Pharmacological activation of TGR5 promotes intestinal growth via a GLP-2 dependent pathway in mice

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Pharmacological activation of TGR5 promotes intestinal growth via a GLP-2 dependent pathway in mice. / Hunt, Jenna Elizabeth; Billeschou, Anna; Windeløv, Johanne Agerlin; Hartmann, Bolette; Ullmer, Christoph; Holst, Jens J; Kissow, Hannelouise.

I: American Journal of Physiology: Gastrointestinal and Liver Physiology, Bind 318, Nr. 6, 2020, s. G980-G987.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Hunt, JE, Billeschou, A, Windeløv, JA, Hartmann, B, Ullmer, C, Holst, JJ & Kissow, H 2020, 'Pharmacological activation of TGR5 promotes intestinal growth via a GLP-2 dependent pathway in mice', American Journal of Physiology: Gastrointestinal and Liver Physiology, bind 318, nr. 6, s. G980-G987. https://doi.org/10.1152/ajpgi.00062.2020

APA

Hunt, J. E., Billeschou, A., Windeløv, J. A., Hartmann, B., Ullmer, C., Holst, J. J., & Kissow, H. (2020). Pharmacological activation of TGR5 promotes intestinal growth via a GLP-2 dependent pathway in mice. American Journal of Physiology: Gastrointestinal and Liver Physiology, 318(6), G980-G987. https://doi.org/10.1152/ajpgi.00062.2020

Vancouver

Hunt JE, Billeschou A, Windeløv JA, Hartmann B, Ullmer C, Holst JJ o.a. Pharmacological activation of TGR5 promotes intestinal growth via a GLP-2 dependent pathway in mice. American Journal of Physiology: Gastrointestinal and Liver Physiology. 2020;318(6):G980-G987. https://doi.org/10.1152/ajpgi.00062.2020

Author

Hunt, Jenna Elizabeth ; Billeschou, Anna ; Windeløv, Johanne Agerlin ; Hartmann, Bolette ; Ullmer, Christoph ; Holst, Jens J ; Kissow, Hannelouise. / Pharmacological activation of TGR5 promotes intestinal growth via a GLP-2 dependent pathway in mice. I: American Journal of Physiology: Gastrointestinal and Liver Physiology. 2020 ; Bind 318, Nr. 6. s. G980-G987.

Bibtex

@article{f3189bee97754d9489dc1897e8a639bd,
title = "Pharmacological activation of TGR5 promotes intestinal growth via a GLP-2 dependent pathway in mice",
abstract = "Glucagon-like peptide-1 and 2 (GLP-1 and GLP-2) secreting L cells have been shown to express the bile acid receptor takeda-G-protein-receptor-5 (TGR5) and increase secretion upon receptor activation. Previous studies have explored GLP-1 secretion following acute TGR5 activation but chronic activation and GLP-2 responses have not been characterized. In this study, we aimed to investigate the consequences of pharmacological TGR5 receptor activation on L cell hormone production in vivo using the specific TGR5 agonist RO5527239 and the GLP-2 receptor knockout mouse. Here, we show 1) TGR5 receptor activation led to increased GLP-1 and GLP-2 content in the colon, which 2) was associated with an increased small intestinal weight that 3) was GLP-2 dependent. Additionally, we report that TGR5 mediated gallbladder filling occurred independently of GLP-2 signaling. In conclusion, we demonstrate that pharmacological TGR5 receptor activation stimulates L cells triggering GLP-2 dependent intestinal adaption in mice.",
author = "Hunt, {Jenna Elizabeth} and Anna Billeschou and Windel{\o}v, {Johanne Agerlin} and Bolette Hartmann and Christoph Ullmer and Holst, {Jens J} and Hannelouise Kissow",
year = "2020",
doi = "10.1152/ajpgi.00062.2020",
language = "English",
volume = "318",
pages = "G980--G987",
journal = "American Journal of Physiology: Gastrointestinal and Liver Physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "6",

}

RIS

TY - JOUR

T1 - Pharmacological activation of TGR5 promotes intestinal growth via a GLP-2 dependent pathway in mice

AU - Hunt, Jenna Elizabeth

AU - Billeschou, Anna

AU - Windeløv, Johanne Agerlin

AU - Hartmann, Bolette

AU - Ullmer, Christoph

AU - Holst, Jens J

AU - Kissow, Hannelouise

PY - 2020

Y1 - 2020

N2 - Glucagon-like peptide-1 and 2 (GLP-1 and GLP-2) secreting L cells have been shown to express the bile acid receptor takeda-G-protein-receptor-5 (TGR5) and increase secretion upon receptor activation. Previous studies have explored GLP-1 secretion following acute TGR5 activation but chronic activation and GLP-2 responses have not been characterized. In this study, we aimed to investigate the consequences of pharmacological TGR5 receptor activation on L cell hormone production in vivo using the specific TGR5 agonist RO5527239 and the GLP-2 receptor knockout mouse. Here, we show 1) TGR5 receptor activation led to increased GLP-1 and GLP-2 content in the colon, which 2) was associated with an increased small intestinal weight that 3) was GLP-2 dependent. Additionally, we report that TGR5 mediated gallbladder filling occurred independently of GLP-2 signaling. In conclusion, we demonstrate that pharmacological TGR5 receptor activation stimulates L cells triggering GLP-2 dependent intestinal adaption in mice.

AB - Glucagon-like peptide-1 and 2 (GLP-1 and GLP-2) secreting L cells have been shown to express the bile acid receptor takeda-G-protein-receptor-5 (TGR5) and increase secretion upon receptor activation. Previous studies have explored GLP-1 secretion following acute TGR5 activation but chronic activation and GLP-2 responses have not been characterized. In this study, we aimed to investigate the consequences of pharmacological TGR5 receptor activation on L cell hormone production in vivo using the specific TGR5 agonist RO5527239 and the GLP-2 receptor knockout mouse. Here, we show 1) TGR5 receptor activation led to increased GLP-1 and GLP-2 content in the colon, which 2) was associated with an increased small intestinal weight that 3) was GLP-2 dependent. Additionally, we report that TGR5 mediated gallbladder filling occurred independently of GLP-2 signaling. In conclusion, we demonstrate that pharmacological TGR5 receptor activation stimulates L cells triggering GLP-2 dependent intestinal adaption in mice.

U2 - 10.1152/ajpgi.00062.2020

DO - 10.1152/ajpgi.00062.2020

M3 - Journal article

C2 - 32308039

VL - 318

SP - G980-G987

JO - American Journal of Physiology: Gastrointestinal and Liver Physiology

JF - American Journal of Physiology: Gastrointestinal and Liver Physiology

SN - 0193-1857

IS - 6

ER -

ID: 239914310