Hypoxia-Inducible Factor-1α Expression in Macrophages Promotes Development of Atherosclerosis

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Standard

Hypoxia-Inducible Factor-1α Expression in Macrophages Promotes Development of Atherosclerosis. / Pedersen, Annemarie Aarup; Pedersen, Tanja X; Junker, Nanna ; Christoffersen, Christina; Bartels, Emil D; Madsen, Marie; Nielsen, Carsten H; Nielsen, Lars Bo.

I: Arteriosclerosis, Thrombosis, and Vascular Biology, Bind 36, Nr. 9, 09.2016, s. 1782-1790.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Pedersen, AA, Pedersen, TX, Junker, N, Christoffersen, C, Bartels, ED, Madsen, M, Nielsen, CH & Nielsen, LB 2016, 'Hypoxia-Inducible Factor-1α Expression in Macrophages Promotes Development of Atherosclerosis', Arteriosclerosis, Thrombosis, and Vascular Biology, bind 36, nr. 9, s. 1782-1790. https://doi.org/10.1161/ATVBAHA.116.307830

APA

Pedersen, A. A., Pedersen, T. X., Junker, N., Christoffersen, C., Bartels, E. D., Madsen, M., Nielsen, C. H., & Nielsen, L. B. (2016). Hypoxia-Inducible Factor-1α Expression in Macrophages Promotes Development of Atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 36(9), 1782-1790. https://doi.org/10.1161/ATVBAHA.116.307830

Vancouver

Pedersen AA, Pedersen TX, Junker N, Christoffersen C, Bartels ED, Madsen M o.a. Hypoxia-Inducible Factor-1α Expression in Macrophages Promotes Development of Atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology. 2016 sep.;36(9):1782-1790. https://doi.org/10.1161/ATVBAHA.116.307830

Author

Pedersen, Annemarie Aarup ; Pedersen, Tanja X ; Junker, Nanna ; Christoffersen, Christina ; Bartels, Emil D ; Madsen, Marie ; Nielsen, Carsten H ; Nielsen, Lars Bo. / Hypoxia-Inducible Factor-1α Expression in Macrophages Promotes Development of Atherosclerosis. I: Arteriosclerosis, Thrombosis, and Vascular Biology. 2016 ; Bind 36, Nr. 9. s. 1782-1790.

Bibtex

@article{10c0a268f67c4b7dab98779d293fbfb2,
title = "Hypoxia-Inducible Factor-1α Expression in Macrophages Promotes Development of Atherosclerosis",
abstract = "OBJECTIVE: Atherosclerotic lesions contain hypoxic areas, but the pathophysiological importance of hypoxia is unknown. Hypoxia-inducible factor-1α (HIF-1α) is a key transcription factor in cellular responses to hypoxia. We investigated the hypothesis that HIF-1α has effects on macrophage biology that promotes atherogenesis in mice.APPROACH AND RESULTS: Studies with molecular probes, immunostaining, and laser microdissection of aortas revealed abundant hypoxic, HIF-1α-expressing macrophages in murine atherosclerotic lesions. To investigate the significance of macrophage HIF-1α, Ldlr(-/-) mice were transplanted with bone marrow from mice with HIF-1α deficiency in the myeloid cells or control bone marrow. The HIF-1α deficiency in myeloid cells reduced atherosclerosis in aorta of the Ldlr(-/-) recipient mice by ≈72% (P=0.006).In vitro, HIF-1α-deficient macrophages displayed decreased differentiation to proinflammatory M1 macrophages and reduced expression of inflammatory genes. HIF-1α deficiency also affected glucose uptake, apoptosis, and migratory abilities of the macrophages.CONCLUSIONS: HIF-1α expression in macrophages affects their intrinsic inflammatory profile and promotes development of atherosclerosis.",
author = "Pedersen, {Annemarie Aarup} and Pedersen, {Tanja X} and Nanna Junker and Christina Christoffersen and Bartels, {Emil D} and Marie Madsen and Nielsen, {Carsten H} and Nielsen, {Lars Bo}",
note = "{\textcopyright} 2016 American Heart Association, Inc.",
year = "2016",
month = sep,
doi = "10.1161/ATVBAHA.116.307830",
language = "English",
volume = "36",
pages = "1782--1790",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams & Wilkins",
number = "9",

}

RIS

TY - JOUR

T1 - Hypoxia-Inducible Factor-1α Expression in Macrophages Promotes Development of Atherosclerosis

AU - Pedersen, Annemarie Aarup

AU - Pedersen, Tanja X

AU - Junker, Nanna

AU - Christoffersen, Christina

AU - Bartels, Emil D

AU - Madsen, Marie

AU - Nielsen, Carsten H

AU - Nielsen, Lars Bo

N1 - © 2016 American Heart Association, Inc.

PY - 2016/9

Y1 - 2016/9

N2 - OBJECTIVE: Atherosclerotic lesions contain hypoxic areas, but the pathophysiological importance of hypoxia is unknown. Hypoxia-inducible factor-1α (HIF-1α) is a key transcription factor in cellular responses to hypoxia. We investigated the hypothesis that HIF-1α has effects on macrophage biology that promotes atherogenesis in mice.APPROACH AND RESULTS: Studies with molecular probes, immunostaining, and laser microdissection of aortas revealed abundant hypoxic, HIF-1α-expressing macrophages in murine atherosclerotic lesions. To investigate the significance of macrophage HIF-1α, Ldlr(-/-) mice were transplanted with bone marrow from mice with HIF-1α deficiency in the myeloid cells or control bone marrow. The HIF-1α deficiency in myeloid cells reduced atherosclerosis in aorta of the Ldlr(-/-) recipient mice by ≈72% (P=0.006).In vitro, HIF-1α-deficient macrophages displayed decreased differentiation to proinflammatory M1 macrophages and reduced expression of inflammatory genes. HIF-1α deficiency also affected glucose uptake, apoptosis, and migratory abilities of the macrophages.CONCLUSIONS: HIF-1α expression in macrophages affects their intrinsic inflammatory profile and promotes development of atherosclerosis.

AB - OBJECTIVE: Atherosclerotic lesions contain hypoxic areas, but the pathophysiological importance of hypoxia is unknown. Hypoxia-inducible factor-1α (HIF-1α) is a key transcription factor in cellular responses to hypoxia. We investigated the hypothesis that HIF-1α has effects on macrophage biology that promotes atherogenesis in mice.APPROACH AND RESULTS: Studies with molecular probes, immunostaining, and laser microdissection of aortas revealed abundant hypoxic, HIF-1α-expressing macrophages in murine atherosclerotic lesions. To investigate the significance of macrophage HIF-1α, Ldlr(-/-) mice were transplanted with bone marrow from mice with HIF-1α deficiency in the myeloid cells or control bone marrow. The HIF-1α deficiency in myeloid cells reduced atherosclerosis in aorta of the Ldlr(-/-) recipient mice by ≈72% (P=0.006).In vitro, HIF-1α-deficient macrophages displayed decreased differentiation to proinflammatory M1 macrophages and reduced expression of inflammatory genes. HIF-1α deficiency also affected glucose uptake, apoptosis, and migratory abilities of the macrophages.CONCLUSIONS: HIF-1α expression in macrophages affects their intrinsic inflammatory profile and promotes development of atherosclerosis.

U2 - 10.1161/ATVBAHA.116.307830

DO - 10.1161/ATVBAHA.116.307830

M3 - Journal article

C2 - 27444197

VL - 36

SP - 1782

EP - 1790

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 9

ER -

ID: 172616766