Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. / Spinas, G A; Hansen, B S; Linde, S; Kastern, W; Mølvig, J; Mandrup-Poulsen, T; Dinarello, C A; Nerup, J; Nielsen, Jens Høiriis.

I: Diabetologia, Bind 30, Nr. 7, 07.1987, s. 474-80.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Spinas, GA, Hansen, BS, Linde, S, Kastern, W, Mølvig, J, Mandrup-Poulsen, T, Dinarello, CA, Nerup, J & Nielsen, JH 1987, 'Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans', Diabetologia, bind 30, nr. 7, s. 474-80.

APA

Spinas, G. A., Hansen, B. S., Linde, S., Kastern, W., Mølvig, J., Mandrup-Poulsen, T., ... Nielsen, J. H. (1987). Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. Diabetologia, 30(7), 474-80.

Vancouver

Spinas GA, Hansen BS, Linde S, Kastern W, Mølvig J, Mandrup-Poulsen T o.a. Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. Diabetologia. 1987 jul;30(7):474-80.

Author

Spinas, G A ; Hansen, B S ; Linde, S ; Kastern, W ; Mølvig, J ; Mandrup-Poulsen, T ; Dinarello, C A ; Nerup, J ; Nielsen, Jens Høiriis. / Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. I: Diabetologia. 1987 ; Bind 30, Nr. 7. s. 474-80.

Bibtex

@article{8b924cfea7834cd4bc3b70f54fb5007b,
title = "Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans",
abstract = "Human crude and recombinant interleukin 1 (IL-1) was found to dose- and time-dependently affect the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. Incubation of rat islets with either 0.5 U/ml or 5 U/ml of crude IL-1 for 1 h had no detectable effect on (pro)insulin biosynthesis. After 24 hours of exposure 0.5 U/ml of crude or 0.6 ng/ml of recombinant IL-1 (beta) increased the (pro)insulin biosynthesis by 42{\%} and 58{\%}, respectively, whereas a 10-fold greater concentration of IL-1 decreased the (pro)insulin biosynthesis by 74{\%} and 89{\%}, respectively. The increase in (pro)insulin biosynthesis was accompanied by an increase in total protein biosynthesis indicating a nonspecific stimulatory action of low IL-1 concentrations. In contrast, high IL-1 concentrations caused a more selective decrease of the (pro) insulin biosynthesis when compared to the total protein biosynthesis. In addition, low IL-1 concentrations were found to increase and high concentrations to decrease the relative levels of pre-proinsulin mRNA suggesting that IL-1 may act both at a pre- and post-translational level of insulin biosynthesis.",
keywords = "Animals, Chromatography, High Pressure Liquid, Insulin, Interleukin-1, Islets of Langerhans, Proinsulin, Protein Biosynthesis, Protein Precursors, RNA, Messenger, Rats, Rats, Inbred Strains",
author = "Spinas, {G A} and Hansen, {B S} and S Linde and W Kastern and J M{\o}lvig and T Mandrup-Poulsen and Dinarello, {C A} and J Nerup and Nielsen, {Jens H{\o}iriis}",
year = "1987",
month = "7",
language = "English",
volume = "30",
pages = "474--80",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer",
number = "7",

}

RIS

TY - JOUR

T1 - Interleukin 1 dose-dependently affects the biosynthesis of (pro)insulin in isolated rat islets of Langerhans

AU - Spinas, G A

AU - Hansen, B S

AU - Linde, S

AU - Kastern, W

AU - Mølvig, J

AU - Mandrup-Poulsen, T

AU - Dinarello, C A

AU - Nerup, J

AU - Nielsen, Jens Høiriis

PY - 1987/7

Y1 - 1987/7

N2 - Human crude and recombinant interleukin 1 (IL-1) was found to dose- and time-dependently affect the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. Incubation of rat islets with either 0.5 U/ml or 5 U/ml of crude IL-1 for 1 h had no detectable effect on (pro)insulin biosynthesis. After 24 hours of exposure 0.5 U/ml of crude or 0.6 ng/ml of recombinant IL-1 (beta) increased the (pro)insulin biosynthesis by 42% and 58%, respectively, whereas a 10-fold greater concentration of IL-1 decreased the (pro)insulin biosynthesis by 74% and 89%, respectively. The increase in (pro)insulin biosynthesis was accompanied by an increase in total protein biosynthesis indicating a nonspecific stimulatory action of low IL-1 concentrations. In contrast, high IL-1 concentrations caused a more selective decrease of the (pro) insulin biosynthesis when compared to the total protein biosynthesis. In addition, low IL-1 concentrations were found to increase and high concentrations to decrease the relative levels of pre-proinsulin mRNA suggesting that IL-1 may act both at a pre- and post-translational level of insulin biosynthesis.

AB - Human crude and recombinant interleukin 1 (IL-1) was found to dose- and time-dependently affect the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. Incubation of rat islets with either 0.5 U/ml or 5 U/ml of crude IL-1 for 1 h had no detectable effect on (pro)insulin biosynthesis. After 24 hours of exposure 0.5 U/ml of crude or 0.6 ng/ml of recombinant IL-1 (beta) increased the (pro)insulin biosynthesis by 42% and 58%, respectively, whereas a 10-fold greater concentration of IL-1 decreased the (pro)insulin biosynthesis by 74% and 89%, respectively. The increase in (pro)insulin biosynthesis was accompanied by an increase in total protein biosynthesis indicating a nonspecific stimulatory action of low IL-1 concentrations. In contrast, high IL-1 concentrations caused a more selective decrease of the (pro) insulin biosynthesis when compared to the total protein biosynthesis. In addition, low IL-1 concentrations were found to increase and high concentrations to decrease the relative levels of pre-proinsulin mRNA suggesting that IL-1 may act both at a pre- and post-translational level of insulin biosynthesis.

KW - Animals

KW - Chromatography, High Pressure Liquid

KW - Insulin

KW - Interleukin-1

KW - Islets of Langerhans

KW - Proinsulin

KW - Protein Biosynthesis

KW - Protein Precursors

KW - RNA, Messenger

KW - Rats

KW - Rats, Inbred Strains

M3 - Journal article

C2 - 3311860

VL - 30

SP - 474

EP - 480

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 7

ER -

ID: 47974849