Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

  • Nadezda A Brazhe
  • Marek Treiman
  • Alexey R Brazhe
  • Ninett L Find
  • Georgy V Maksimov
  • Sosnovtseva, Olga
This paper presents a nonivasive approach to study redox state of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text]. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes [Formula: see text], [Formula: see text] and [Formula: see text] in the rod-shaped cardiomyocytes caused by H(2)O(2)-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome [Formula: see text] in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.
OriginalsprogEngelsk
TidsskriftP L o S One
Vol/bind7
Udgave nummer9
Sider (fra-til)e41990
ISSN1932-6203
DOI
StatusUdgivet - 2012

ID: 40869572