Influence of pre-exercise muscle glycogen content on exercise-induced transcriptional regulation of metabolic genes.

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Standard

Influence of pre-exercise muscle glycogen content on exercise-induced transcriptional regulation of metabolic genes. / Pilegaard, Henriette; Keller, Charlotte; Steensberg, Adam; Helge, Jørn Wulff; Pedersen, Bente Klarlund; Saltin, Bengt; Neufer, P Darrell.

I: Journal of Physiology, Bind 541, Nr. Pt 1, 2002, s. 261-71.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Pilegaard, H, Keller, C, Steensberg, A, Helge, JW, Pedersen, BK, Saltin, B & Neufer, PD 2002, 'Influence of pre-exercise muscle glycogen content on exercise-induced transcriptional regulation of metabolic genes.', Journal of Physiology, bind 541, nr. Pt 1, s. 261-71.

APA

Pilegaard, H., Keller, C., Steensberg, A., Helge, J. W., Pedersen, B. K., Saltin, B., & Neufer, P. D. (2002). Influence of pre-exercise muscle glycogen content on exercise-induced transcriptional regulation of metabolic genes. Journal of Physiology, 541(Pt 1), 261-71.

Vancouver

Pilegaard H, Keller C, Steensberg A, Helge JW, Pedersen BK, Saltin B o.a. Influence of pre-exercise muscle glycogen content on exercise-induced transcriptional regulation of metabolic genes. Journal of Physiology. 2002;541(Pt 1):261-71.

Author

Pilegaard, Henriette ; Keller, Charlotte ; Steensberg, Adam ; Helge, Jørn Wulff ; Pedersen, Bente Klarlund ; Saltin, Bengt ; Neufer, P Darrell. / Influence of pre-exercise muscle glycogen content on exercise-induced transcriptional regulation of metabolic genes. I: Journal of Physiology. 2002 ; Bind 541, Nr. Pt 1. s. 261-71.

Bibtex

@article{b888ce50ab5b11ddb5e9000ea68e967b,
title = "Influence of pre-exercise muscle glycogen content on exercise-induced transcriptional regulation of metabolic genes.",
abstract = "Transcription of metabolic genes is transiently induced during recovery from exercise in skeletal muscle of humans. To determine whether pre-exercise muscle glycogen content influences the magnitude and/or duration of this adaptive response, six male subjects performed one-legged cycling exercise to lower muscle glycogen content in one leg and then, the following day, completed 2.5 h low intensity two-legged cycling exercise. Nuclei and mRNA were isolated from biopsies obtained from the vastus lateralis muscle of the control and reduced glycogen (pre-exercise glycogen = 609 +/- 47 and 337 +/- 33 mmol kg(-1) dry weight, respectively) legs before and after 0, 2 and 5 h of recovery. Exercise induced a significant (P <0.05) ( (2- (240 (398 (hkii) (lpl) (pdk4) (separated (ucp3) + - 2 3 3-fold) 38 4 52 a although and before between by completed consistent consisting control dehydrogenase difference dry either elevated exercise exercise, exercise. extensor found genes glycogen glycogen. greater h hexokinase ii in increase induced kg(-1) kinase knee leg legs lipase lipoprotein low mmol mrna muscle no of only. or pdk4 pdk4, pre-exercise protein pyruvate reduced response second significantly six study, subjects the to transcription trials two two-legged uncoupling was weeks) weight) were with> 6-fold) than in the control (< 3-fold) trial. Induction of PDK4 and UCP3 mRNA in response to exercise was also significantly higher in the low glycogen (11.4- and 3.5-fold, respectively) than in the control (5.0- and 1.7-fold, respectively) trial. These data indicate that low muscle glycogen content enhances the transcriptional activation of some metabolic genes in response to exercise, raising the possibility that signalling mechanisms sensitive to glycogen content and/or FFA availability may be linked to the transcriptional control of exercise-responsive genes.",
author = "Henriette Pilegaard and Charlotte Keller and Adam Steensberg and Helge, {J{\o}rn Wulff} and Pedersen, {Bente Klarlund} and Bengt Saltin and Neufer, {P Darrell}",
note = "Keywords: Adult; Bicycling; Blood Glucose; Cell Nucleus; DNA; Dietary Carbohydrates; Exercise; Fatty Acids, Nonesterified; Gene Expression Regulation; Glycogen; Humans; Leg; Male; Metabolism; Muscle, Skeletal; Oxygen Consumption; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction",
year = "2002",
language = "English",
volume = "541",
pages = "261--71",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "Pt 1",

}

RIS

TY - JOUR

T1 - Influence of pre-exercise muscle glycogen content on exercise-induced transcriptional regulation of metabolic genes.

