Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector

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Neodymium-140 DOTA-LM3 : Evaluation of anGenerator for PET with a Non-Internalizing Vector. / Severin, Gregory W; Kristensen, Lotte K; Nielsen, Carsten H; Fonslet, Jesper; Jensen, Andreas I; Frellsen, Anders F; Jensen, K M; Elema, Dennis R; Maecke, Helmut; Kjær, Andreas; Johnston, Karl; Köster, Ulli.

I: Frontiers in Medicine, Bind 4, 98, 2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Severin, GW, Kristensen, LK, Nielsen, CH, Fonslet, J, Jensen, AI, Frellsen, AF, Jensen, KM, Elema, DR, Maecke, H, Kjær, A, Johnston, K & Köster, U 2017, 'Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector', Frontiers in Medicine, bind 4, 98. https://doi.org/10.3389/fmed.2017.00098

APA

Severin, G. W., Kristensen, L. K., Nielsen, C. H., Fonslet, J., Jensen, A. I., Frellsen, A. F., Jensen, K. M., Elema, D. R., Maecke, H., Kjær, A., Johnston, K., & Köster, U. (2017). Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector. Frontiers in Medicine, 4, [98]. https://doi.org/10.3389/fmed.2017.00098

Vancouver

Severin GW, Kristensen LK, Nielsen CH, Fonslet J, Jensen AI, Frellsen AF o.a. Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector. Frontiers in Medicine. 2017;4. 98. https://doi.org/10.3389/fmed.2017.00098

Author

Severin, Gregory W ; Kristensen, Lotte K ; Nielsen, Carsten H ; Fonslet, Jesper ; Jensen, Andreas I ; Frellsen, Anders F ; Jensen, K M ; Elema, Dennis R ; Maecke, Helmut ; Kjær, Andreas ; Johnston, Karl ; Köster, Ulli. / Neodymium-140 DOTA-LM3 : Evaluation of anGenerator for PET with a Non-Internalizing Vector. I: Frontiers in Medicine. 2017 ; Bind 4.

Bibtex

@article{30e4920c723a4c4188613dc43ae1556f,
title = "Neodymium-140 DOTA-LM3: Evaluation of anGenerator for PET with a Non-Internalizing Vector",
abstract = " 140 Nd (t1/2 = 3.4 days), owing to its short-lived positron emitting daughter140Pr (t1/2 = 3.4 min), has promise as anin vivogenerator for positron emission tomography (PET). However, the electron capture decay of140Nd is chemically disruptive to macrocycle-based radiolabeling, meaning that anin vivoredistribution of the daughter140Pr is expected before positron emission. The purpose of this study was to determine how the delayed positron from the de-labeled140Pr affects preclinical imaging with140Nd. To explore the effect,140Nd was produced at CERN-ISOLDE, reacted with the somatostatin analogue, DOTA-LM3 (1,4,7,10- tetraazacyclododecane, 1,4,7- tri acetic acid, 10- acetamide N - p-Cl-Phecyclo(d-Cys-Tyr-d-4-amino-Phe(carbamoyl)-Lys-Thr-Cys)d-Tyr-NH2) and injected into H727 xenograft bearing mice. Comparative pre- and post-mortem PET imaging at 16 h postinjection was used to quantify thein vivoredistribution of140Pr following140Nd decay. The somatostatin receptor-positive pancreas exhibited the highest tissue accumulation of140Nd-DOTA-LM3 (13% ID/g at 16 h) coupled with the largest observed redistribution rate, where 56 ± 7% (n = 4, mean ± SD) of thein situproduced140Pr washed out of the pancreas before decay. Contrastingly, the liver, spleen, and lungs acted as strong sink organs for free140Pr3+. Based upon these results, we conclude that140Nd imaging with a non-internalizing vector convolutes the biodistribution of the tracer with the accumulation pattern of free140Pr. This redistribution phenomenon may show promise as a probe of the cellular interaction with the vector, such as in determining tissue dependent internalization behavior.",
author = "Severin, {Gregory W} and Kristensen, {Lotte K} and Nielsen, {Carsten H} and Jesper Fonslet and Jensen, {Andreas I} and Frellsen, {Anders F} and Jensen, {K M} and Elema, {Dennis R} and Helmut Maecke and Andreas Kj{\ae}r and Karl Johnston and Ulli K{\"o}ster",
year = "2017",
doi = "10.3389/fmed.2017.00098",
language = "English",
volume = "4",
journal = "Frontiers in Medicine",
issn = "2296-858X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Neodymium-140 DOTA-LM3

