde alone, followed by permeabilization with 0.01% saponin, offers the best compromise between the conflicting demands of unhindered tissue penetration and morphology preservation.",
keywords = "Animals, Cell Nucleus, Formaldehyde, Glucose, Glucose Transporter Type 4, Histocytological Preparation Techniques, Immunohistochemistry, Male, Microscopy, Confocal, Microscopy, Fluorescence, Microscopy, Immunoelectron, Monosaccharide Transport Proteins, Muscle Fibers, Skeletal, Muscle Proteins, Muscle, Skeletal, Neuromuscular Junction, Rats, Rats, Wistar, Staining and Labeling",
author = "E Ralston and Thorkil Ploug",
year = "1996",
language = "English",
volume = "10",
pages = "249--59; discussion 259--60",
journal = "Scanning microscopy. Supplement",
issn = "0892-953X",
publisher = "Scanning Microscopy International",

}

RIS

TY - JOUR

T1 - Pre-embedding staining of single muscle fibers for light and electron microscopy studies of subcellular organization

AU - Ralston, E

AU - Ploug, Thorkil

PY - 1996

Y1 - 1996

N2 - Skeletal muscle fibers are large, multinucleated cells which pose a challenge to the morphologist. In the course of studies of the distribution of the glucose transporter GLUT4, in muscle, we have compared different preparative procedures, for both light (LM) and electron microscopy (EM) immunocytochemistry. Here we show that pre-embedding staining of single teased fibers, or of single enzymatically dissociated fibers, has several advantages over the use of sections for observing discrete patterns that extend over long distances in the cells. We report on an optimization study carried out to establish fixation and permeabilization conditions for EM immunogold labeling of the fibers. We find that a simple fixation with depolymerized paraformaldehyde alone, followed by permeabilization with 0.01% saponin, offers the best compromise between the conflicting demands of unhindered tissue penetration and morphology preservation.

AB - Skeletal muscle fibers are large, multinucleated cells which pose a challenge to the morphologist. In the course of studies of the distribution of the glucose transporter GLUT4, in muscle, we have compared different preparative procedures, for both light (LM) and electron microscopy (EM) immunocytochemistry. Here we show that pre-embedding staining of single teased fibers, or of single enzymatically dissociated fibers, has several advantages over the use of sections for observing discrete patterns that extend over long distances in the cells. We report on an optimization study carried out to establish fixation and permeabilization conditions for EM immunogold labeling of the fibers. We find that a simple fixation with depolymerized paraformaldehyde alone, followed by permeabilization with 0.01% saponin, offers the best compromise between the conflicting demands of unhindered tissue penetration and morphology preservation.

KW - Animals

KW - Cell Nucleus

KW - Formaldehyde

KW - Glucose

KW - Glucose Transporter Type 4

KW - Histocytological Preparation Techniques

KW - Immunohistochemistry

KW - Male

KW - Microscopy, Confocal

KW - Microscopy, Fluorescence

KW - Microscopy, Immunoelectron

KW - Monosaccharide Transport Proteins

KW - Muscle Fibers, Skeletal

KW - Muscle Proteins

KW - Muscle, Skeletal

KW - Neuromuscular Junction

KW - Rats

KW - Rats, Wistar

KW - Staining and Labeling

M3 - Journal article

C2 - 9601544

VL - 10

SP - 249-59; discussion 259-60

JO - Scanning microscopy. Supplement

JF - Scanning microscopy. Supplement

SN - 0892-953X

ER -

ID: 123665940