AU - Pilegaard, Henriette

AU - Keller, Charlotte

AU - Steensberg, Adam

AU - Helge, Jørn Wulff

AU - Pedersen, Bente Klarlund

AU - Saltin, Bengt

AU - Neufer, P Darrell

N1 - Keywords: Adult; Bicycling; Blood Glucose; Cell Nucleus; DNA; Dietary Carbohydrates; Exercise; Fatty Acids, Nonesterified; Gene Expression Regulation; Glycogen; Humans; Leg; Male; Metabolism; Muscle, Skeletal; Oxygen Consumption; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction

PY - 2002

Y1 - 2002

N2 - Transcription of metabolic genes is transiently induced during recovery from exercise in skeletal muscle of humans. To determine whether pre-exercise muscle glycogen content influences the magnitude and/or duration of this adaptive response, six male subjects performed one-legged cycling exercise to lower muscle glycogen content in one leg and then, the following day, completed 2.5 h low intensity two-legged cycling exercise. Nuclei and mRNA were isolated from biopsies obtained from the vastus lateralis muscle of the control and reduced glycogen (pre-exercise glycogen = 609 +/- 47 and 337 +/- 33 mmol kg(-1) dry weight, respectively) legs before and after 0, 2 and 5 h of recovery. Exercise induced a significant (P <0.05) ( (2- (240 (398 (hkii) (lpl) (pdk4) (separated (ucp3) + - 2 3 3-fold) 38 4 52 a although and before between by completed consistent consisting control dehydrogenase difference dry either elevated exercise exercise, exercise. extensor found genes glycogen glycogen. greater h hexokinase ii in increase induced kg(-1) kinase knee leg legs lipase lipoprotein low mmol mrna muscle no of only. or pdk4 pdk4, pre-exercise protein pyruvate reduced response second significantly six study, subjects the to transcription trials two two-legged uncoupling was weeks) weight) were with> 6-fold) than in the control (< 3-fold) trial. Induction of PDK4 and UCP3 mRNA in response to exercise was also significantly higher in the low glycogen (11.4- and 3.5-fold, respectively) than in the control (5.0- and 1.7-fold, respectively) trial. These data indicate that low muscle glycogen content enhances the transcriptional activation of some metabolic genes in response to exercise, raising the possibility that signalling mechanisms sensitive to glycogen content and/or FFA availability may be linked to the transcriptional control of exercise-responsive genes.

AB - Transcription of metabolic genes is transiently induced during recovery from exercise in skeletal muscle of humans. To determine whether pre-exercise muscle glycogen content influences the magnitude and/or duration of this adaptive response, six male subjects performed one-legged cycling exercise to lower muscle glycogen content in one leg and then, the following day, completed 2.5 h low intensity two-legged cycling exercise. Nuclei and mRNA were isolated from biopsies obtained from the vastus lateralis muscle of the control and reduced glycogen (pre-exercise glycogen = 609 +/- 47 and 337 +/- 33 mmol kg(-1) dry weight, respectively) legs before and after 0, 2 and 5 h of recovery. Exercise induced a significant (P <0.05) ( (2- (240 (398 (hkii) (lpl) (pdk4) (separated (ucp3) + - 2 3 3-fold) 38 4 52 a although and before between by completed consistent consisting control dehydrogenase difference dry either elevated exercise exercise, exercise. extensor found genes glycogen glycogen. greater h hexokinase ii in increase induced kg(-1) kinase knee leg legs lipase lipoprotein low mmol mrna muscle no of only. or pdk4 pdk4, pre-exercise protein pyruvate reduced response second significantly six study, subjects the to transcription trials two two-legged uncoupling was weeks) weight) were with> 6-fold) than in the control (< 3-fold) trial. Induction of PDK4 and UCP3 mRNA in response to exercise was also significantly higher in the low glycogen (11.4- and 3.5-fold, respectively) than in the control (5.0- and 1.7-fold, respectively) trial. These data indicate that low muscle glycogen content enhances the transcriptional activation of some metabolic genes in response to exercise, raising the possibility that signalling mechanisms sensitive to glycogen content and/or FFA availability may be linked to the transcriptional control of exercise-responsive genes.

M3 - Journal article

C2 - 12015434

VL - 541

SP - 261

EP - 271

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - Pt 1

ER -

ID: 8419569