Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Alexey Brazhe
  • Nadezda Brazhe
  • Georgy V Maksimov
  • Pavel S Ignatyev
  • Andrey B Rubin
  • Erik Mosekilde
  • Sosnovtseva, Olga
We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.
OriginalsprogEngelsk
TidsskriftJournal of Biomedical Optics
Vol/bind13
Udgave nummer3
Sider (fra-til)034004
ISSN1083-3668
DOI
StatusUdgivet - 2008

ID: 33812429