Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells

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Standard

Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells. / Anisimov, Sergey V.; Christophersen, Nicolaj S.; Correia, Ana S.; Hall, Vanessa Jane; Sandeling, Ingrid; Li, Jia-Yi; Brundin, Patrik.

I: Cellular & Molecular Biology Letters, Bind 16, Nr. 1, 2011, s. 79-88.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Anisimov, SV, Christophersen, NS, Correia, AS, Hall, VJ, Sandeling, I, Li, J-Y & Brundin, P 2011, 'Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells', Cellular & Molecular Biology Letters, bind 16, nr. 1, s. 79-88. https://doi.org/10.2478/s11658-010-0039-8

APA

Anisimov, S. V., Christophersen, N. S., Correia, A. S., Hall, V. J., Sandeling, I., Li, J-Y., & Brundin, P. (2011). Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells. Cellular & Molecular Biology Letters, 16(1), 79-88. https://doi.org/10.2478/s11658-010-0039-8

Vancouver

Anisimov SV, Christophersen NS, Correia AS, Hall VJ, Sandeling I, Li J-Y o.a. Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells. Cellular & Molecular Biology Letters. 2011;16(1):79-88. https://doi.org/10.2478/s11658-010-0039-8

Author

Anisimov, Sergey V. ; Christophersen, Nicolaj S. ; Correia, Ana S. ; Hall, Vanessa Jane ; Sandeling, Ingrid ; Li, Jia-Yi ; Brundin, Patrik. / Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells. I: Cellular & Molecular Biology Letters. 2011 ; Bind 16, Nr. 1. s. 79-88.

Bibtex

@article{e4294dfac37048f49f124cdcefbbfb2d,
title = "Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells",
abstract = "The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.",
keywords = "Former LIFE faculty, Human embryonic stem cells, Feeder cells, DNA microarray",
author = "Anisimov, {Sergey V.} and Christophersen, {Nicolaj S.} and Correia, {Ana S.} and Hall, {Vanessa Jane} and Ingrid Sandeling and Jia-Yi Li and Patrik Brundin",
year = "2011",
doi = "10.2478/s11658-010-0039-8",
language = "English",
volume = "16",
pages = "79--88",
journal = "Cellular & Molecular Biology Letters",
issn = "1425-8153",
publisher = "Versita",
number = "1",

}

RIS

TY - JOUR

T1 - Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells

AU - Anisimov, Sergey V.

AU - Christophersen, Nicolaj S.

AU - Correia, Ana S.

AU - Hall, Vanessa Jane

AU - Sandeling, Ingrid

AU - Li, Jia-Yi

AU - Brundin, Patrik

PY - 2011

Y1 - 2011

N2 - The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.

AB - The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.

KW - Former LIFE faculty

KW - Human embryonic stem cells

KW - Feeder cells

KW - DNA microarray

U2 - 10.2478/s11658-010-0039-8

DO - 10.2478/s11658-010-0039-8

M3 - Journal article

C2 - 21161417

VL - 16

SP - 79

EP - 88

JO - Cellular & Molecular Biology Letters

JF - Cellular & Molecular Biology Letters

SN - 1425-8153

IS - 1

ER -

ID: 32438093