Gene probes to detect cross-culture contamination in hormone producing cell lines

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Standard

Gene probes to detect cross-culture contamination in hormone producing cell lines. / Matsuba, I; Lernmark, A; Madsen, Ole Dragsbæk; Michelsen, Birgitte; Nielsen, Jens Høiriis; Scholler, John; Vissing, H; Welinder, B; Tommerup, N; Mikkelsen, M.

I: In Vitro Cellular & Developmental Biology, Bind 24, Nr. 11, 11.1988, s. 1071-6.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Matsuba, I, Lernmark, A, Madsen, OD, Michelsen, B, Nielsen, JH, Scholler, J, Vissing, H, Welinder, B, Tommerup, N & Mikkelsen, M 1988, 'Gene probes to detect cross-culture contamination in hormone producing cell lines', In Vitro Cellular & Developmental Biology, bind 24, nr. 11, s. 1071-6. https://doi.org/10.1007/BF02620807

APA

Matsuba, I., Lernmark, A., Madsen, O. D., Michelsen, B., Nielsen, J. H., Scholler, J., Vissing, H., Welinder, B., Tommerup, N., & Mikkelsen, M. (1988). Gene probes to detect cross-culture contamination in hormone producing cell lines. In Vitro Cellular & Developmental Biology, 24(11), 1071-6. https://doi.org/10.1007/BF02620807

Vancouver

Matsuba I, Lernmark A, Madsen OD, Michelsen B, Nielsen JH, Scholler J o.a. Gene probes to detect cross-culture contamination in hormone producing cell lines. In Vitro Cellular & Developmental Biology. 1988 nov.;24(11):1071-6. https://doi.org/10.1007/BF02620807

Author

Matsuba, I ; Lernmark, A ; Madsen, Ole Dragsbæk ; Michelsen, Birgitte ; Nielsen, Jens Høiriis ; Scholler, John ; Vissing, H ; Welinder, B ; Tommerup, N ; Mikkelsen, M. / Gene probes to detect cross-culture contamination in hormone producing cell lines. I: In Vitro Cellular & Developmental Biology. 1988 ; Bind 24, Nr. 11. s. 1071-6.

Bibtex

@article{2ccd77190c25452681e7b8a2675d0b56,
title = "Gene probes to detect cross-culture contamination in hormone producing cell lines",
abstract = "Cross-culture contamination of cell lines propagated in continuous culture is a frequent event and particularly difficult to resolve in cells expressing similar phenotypes. We demonstrate that DNA-DNA hybridization to blotted endonuclease-digested cell DNA effectively detects cross-culture contamination to monitor inter-species as well as intra-species cross contamination. An insulin-producing cell-line, Clone-16, originally cloned from a human fetal endocrine pancreatic cell line did not produce human c-peptide as anticipated. DNA from these cells showed no hybridization to the human ALU sequence probe, BLUR, and lacked restriction fragment length polymorphism typical for the human HLA-DQ beta-chain gene. Although a human insulin gene probe showed a weak, nonhuman hybridization pattern, a cDNA probe for the Syrian hamster insulin gene hybridized strongly consistent with a single copy hamster insulin gene. Karyotyping confirmed the absence of human chromosomes in the Clone-16 cells while sizes, centromere indices, and banding patterns were identical to Syrian hamster fibroblasts. We conclude that the insulin-producing Clone-16 cells are of Syrian hamster origin and demonstrate the effective use of gene probes to control the origin of cell cultures.",
keywords = "Adenoma, Islet Cell, Animals, Blotting, Southern, Cell Line, Chromosome Banding, Cricetinae, DNA Probes, HLA-DQ Antigens, Humans, Insulin, Insulinoma, Mesocricetus, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length, Repetitive Sequences, Nucleic Acid",
author = "I Matsuba and A Lernmark and Madsen, {Ole Dragsb{\ae}k} and Birgitte Michelsen and Nielsen, {Jens H{\o}iriis} and John Scholler and H Vissing and B Welinder and N Tommerup and M Mikkelsen",
year = "1988",
month = nov,
doi = "10.1007/BF02620807",
language = "English",
volume = "24",
pages = "1071--6",
journal = "In Vitro Cellular & Developmental Biology",
issn = "0883-8364",
publisher = "Society for In Vitro Biology",
number = "11",