T2 - Evaluation of anGenerator for PET with a Non-Internalizing Vector

AU - Severin, Gregory W

AU - Kristensen, Lotte K

AU - Nielsen, Carsten H

AU - Fonslet, Jesper

AU - Jensen, Andreas I

AU - Frellsen, Anders F

AU - Jensen, K M

AU - Elema, Dennis R

AU - Maecke, Helmut

AU - Kjær, Andreas

AU - Johnston, Karl

AU - Köster, Ulli

PY - 2017

Y1 - 2017

N2 - 140 Nd (t1/2 = 3.4 days), owing to its short-lived positron emitting daughter140Pr (t1/2 = 3.4 min), has promise as anin vivogenerator for positron emission tomography (PET). However, the electron capture decay of140Nd is chemically disruptive to macrocycle-based radiolabeling, meaning that anin vivoredistribution of the daughter140Pr is expected before positron emission. The purpose of this study was to determine how the delayed positron from the de-labeled140Pr affects preclinical imaging with140Nd. To explore the effect,140Nd was produced at CERN-ISOLDE, reacted with the somatostatin analogue, DOTA-LM3 (1,4,7,10- tetraazacyclododecane, 1,4,7- tri acetic acid, 10- acetamide N - p-Cl-Phecyclo(d-Cys-Tyr-d-4-amino-Phe(carbamoyl)-Lys-Thr-Cys)d-Tyr-NH2) and injected into H727 xenograft bearing mice. Comparative pre- and post-mortem PET imaging at 16 h postinjection was used to quantify thein vivoredistribution of140Pr following140Nd decay. The somatostatin receptor-positive pancreas exhibited the highest tissue accumulation of140Nd-DOTA-LM3 (13% ID/g at 16 h) coupled with the largest observed redistribution rate, where 56 ± 7% (n = 4, mean ± SD) of thein situproduced140Pr washed out of the pancreas before decay. Contrastingly, the liver, spleen, and lungs acted as strong sink organs for free140Pr3+. Based upon these results, we conclude that140Nd imaging with a non-internalizing vector convolutes the biodistribution of the tracer with the accumulation pattern of free140Pr. This redistribution phenomenon may show promise as a probe of the cellular interaction with the vector, such as in determining tissue dependent internalization behavior.

AB - 140 Nd (t1/2 = 3.4 days), owing to its short-lived positron emitting daughter140Pr (t1/2 = 3.4 min), has promise as anin vivogenerator for positron emission tomography (PET). However, the electron capture decay of140Nd is chemically disruptive to macrocycle-based radiolabeling, meaning that anin vivoredistribution of the daughter140Pr is expected before positron emission. The purpose of this study was to determine how the delayed positron from the de-labeled140Pr affects preclinical imaging with140Nd. To explore the effect,140Nd was produced at CERN-ISOLDE, reacted with the somatostatin analogue, DOTA-LM3 (1,4,7,10- tetraazacyclododecane, 1,4,7- tri acetic acid, 10- acetamide N - p-Cl-Phecyclo(d-Cys-Tyr-d-4-amino-Phe(carbamoyl)-Lys-Thr-Cys)d-Tyr-NH2) and injected into H727 xenograft bearing mice. Comparative pre- and post-mortem PET imaging at 16 h postinjection was used to quantify thein vivoredistribution of140Pr following140Nd decay. The somatostatin receptor-positive pancreas exhibited the highest tissue accumulation of140Nd-DOTA-LM3 (13% ID/g at 16 h) coupled with the largest observed redistribution rate, where 56 ± 7% (n = 4, mean ± SD) of thein situproduced140Pr washed out of the pancreas before decay. Contrastingly, the liver, spleen, and lungs acted as strong sink organs for free140Pr3+. Based upon these results, we conclude that140Nd imaging with a non-internalizing vector convolutes the biodistribution of the tracer with the accumulation pattern of free140Pr. This redistribution phenomenon may show promise as a probe of the cellular interaction with the vector, such as in determining tissue dependent internalization behavior.

U2 - 10.3389/fmed.2017.00098

DO - 10.3389/fmed.2017.00098

M3 - Journal article

C2 - 28748183

VL - 4

JO - Frontiers in Medicine

JF - Frontiers in Medicine

SN - 2296-858X

M1 - 98

ER -

ID: 194529489