High-intensity exercise training alters the effect of N-acetylcysteine on exercise-related muscle ionic shifts in men

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Standard

High-intensity exercise training alters the effect of N-acetylcysteine on exercise-related muscle ionic shifts in men. / Lemminger, Anders Krogh; Fiorenza, Matteo; Eibye, Kasper; Bangsbo, Jens; Hostrup, Morten.

I: Antioxidants, Bind 12, Nr. 1, 53, 2023.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Lemminger, AK, Fiorenza, M, Eibye, K, Bangsbo, J & Hostrup, M 2023, 'High-intensity exercise training alters the effect of N-acetylcysteine on exercise-related muscle ionic shifts in men', Antioxidants, bind 12, nr. 1, 53. https://doi.org/10.3390/antiox12010053

APA

Lemminger, A. K., Fiorenza, M., Eibye, K., Bangsbo, J., & Hostrup, M. (2023). High-intensity exercise training alters the effect of N-acetylcysteine on exercise-related muscle ionic shifts in men. Antioxidants, 12(1), [53]. https://doi.org/10.3390/antiox12010053

Vancouver

Lemminger AK, Fiorenza M, Eibye K, Bangsbo J, Hostrup M. High-intensity exercise training alters the effect of N-acetylcysteine on exercise-related muscle ionic shifts in men. Antioxidants. 2023;12(1). 53. https://doi.org/10.3390/antiox12010053

Author

Lemminger, Anders Krogh ; Fiorenza, Matteo ; Eibye, Kasper ; Bangsbo, Jens ; Hostrup, Morten. / High-intensity exercise training alters the effect of N-acetylcysteine on exercise-related muscle ionic shifts in men. I: Antioxidants. 2023 ; Bind 12, Nr. 1.

Bibtex

@article{aada7b7bca174b87971a17d86456eb1b,
title = "High-intensity exercise training alters the effect of N-acetylcysteine on exercise-related muscle ionic shifts in men",
abstract = "This study investigated whether high-intensity exercise training alters the effect of N-acetylcysteine (a precursor of antioxidant glutathione) on exercise-related muscle ionic shifts. We assigned 20 recreationally-active men to 6 weeks of high-intensity exercise training, comprising three weekly sessions of 4–10 × 20-s all-out bouts interspersed by 2 min recovery (SET, n = 10), or habitual lifestyle maintenance (n = 10). Before and after SET, we measured ionic shifts across the working muscle, using leg arteriovenous balance technique, during one-legged knee-extensor exercise to exhaustion with and without N-acetylcysteine infusion. Furthermore, we sampled vastus lateralis muscle biopsies for analyses of metabolites, mitochondrial respiratory function, and proteins regulating ion transport and antioxidant defense. SET lowered exercise-related H+, K+, lactate−, and Na+ shifts and enhanced exercise performance by ≈45%. While N-acetylcysteine did not affect exercise-related ionic shifts before SET, it lowered H+, HCO3−, and Na+ shifts after SET. SET enhanced muscle mitochondrial respiratory capacity and augmented the abundance of Na+/K+-ATPase subunits (α1 and β1), ATP-sensitive K+ channel subunit (Kir6.2), and monocarboxylate transporter-1, as well as superoxide dismutase-2 and glutathione peroxidase-1. Collectively, these findings demonstrate that high-intensity exercise training not only induces multiple adaptations that enhance the ability to counter exercise-related ionic shifts but also potentiates the effect of N-acetylcysteine on ionic shifts during exercise.",
keywords = "Faculty of Science, ROS, Oxygen species, Scavengers, NAC, High-intensity training, Potassium, Lactate, pH, Antioxidant, Performance",
author = "Lemminger, {Anders Krogh} and Matteo Fiorenza and Kasper Eibye and Jens Bangsbo and Morten Hostrup",
note = "CURIS 2023 NEXS 008",
year = "2023",
doi = "10.3390/antiox12010053",
language = "English",
volume = "12",
journal = "Antioxidants",
issn = "2076-3921",
publisher = "M D P I AG",
number = "1",

