GLP-1 Amidation Efficiency Along the Length of the Intestine in Mice, Rats and Pigs and in GLP-1 Secreting Cell Lines

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GLP-1 Amidation Efficiency Along the Length of the Intestine in Mice, Rats and Pigs and in GLP-1 Secreting Cell Lines. / Kuhre, Rune Ehrenreich; Albrechtsen, Nicolai Jacob Wewer; Windeløv, Johanne Agerlin; Svendsen, Berit; Hartmann, Bolette; Holst, Jens Juul.

I: Peptides, Bind 55, 31.01.2014, s. 52-57.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Kuhre, RE, Albrechtsen, NJW, Windeløv, JA, Svendsen, B, Hartmann, B & Holst, JJ 2014, 'GLP-1 Amidation Efficiency Along the Length of the Intestine in Mice, Rats and Pigs and in GLP-1 Secreting Cell Lines', Peptides, bind 55, s. 52-57. https://doi.org/10.1016/j.peptides.2014.01.020

APA

Kuhre, R. E., Albrechtsen, N. J. W., Windeløv, J. A., Svendsen, B., Hartmann, B., & Holst, J. J. (2014). GLP-1 Amidation Efficiency Along the Length of the Intestine in Mice, Rats and Pigs and in GLP-1 Secreting Cell Lines. Peptides, 55, 52-57. https://doi.org/10.1016/j.peptides.2014.01.020

Vancouver

Kuhre RE, Albrechtsen NJW, Windeløv JA, Svendsen B, Hartmann B, Holst JJ. GLP-1 Amidation Efficiency Along the Length of the Intestine in Mice, Rats and Pigs and in GLP-1 Secreting Cell Lines. Peptides. 2014 jan 31;55:52-57. https://doi.org/10.1016/j.peptides.2014.01.020

Author

Kuhre, Rune Ehrenreich ; Albrechtsen, Nicolai Jacob Wewer ; Windeløv, Johanne Agerlin ; Svendsen, Berit ; Hartmann, Bolette ; Holst, Jens Juul. / GLP-1 Amidation Efficiency Along the Length of the Intestine in Mice, Rats and Pigs and in GLP-1 Secreting Cell Lines. I: Peptides. 2014 ; Bind 55. s. 52-57.

Bibtex

@article{1fb9f299dc9e4eafa0a458d5ce183c35,
title = "GLP-1 Amidation Efficiency Along the Length of the Intestine in Mice, Rats and Pigs and in GLP-1 Secreting Cell Lines",
abstract = "XXX: Measurements of plasma concentrations of the incretin hormone GLP-1 are complex because of extensive molecular heterogeneity. This is partly due to a varying and incompletely known degree of C-terminal amidation. Given that virtually all GLP-1 assays rely on a C-terminal antibody, it is essential to know whether or not the molecule one wants to measure is amidated. We performed a detailed analysis of extractable GLP-1 from duodenum, proximal jejunum, distal ileum, caecum, proximal colon and distal colon of mice (n=9), rats (n=9) and pigs (n=8) and determined the degree of amidation and whether this varied with the six different locations. We also analyzed the amidation in 3 GLP-1 secreting cell lines (GLUTag, NCI-H716 and STC-1). To our surprise there were marked differences between the 3 species with respect to the concentration of GLP-1 in gut. In the mouse, concentrations increased continuously along the intestine all the way to the rectum, but were highest in the distal ileum and proximal colon of the rat. In the pig, very little or no GLP-1 was present before the distal ileum with similar levels from ileum to distal colon. In the mouse, GLP-1 was extensively amidated at all sampling sites, whereas rats and pigs on average had around 2.5 and 4 times higher levels of amidated compared to non-amidated GLP-1, although the ratio varied depending upon the location. GLUTag, NCI-H716 and STC-1 cells all exhibited partial amidation with 2-4 times higher levels of amidated compared to non-amidated GLP-1.",
keywords = "Faculty of Health and Medical Sciences, Glucagon-Like Peptide 1, Amidation, Mice, Rats, Pigs and GLP-1 secreting cell lines",
author = "Kuhre, {Rune Ehrenreich} and Albrechtsen, {Nicolai Jacob Wewer} and Windel{\o}v, {Johanne Agerlin} and Berit Svendsen and Bolette Hartmann and Holst, {Jens Juul}",
note = "Copyright {\circledC} 2014. Published by Elsevier Inc.",
year = "2014",
month = "1",
day = "31",
doi = "10.1016/j.peptides.2014.01.020",
language = "English",
volume = "55",
pages = "52--57",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - GLP-1 Amidation Efficiency Along the Length of the Intestine in Mice, Rats and Pigs and in GLP-1 Secreting Cell Lines

