Human skeletal muscle fibroblasts stimulate in vitro myogenesis and in vivo muscle regeneration

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Standard

Human skeletal muscle fibroblasts stimulate in vitro myogenesis and in vivo muscle regeneration. / Mackey, Abigail L.; Magnan, Mélanie; Chazaud, Bénédicte; Kjaer, Michael.

I: The Journal of Physiology, Bind 595, Nr. 15, 2017, s. 5115-5127.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Mackey, AL, Magnan, M, Chazaud, B & Kjaer, M 2017, 'Human skeletal muscle fibroblasts stimulate in vitro myogenesis and in vivo muscle regeneration', The Journal of Physiology, bind 595, nr. 15, s. 5115-5127. https://doi.org/10.1113/JP273997

APA

Mackey, A. L., Magnan, M., Chazaud, B., & Kjaer, M. (2017). Human skeletal muscle fibroblasts stimulate in vitro myogenesis and in vivo muscle regeneration. The Journal of Physiology, 595(15), 5115-5127. https://doi.org/10.1113/JP273997

Vancouver

Mackey AL, Magnan M, Chazaud B, Kjaer M. Human skeletal muscle fibroblasts stimulate in vitro myogenesis and in vivo muscle regeneration. The Journal of Physiology. 2017;595(15):5115-5127. https://doi.org/10.1113/JP273997

Author

Mackey, Abigail L. ; Magnan, Mélanie ; Chazaud, Bénédicte ; Kjaer, Michael. / Human skeletal muscle fibroblasts stimulate in vitro myogenesis and in vivo muscle regeneration. I: The Journal of Physiology. 2017 ; Bind 595, Nr. 15. s. 5115-5127.

Bibtex

@article{d9e0c63708694c0d8845262de1207c5b,
title = "Human skeletal muscle fibroblasts stimulate in vitro myogenesis and in vivo muscle regeneration",
abstract = "Accumulation of skeletal muscle extracellular matrix is an unfavourable characteristic of many muscle diseases, muscle injury and sarcopenia. In addition to the indispensable role satellite cells play in muscle regeneration, there is emerging evidence in rodents for a regulatory influence on fibroblast activity. However, the influence of fibroblasts on satellite cells and muscle regeneration in humans is unknown. The purpose of this study was to investigate this in vitro and during in vivo regeneration in humans. Following a muscle injury protocol in young healthy men (n = 7), the number of fibroblasts (TCF7L2+), satellite cells (Pax7+), differentiating myogenic cells (myogenin+) and regenerating fibres (neonatal/embryonic myosin+) was determined from biopsy cross-sections. Fibroblasts and myogenic precursor cells (MPCs) were also isolated from human skeletal muscle (n = 4) and co-cultured using different cell ratios, with the two cell populations either in direct contact with each other or separated by a permeable membrane. MPC proliferation, differentiation and fusion were assessed from cells stained for BrdU, desmin and myogenin. On biopsy cross-sections, fibroblast number was seen to increase, along with myogenic cell number, by d7 and increase further by d30, where fibroblasts were observed to be preferentially located immediately surrounding regenerating muscle fibres. In vitro, the presence of fibroblasts in direct contact with MPCs was found to moderately stimulate MPC proliferation and strongly stimulate both MPC differentiation and MPC fusion. It thus appears, in humans, that fibroblasts exert a strong positive regulatory influence on MPC activity, in line with observations during in vivo skeletal muscle regeneration.",
author = "Mackey, {Abigail L.} and M{\'e}lanie Magnan and B{\'e}n{\'e}dicte Chazaud and Michael Kjaer",
year = "2017",
doi = "10.1113/JP273997",
language = "English",
volume = "595",
pages = "5115--5127",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "15",

}

RIS

TY - JOUR

T1 - Human skeletal muscle fibroblasts stimulate in vitro myogenesis and in vivo muscle regeneration

AU - Mackey, Abigail L.

AU - Magnan, Mélanie

AU - Chazaud, Bénédicte

AU - Kjaer, Michael

PY - 2017

Y1 - 2017

N2 - Accumulation of skeletal muscle extracellular matrix is an unfavourable characteristic of many muscle diseases, muscle injury and sarcopenia. In addition to the indispensable role satellite cells play in muscle regeneration, there is emerging evidence in rodents for a regulatory influence on fibroblast activity. However, the influence of fibroblasts on satellite cells and muscle regeneration in humans is unknown. The purpose of this study was to investigate this in vitro and during in vivo regeneration in humans. Following a muscle injury protocol in young healthy men (n = 7), the number of fibroblasts (TCF7L2+), satellite cells (Pax7+), differentiating myogenic cells (myogenin+) and regenerating fibres (neonatal/embryonic myosin+) was determined from biopsy cross-sections. Fibroblasts and myogenic precursor cells (MPCs) were also isolated from human skeletal muscle (n = 4) and co-cultured using different cell ratios, with the two cell populations either in direct contact with each other or separated by a permeable membrane. MPC proliferation, differentiation and fusion were assessed from cells stained for BrdU, desmin and myogenin. On biopsy cross-sections, fibroblast number was seen to increase, along with myogenic cell number, by d7 and increase further by d30, where fibroblasts were observed to be preferentially located immediately surrounding regenerating muscle fibres. In vitro, the presence of fibroblasts in direct contact with MPCs was found to moderately stimulate MPC proliferation and strongly stimulate both MPC differentiation and MPC fusion. It thus appears, in humans, that fibroblasts exert a strong positive regulatory influence on MPC activity, in line with observations during in vivo skeletal muscle regeneration.

AB - Accumulation of skeletal muscle extracellular matrix is an unfavourable characteristic of many muscle diseases, muscle injury and sarcopenia. In addition to the indispensable role satellite cells play in muscle regeneration, there is emerging evidence in rodents for a regulatory influence on fibroblast activity. However, the influence of fibroblasts on satellite cells and muscle regeneration in humans is unknown. The purpose of this study was to investigate this in vitro and during in vivo regeneration in humans. Following a muscle injury protocol in young healthy men (n = 7), the number of fibroblasts (TCF7L2+), satellite cells (Pax7+), differentiating myogenic cells (myogenin+) and regenerating fibres (neonatal/embryonic myosin+) was determined from biopsy cross-sections. Fibroblasts and myogenic precursor cells (MPCs) were also isolated from human skeletal muscle (n = 4) and co-cultured using different cell ratios, with the two cell populations either in direct contact with each other or separated by a permeable membrane. MPC proliferation, differentiation and fusion were assessed from cells stained for BrdU, desmin and myogenin. On biopsy cross-sections, fibroblast number was seen to increase, along with myogenic cell number, by d7 and increase further by d30, where fibroblasts were observed to be preferentially located immediately surrounding regenerating muscle fibres. In vitro, the presence of fibroblasts in direct contact with MPCs was found to moderately stimulate MPC proliferation and strongly stimulate both MPC differentiation and MPC fusion. It thus appears, in humans, that fibroblasts exert a strong positive regulatory influence on MPC activity, in line with observations during in vivo skeletal muscle regeneration.

U2 - 10.1113/JP273997

DO - 10.1113/JP273997

M3 - Journal article

C2 - 28369879

VL - 595

SP - 5115

EP - 5127

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 15

ER -

ID: 174702546