Lysine demethylase inhibition protects pancreatic β cells from apoptosis and improves β-cell function
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Lysine demethylase inhibition protects pancreatic β cells from apoptosis and improves β-cell function. / Backe, Marie Balslev; Andersson, Jan Legaard; Bacos, Karl; Christensen, Dan Ploug; Hansen, Jakob Bondo; Dorosz, Jerzy Jòzef; Gajhede, Michael; Dahlby, Tina; Bysani, Madhusudhan; Kristensen, Line Hyltoft; Ling, Charlotte; Olsen, Lars; Mandrup-Poulsen, Thomas.
I: Molecular and Cellular Endocrinology, Bind 460, 15.01.2018, s. 47-56.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Lysine demethylase inhibition protects pancreatic β cells from apoptosis and improves β-cell function
AU - Backe, Marie Balslev
AU - Andersson, Jan Legaard
AU - Bacos, Karl
AU - Christensen, Dan Ploug
AU - Hansen, Jakob Bondo
AU - Dorosz, Jerzy Jòzef
AU - Gajhede, Michael
AU - Dahlby, Tina
AU - Bysani, Madhusudhan
AU - Kristensen, Line Hyltoft
AU - Ling, Charlotte
AU - Olsen, Lars
AU - Mandrup-Poulsen, Thomas
N1 - Copyright © 2017. Published by Elsevier B.V.
PY - 2018/1/15
Y1 - 2018/1/15
N2 - Transcriptional changes control β-cell survival in response to inflammatory stress. Posttranslational modifications of histone and non-histone transcriptional regulators activate or repress gene transcription, but the link to cell-fate signaling is unclear. Inhibition of lysine deacetylases (KDACs) protects β cells from cytokine-induced apoptosis and reduces type 1 diabetes incidence in animals. We hypothesized that also lysine demethylases (KDMs) regulate β-cell fate in response to inflammatory stress. Expression of the demethylase Kdm6B was upregulated by proinflammatory cytokines suggesting a possible role in inflammation-induced β-cell destruction. Inhibition of KDM6 demethylases using the selective inhibitor GSK-J4 protected insulin-producing cells and human and mouse islets from cytokine-induced apoptosis by blunting nuclear factor (NF)-κB signaling and endoplasmic reticulum (ER) stress response gene expression. GSK-J4 furthermore increased expression of insulin gene and glucose-stimulated insulin secretion. Expression of genes regulating purinergic and cytokine ligand-receptor interactions was downregulated following GSK-J4 exposure, while expression of genes involved in cell maintenance and survival was upregulated. These data suggest that KDMs are important regulators of inflammation-induced β-cell dysfunction and death.
AB - Transcriptional changes control β-cell survival in response to inflammatory stress. Posttranslational modifications of histone and non-histone transcriptional regulators activate or repress gene transcription, but the link to cell-fate signaling is unclear. Inhibition of lysine deacetylases (KDACs) protects β cells from cytokine-induced apoptosis and reduces type 1 diabetes incidence in animals. We hypothesized that also lysine demethylases (KDMs) regulate β-cell fate in response to inflammatory stress. Expression of the demethylase Kdm6B was upregulated by proinflammatory cytokines suggesting a possible role in inflammation-induced β-cell destruction. Inhibition of KDM6 demethylases using the selective inhibitor GSK-J4 protected insulin-producing cells and human and mouse islets from cytokine-induced apoptosis by blunting nuclear factor (NF)-κB signaling and endoplasmic reticulum (ER) stress response gene expression. GSK-J4 furthermore increased expression of insulin gene and glucose-stimulated insulin secretion. Expression of genes regulating purinergic and cytokine ligand-receptor interactions was downregulated following GSK-J4 exposure, while expression of genes involved in cell maintenance and survival was upregulated. These data suggest that KDMs are important regulators of inflammation-induced β-cell dysfunction and death.
KW - Apoptosis
KW - beta cells
KW - Inflammation
KW - Lysine demethylases
KW - Gene expression
KW - Diabetes
U2 - 10.1016/j.mce.2017.07.001
DO - 10.1016/j.mce.2017.07.001
M3 - Journal article
C2 - 28684291
VL - 460
SP - 47
EP - 56
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
ER -
ID: 187554869