AU - Kuhre, Rune Ehrenreich

AU - Albrechtsen, Nicolai Jacob Wewer

AU - Windeløv, Johanne Agerlin

AU - Svendsen, Berit

AU - Hartmann, Bolette

AU - Holst, Jens Juul

N1 - Copyright © 2014. Published by Elsevier Inc.

PY - 2014/1/31

Y1 - 2014/1/31

N2 - XXX: Measurements of plasma concentrations of the incretin hormone GLP-1 are complex because of extensive molecular heterogeneity. This is partly due to a varying and incompletely known degree of C-terminal amidation. Given that virtually all GLP-1 assays rely on a C-terminal antibody, it is essential to know whether or not the molecule one wants to measure is amidated. We performed a detailed analysis of extractable GLP-1 from duodenum, proximal jejunum, distal ileum, caecum, proximal colon and distal colon of mice (n=9), rats (n=9) and pigs (n=8) and determined the degree of amidation and whether this varied with the six different locations. We also analyzed the amidation in 3 GLP-1 secreting cell lines (GLUTag, NCI-H716 and STC-1). To our surprise there were marked differences between the 3 species with respect to the concentration of GLP-1 in gut. In the mouse, concentrations increased continuously along the intestine all the way to the rectum, but were highest in the distal ileum and proximal colon of the rat. In the pig, very little or no GLP-1 was present before the distal ileum with similar levels from ileum to distal colon. In the mouse, GLP-1 was extensively amidated at all sampling sites, whereas rats and pigs on average had around 2.5 and 4 times higher levels of amidated compared to non-amidated GLP-1, although the ratio varied depending upon the location. GLUTag, NCI-H716 and STC-1 cells all exhibited partial amidation with 2-4 times higher levels of amidated compared to non-amidated GLP-1.

AB - XXX: Measurements of plasma concentrations of the incretin hormone GLP-1 are complex because of extensive molecular heterogeneity. This is partly due to a varying and incompletely known degree of C-terminal amidation. Given that virtually all GLP-1 assays rely on a C-terminal antibody, it is essential to know whether or not the molecule one wants to measure is amidated. We performed a detailed analysis of extractable GLP-1 from duodenum, proximal jejunum, distal ileum, caecum, proximal colon and distal colon of mice (n=9), rats (n=9) and pigs (n=8) and determined the degree of amidation and whether this varied with the six different locations. We also analyzed the amidation in 3 GLP-1 secreting cell lines (GLUTag, NCI-H716 and STC-1). To our surprise there were marked differences between the 3 species with respect to the concentration of GLP-1 in gut. In the mouse, concentrations increased continuously along the intestine all the way to the rectum, but were highest in the distal ileum and proximal colon of the rat. In the pig, very little or no GLP-1 was present before the distal ileum with similar levels from ileum to distal colon. In the mouse, GLP-1 was extensively amidated at all sampling sites, whereas rats and pigs on average had around 2.5 and 4 times higher levels of amidated compared to non-amidated GLP-1, although the ratio varied depending upon the location. GLUTag, NCI-H716 and STC-1 cells all exhibited partial amidation with 2-4 times higher levels of amidated compared to non-amidated GLP-1.

KW - Faculty of Health and Medical Sciences

KW - Glucagon-Like Peptide 1

KW - Amidation

KW - Mice, Rats, Pigs and GLP-1 secreting cell lines

U2 - 10.1016/j.peptides.2014.01.020

DO - 10.1016/j.peptides.2014.01.020

M3 - Journal article

C2 - 24486427

VL - 55

SP - 52

EP - 57

JO - Peptides

JF - Peptides

SN - 0196-9781

ER -

ID: 98831040