Overproduction of PIB-Type ATPases
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.
Originalsprog | Engelsk |
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Titel | P-Type ATPases : Methods and Protocols |
Redaktører | Maike Bublitz |
Antal sider | 8 |
Vol/bind | 1377 |
Forlag | Springer |
Publikationsdato | 2016 |
Sider | 29-36 |
Kapitel | 5 |
ISBN (Trykt) | 978-1-4939-3178-1 |
ISBN (Elektronisk) | 978-1-4939-3179-8 |
DOI | |
Status | Udgivet - 2016 |
Navn | Methods in Molecular Biology |
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Vol/bind | 1377 |
ISSN | 1064-3745 |
ID: 171590537