Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men

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Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men. / Vigelsø Hansen, Andreas; Prats , C; Ploug, T; Dela, F; Helge, J W.

I: Physiological Research, Bind 65, Nr. 2, 2016, s. 293-302.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Vigelsø Hansen, A, Prats , C, Ploug, T, Dela, F & Helge, JW 2016, 'Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men', Physiological Research, bind 65, nr. 2, s. 293-302. <http://www.biomed.cas.cz/physiolres/pdf/65/65_293.pdf>

APA

Vigelsø Hansen, A., Prats , C., Ploug, T., Dela, F., & Helge, J. W. (2016). Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men. Physiological Research, 65(2), 293-302. http://www.biomed.cas.cz/physiolres/pdf/65/65_293.pdf

Vancouver

Vigelsø Hansen A, Prats C, Ploug T, Dela F, Helge JW. Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men. Physiological Research. 2016;65(2):293-302.

Author

Vigelsø Hansen, Andreas ; Prats , C ; Ploug, T ; Dela, F ; Helge, J W. / Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men. I: Physiological Research. 2016 ; Bind 65, Nr. 2. s. 293-302.

Bibtex

@article{1b29ac6396e1485986c30ed5cc54d744,
title = "Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men",
abstract = "A high VO(2)max in middle-age is related to high metabolic flexibility and lowered risk of metabolic diseases. However, the influence of a high VO(2)max induced by years of regular training in middle-age on protein expression related to muscle metabolism is not well studied. This study measures key proteins involved in mitochondrial oxidation, glucose and lipid metabolism in skeletal muscle of trained and untrained middle-aged men. 16 middle-aged men, matched for lean body mass, were recruited into an endurance trained (TR, n=8) or an untrained (CON, n=8) group based on their VO(2)max. A muscle biopsy was obtained from m. vastus lateralis and protein levels were analysed by Western blotting. The TR had higher protein levels of mitochondrial complex III-V, endothelial lipase (EL) and perilipin 5 compared to the CON. Glycogen synthase (P=0.05), Perilipin 3 (P=0.09) and ATGL (P=0.09) tended to be higher in TR than CON, but there was no difference in AKT I/II, HKII, GLUT4 and LPL protein expression. Lastly, there was a positive correlation between plasma HDL and EL (R(2)=0.53, P<0.01). In conclusion, a high VO(2)max in middle-aged men was as expected is reflected in higher muscle oxidative capacity, but also in higher endothelial lipase and perilipin 5 expression and a borderline higher glycogen synthase protein expression, which may contribute to a higher metabolic flexibility.",
author = "{Vigels{\o} Hansen}, Andreas and C Prats and T Ploug and F Dela and Helge, {J W}",
year = "2016",
language = "English",
volume = "65",
pages = "293--302",
journal = "Physiological Research",
issn = "0862-8408",
publisher = "Akademie Ved Ceske Republiky Fyziologicky Ustav",
number = "2",

}

RIS

TY - JOUR

T1 - Higher muscle content of perilipin 5 and endothelial lipase protein in trained than untrained middle-aged men

AU - Vigelsø Hansen, Andreas

AU - Prats , C

AU - Ploug, T

AU - Dela, F

AU - Helge, J W

PY - 2016

Y1 - 2016

N2 - A high VO(2)max in middle-age is related to high metabolic flexibility and lowered risk of metabolic diseases. However, the influence of a high VO(2)max induced by years of regular training in middle-age on protein expression related to muscle metabolism is not well studied. This study measures key proteins involved in mitochondrial oxidation, glucose and lipid metabolism in skeletal muscle of trained and untrained middle-aged men. 16 middle-aged men, matched for lean body mass, were recruited into an endurance trained (TR, n=8) or an untrained (CON, n=8) group based on their VO(2)max. A muscle biopsy was obtained from m. vastus lateralis and protein levels were analysed by Western blotting. The TR had higher protein levels of mitochondrial complex III-V, endothelial lipase (EL) and perilipin 5 compared to the CON. Glycogen synthase (P=0.05), Perilipin 3 (P=0.09) and ATGL (P=0.09) tended to be higher in TR than CON, but there was no difference in AKT I/II, HKII, GLUT4 and LPL protein expression. Lastly, there was a positive correlation between plasma HDL and EL (R(2)=0.53, P<0.01). In conclusion, a high VO(2)max in middle-aged men was as expected is reflected in higher muscle oxidative capacity, but also in higher endothelial lipase and perilipin 5 expression and a borderline higher glycogen synthase protein expression, which may contribute to a higher metabolic flexibility.

AB - A high VO(2)max in middle-age is related to high metabolic flexibility and lowered risk of metabolic diseases. However, the influence of a high VO(2)max induced by years of regular training in middle-age on protein expression related to muscle metabolism is not well studied. This study measures key proteins involved in mitochondrial oxidation, glucose and lipid metabolism in skeletal muscle of trained and untrained middle-aged men. 16 middle-aged men, matched for lean body mass, were recruited into an endurance trained (TR, n=8) or an untrained (CON, n=8) group based on their VO(2)max. A muscle biopsy was obtained from m. vastus lateralis and protein levels were analysed by Western blotting. The TR had higher protein levels of mitochondrial complex III-V, endothelial lipase (EL) and perilipin 5 compared to the CON. Glycogen synthase (P=0.05), Perilipin 3 (P=0.09) and ATGL (P=0.09) tended to be higher in TR than CON, but there was no difference in AKT I/II, HKII, GLUT4 and LPL protein expression. Lastly, there was a positive correlation between plasma HDL and EL (R(2)=0.53, P<0.01). In conclusion, a high VO(2)max in middle-aged men was as expected is reflected in higher muscle oxidative capacity, but also in higher endothelial lipase and perilipin 5 expression and a borderline higher glycogen synthase protein expression, which may contribute to a higher metabolic flexibility.

M3 - Journal article

C2 - 26447519

VL - 65

SP - 293

EP - 302

JO - Physiological Research

JF - Physiological Research

SN - 0862-8408

IS - 2

ER -

ID: 146737625