Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture

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Standard

Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture. / Brunstedt, J; Nielsen, Jens Høiriis.

I: Diabetologia, Bind 15, Nr. 3, 09.1978, s. 181-5.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Brunstedt, J & Nielsen, JH 1978, 'Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture', Diabetologia, bind 15, nr. 3, s. 181-5.

APA

Brunstedt, J., & Nielsen, J. H. (1978). Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture. Diabetologia, 15(3), 181-5.

Vancouver

Brunstedt J, Nielsen JH. Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture. Diabetologia. 1978 sep.;15(3):181-5.

Author

Brunstedt, J ; Nielsen, Jens Høiriis. / Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture. I: Diabetologia. 1978 ; Bind 15, Nr. 3. s. 181-5.

Bibtex

@article{76d7291e737649ef8cbccbc8ba1b4915,
title = "Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture",
abstract = "Collagenase isolated mouse pancreatic islets were maintained in tissue culture for up to 5 months in a culture medium buffered with Hepes and the pH varying between 6.8 and 7.6. The amount of insulin released into the medium and the insulin response to glucose and glucose plus theophylline were measured during the culture period. It was found that islets cultured at pH 7.2 maintained the ability to release insulin into the medium for at least 5 months, which was longer than islets cultured at the other pH values. During the first weeks, the islets cultured at pH 7.6 had a higher response to both glucose and glucose plus theophylline than islets cultured at the other pH values, but later they lost their insulin releasing ability.",
keywords = "Animals, Culture Media, Culture Techniques, Glucose, Hydrogen-Ion Concentration, Insulin, Islets of Langerhans, Male, Mice, Theophylline, Time Factors",
author = "J Brunstedt and Nielsen, {Jens H{\o}iriis}",
year = "1978",
month = sep,
language = "English",
volume = "15",
pages = "181--5",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture

AU - Brunstedt, J

AU - Nielsen, Jens Høiriis

PY - 1978/9

Y1 - 1978/9

N2 - Collagenase isolated mouse pancreatic islets were maintained in tissue culture for up to 5 months in a culture medium buffered with Hepes and the pH varying between 6.8 and 7.6. The amount of insulin released into the medium and the insulin response to glucose and glucose plus theophylline were measured during the culture period. It was found that islets cultured at pH 7.2 maintained the ability to release insulin into the medium for at least 5 months, which was longer than islets cultured at the other pH values. During the first weeks, the islets cultured at pH 7.6 had a higher response to both glucose and glucose plus theophylline than islets cultured at the other pH values, but later they lost their insulin releasing ability.

AB - Collagenase isolated mouse pancreatic islets were maintained in tissue culture for up to 5 months in a culture medium buffered with Hepes and the pH varying between 6.8 and 7.6. The amount of insulin released into the medium and the insulin response to glucose and glucose plus theophylline were measured during the culture period. It was found that islets cultured at pH 7.2 maintained the ability to release insulin into the medium for at least 5 months, which was longer than islets cultured at the other pH values. During the first weeks, the islets cultured at pH 7.6 had a higher response to both glucose and glucose plus theophylline than islets cultured at the other pH values, but later they lost their insulin releasing ability.

KW - Animals

KW - Culture Media

KW - Culture Techniques

KW - Glucose

KW - Hydrogen-Ion Concentration

KW - Insulin

KW - Islets of Langerhans

KW - Male

KW - Mice

KW - Theophylline

KW - Time Factors

M3 - Journal article

C2 - 29815

VL - 15

SP - 181

EP - 185

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 3

ER -

ID: 47975650