}

RIS

TY - JOUR

T1 - Gene probes to detect cross-culture contamination in hormone producing cell lines

AU - Matsuba, I

AU - Lernmark, A

AU - Madsen, Ole Dragsbæk

AU - Michelsen, Birgitte

AU - Nielsen, Jens Høiriis

AU - Scholler, John

AU - Vissing, H

AU - Welinder, B

AU - Tommerup, N

AU - Mikkelsen, M

PY - 1988/11

Y1 - 1988/11

N2 - Cross-culture contamination of cell lines propagated in continuous culture is a frequent event and particularly difficult to resolve in cells expressing similar phenotypes. We demonstrate that DNA-DNA hybridization to blotted endonuclease-digested cell DNA effectively detects cross-culture contamination to monitor inter-species as well as intra-species cross contamination. An insulin-producing cell-line, Clone-16, originally cloned from a human fetal endocrine pancreatic cell line did not produce human c-peptide as anticipated. DNA from these cells showed no hybridization to the human ALU sequence probe, BLUR, and lacked restriction fragment length polymorphism typical for the human HLA-DQ beta-chain gene. Although a human insulin gene probe showed a weak, nonhuman hybridization pattern, a cDNA probe for the Syrian hamster insulin gene hybridized strongly consistent with a single copy hamster insulin gene. Karyotyping confirmed the absence of human chromosomes in the Clone-16 cells while sizes, centromere indices, and banding patterns were identical to Syrian hamster fibroblasts. We conclude that the insulin-producing Clone-16 cells are of Syrian hamster origin and demonstrate the effective use of gene probes to control the origin of cell cultures.

AB - Cross-culture contamination of cell lines propagated in continuous culture is a frequent event and particularly difficult to resolve in cells expressing similar phenotypes. We demonstrate that DNA-DNA hybridization to blotted endonuclease-digested cell DNA effectively detects cross-culture contamination to monitor inter-species as well as intra-species cross contamination. An insulin-producing cell-line, Clone-16, originally cloned from a human fetal endocrine pancreatic cell line did not produce human c-peptide as anticipated. DNA from these cells showed no hybridization to the human ALU sequence probe, BLUR, and lacked restriction fragment length polymorphism typical for the human HLA-DQ beta-chain gene. Although a human insulin gene probe showed a weak, nonhuman hybridization pattern, a cDNA probe for the Syrian hamster insulin gene hybridized strongly consistent with a single copy hamster insulin gene. Karyotyping confirmed the absence of human chromosomes in the Clone-16 cells while sizes, centromere indices, and banding patterns were identical to Syrian hamster fibroblasts. We conclude that the insulin-producing Clone-16 cells are of Syrian hamster origin and demonstrate the effective use of gene probes to control the origin of cell cultures.

KW - Adenoma, Islet Cell

KW - Animals

KW - Blotting, Southern

KW - Cell Line

KW - Chromosome Banding

KW - Cricetinae

KW - DNA Probes

KW - HLA-DQ Antigens

KW - Humans

KW - Insulin

KW - Insulinoma

KW - Mesocricetus

KW - Polymorphism, Genetic

KW - Polymorphism, Restriction Fragment Length

KW - Repetitive Sequences, Nucleic Acid

U2 - 10.1007/BF02620807

DO - 10.1007/BF02620807

M3 - Journal article

C2 - 2903855

VL - 24

SP - 1071

EP - 1076

JO - In Vitro Cellular & Developmental Biology

JF - In Vitro Cellular & Developmental Biology

SN - 0883-8364

IS - 11

ER -

ID: 47974645