}

RIS

TY - JOUR

T1 - High-intensity exercise training alters the effect of N-acetylcysteine on exercise-related muscle ionic shifts in men

AU - Lemminger, Anders Krogh

AU - Fiorenza, Matteo

AU - Eibye, Kasper

AU - Bangsbo, Jens

AU - Hostrup, Morten

N1 - CURIS 2023 NEXS 008

PY - 2023

Y1 - 2023

N2 - This study investigated whether high-intensity exercise training alters the effect of N-acetylcysteine (a precursor of antioxidant glutathione) on exercise-related muscle ionic shifts. We assigned 20 recreationally-active men to 6 weeks of high-intensity exercise training, comprising three weekly sessions of 4–10 × 20-s all-out bouts interspersed by 2 min recovery (SET, n = 10), or habitual lifestyle maintenance (n = 10). Before and after SET, we measured ionic shifts across the working muscle, using leg arteriovenous balance technique, during one-legged knee-extensor exercise to exhaustion with and without N-acetylcysteine infusion. Furthermore, we sampled vastus lateralis muscle biopsies for analyses of metabolites, mitochondrial respiratory function, and proteins regulating ion transport and antioxidant defense. SET lowered exercise-related H+, K+, lactate−, and Na+ shifts and enhanced exercise performance by ≈45%. While N-acetylcysteine did not affect exercise-related ionic shifts before SET, it lowered H+, HCO3−, and Na+ shifts after SET. SET enhanced muscle mitochondrial respiratory capacity and augmented the abundance of Na+/K+-ATPase subunits (α1 and β1), ATP-sensitive K+ channel subunit (Kir6.2), and monocarboxylate transporter-1, as well as superoxide dismutase-2 and glutathione peroxidase-1. Collectively, these findings demonstrate that high-intensity exercise training not only induces multiple adaptations that enhance the ability to counter exercise-related ionic shifts but also potentiates the effect of N-acetylcysteine on ionic shifts during exercise.

AB - This study investigated whether high-intensity exercise training alters the effect of N-acetylcysteine (a precursor of antioxidant glutathione) on exercise-related muscle ionic shifts. We assigned 20 recreationally-active men to 6 weeks of high-intensity exercise training, comprising three weekly sessions of 4–10 × 20-s all-out bouts interspersed by 2 min recovery (SET, n = 10), or habitual lifestyle maintenance (n = 10). Before and after SET, we measured ionic shifts across the working muscle, using leg arteriovenous balance technique, during one-legged knee-extensor exercise to exhaustion with and without N-acetylcysteine infusion. Furthermore, we sampled vastus lateralis muscle biopsies for analyses of metabolites, mitochondrial respiratory function, and proteins regulating ion transport and antioxidant defense. SET lowered exercise-related H+, K+, lactate−, and Na+ shifts and enhanced exercise performance by ≈45%. While N-acetylcysteine did not affect exercise-related ionic shifts before SET, it lowered H+, HCO3−, and Na+ shifts after SET. SET enhanced muscle mitochondrial respiratory capacity and augmented the abundance of Na+/K+-ATPase subunits (α1 and β1), ATP-sensitive K+ channel subunit (Kir6.2), and monocarboxylate transporter-1, as well as superoxide dismutase-2 and glutathione peroxidase-1. Collectively, these findings demonstrate that high-intensity exercise training not only induces multiple adaptations that enhance the ability to counter exercise-related ionic shifts but also potentiates the effect of N-acetylcysteine on ionic shifts during exercise.

KW - Faculty of Science

KW - ROS

KW - Oxygen species

KW - Scavengers

KW - NAC

KW - High-intensity training

KW - Potassium

KW - Lactate

KW - pH

KW - Antioxidant

KW - Performance

U2 - 10.3390/antiox12010053

DO - 10.3390/antiox12010053

M3 - Journal article

C2 - 36670915

VL - 12

JO - Antioxidants

JF - Antioxidants

SN - 2076-3921

IS - 1

M1 - 53

ER -

ID: 330895603