Expression and Localization of microRNAs in Perinatal Rat Pancreas: Role of miR-21 in Regulation of Cholesterol Metabolism

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Standard

Expression and Localization of microRNAs in Perinatal Rat Pancreas : Role of miR-21 in Regulation of Cholesterol Metabolism. / Larsen, Louise; Rosenstierne, Maiken Worsøe; Gaarn, Louise Winkel; Bagge, Annika; Pedersen, Lykke; Dahmcke, Christina M.; Nielsen, Jens Høiriis; Dalgaard, Louise T.

I: PLOS ONE, Bind 6, Nr. 10, 11.10.2011, s. e25997.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Larsen, L, Rosenstierne, MW, Gaarn, LW, Bagge, A, Pedersen, L, Dahmcke, CM, Nielsen, JH & Dalgaard, LT 2011, 'Expression and Localization of microRNAs in Perinatal Rat Pancreas: Role of miR-21 in Regulation of Cholesterol Metabolism', PLOS ONE, bind 6, nr. 10, s. e25997. https://doi.org/10.1371/journal.pone.0025997

APA

Larsen, L., Rosenstierne, M. W., Gaarn, L. W., Bagge, A., Pedersen, L., Dahmcke, C. M., ... Dalgaard, L. T. (2011). Expression and Localization of microRNAs in Perinatal Rat Pancreas: Role of miR-21 in Regulation of Cholesterol Metabolism. PLOS ONE, 6(10), e25997. https://doi.org/10.1371/journal.pone.0025997

Vancouver

Larsen L, Rosenstierne MW, Gaarn LW, Bagge A, Pedersen L, Dahmcke CM o.a. Expression and Localization of microRNAs in Perinatal Rat Pancreas: Role of miR-21 in Regulation of Cholesterol Metabolism. PLOS ONE. 2011 okt 11;6(10):e25997. https://doi.org/10.1371/journal.pone.0025997

Author

Larsen, Louise ; Rosenstierne, Maiken Worsøe ; Gaarn, Louise Winkel ; Bagge, Annika ; Pedersen, Lykke ; Dahmcke, Christina M. ; Nielsen, Jens Høiriis ; Dalgaard, Louise T. / Expression and Localization of microRNAs in Perinatal Rat Pancreas : Role of miR-21 in Regulation of Cholesterol Metabolism. I: PLOS ONE. 2011 ; Bind 6, Nr. 10. s. e25997.

Bibtex

@article{e2d931f721d84f72a4394f7d563bbfd9,
title = "Expression and Localization of microRNAs in Perinatal Rat Pancreas: Role of miR-21 in Regulation of Cholesterol Metabolism",
abstract = "OBJECTIVE:To investigate the expression of pancreatic microRNAs (miRNAs) during the period of perinatal beta-cell expansion and maturation in rats, determine the localization of these miRNAs and perform a pathway analysis with predicted target mRNAs expressed in perinatal pancreas.RESEARCH DESIGN AND METHODS:RNA was extracted from whole pancreas at embryonic day 20 (E20), on the day of birth (P0) and two days after birth (P2) and hybridized to miRNA microarrays. Differentially expressed miRNAs were verified by northern blotting and their pancreatic localization determined by in situ hybridization. Pathway analysis was done using regulated sets of mRNAs predicted as targets of the miRNAs. Possible target genes were tested using reporter-gene analysis in INS-1E cells.RESULTS:Nine miRNAs were differentially expressed perinatally, seven were confirmed to be regulated at the level of the mature miRNA. The localization studies showed endocrine localization of six of these miRNAs (miR-21, -23a, -29a, -125b-5p, -376b-3p and -451), and all were expressed in exocrine cells at one time point at least. Pathways involving metabolic processes, terpenoid and sterol metabolism were selectively affected by concomitant regulation by miRNAs and mRNAs, and Srebf1 was validated as a target of miR-21.CONCLUSIONS:The findings suggest that miRNAs are involved in the functional maturation of pancreatic exocrine and endocrine tissue following birth. Pathway analysis of target genes identify changes in sterol metabolism around birth as being selectively affected by differential miRNA expression during this period.",
author = "Louise Larsen and Rosenstierne, {Maiken Wors{\o}e} and Gaarn, {Louise Winkel} and Annika Bagge and Lykke Pedersen and Dahmcke, {Christina M.} and Nielsen, {Jens H{\o}iriis} and Dalgaard, {Louise T.}",
year = "2011",
month = "10",
day = "11",
doi = "10.1371/journal.pone.0025997",
language = "English",
volume = "6",
pages = "e25997",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",

}

RIS

TY - JOUR

T1 - Expression and Localization of microRNAs in Perinatal Rat Pancreas

T2 - Role of miR-21 in Regulation of Cholesterol Metabolism

AU - Larsen, Louise

AU - Rosenstierne, Maiken Worsøe

AU - Gaarn, Louise Winkel

AU - Bagge, Annika

AU - Pedersen, Lykke

AU - Dahmcke, Christina M.

AU - Nielsen, Jens Høiriis

AU - Dalgaard, Louise T.

PY - 2011/10/11

Y1 - 2011/10/11

N2 - OBJECTIVE:To investigate the expression of pancreatic microRNAs (miRNAs) during the period of perinatal beta-cell expansion and maturation in rats, determine the localization of these miRNAs and perform a pathway analysis with predicted target mRNAs expressed in perinatal pancreas.RESEARCH DESIGN AND METHODS:RNA was extracted from whole pancreas at embryonic day 20 (E20), on the day of birth (P0) and two days after birth (P2) and hybridized to miRNA microarrays. Differentially expressed miRNAs were verified by northern blotting and their pancreatic localization determined by in situ hybridization. Pathway analysis was done using regulated sets of mRNAs predicted as targets of the miRNAs. Possible target genes were tested using reporter-gene analysis in INS-1E cells.RESULTS:Nine miRNAs were differentially expressed perinatally, seven were confirmed to be regulated at the level of the mature miRNA. The localization studies showed endocrine localization of six of these miRNAs (miR-21, -23a, -29a, -125b-5p, -376b-3p and -451), and all were expressed in exocrine cells at one time point at least. Pathways involving metabolic processes, terpenoid and sterol metabolism were selectively affected by concomitant regulation by miRNAs and mRNAs, and Srebf1 was validated as a target of miR-21.CONCLUSIONS:The findings suggest that miRNAs are involved in the functional maturation of pancreatic exocrine and endocrine tissue following birth. Pathway analysis of target genes identify changes in sterol metabolism around birth as being selectively affected by differential miRNA expression during this period.

AB - OBJECTIVE:To investigate the expression of pancreatic microRNAs (miRNAs) during the period of perinatal beta-cell expansion and maturation in rats, determine the localization of these miRNAs and perform a pathway analysis with predicted target mRNAs expressed in perinatal pancreas.RESEARCH DESIGN AND METHODS:RNA was extracted from whole pancreas at embryonic day 20 (E20), on the day of birth (P0) and two days after birth (P2) and hybridized to miRNA microarrays. Differentially expressed miRNAs were verified by northern blotting and their pancreatic localization determined by in situ hybridization. Pathway analysis was done using regulated sets of mRNAs predicted as targets of the miRNAs. Possible target genes were tested using reporter-gene analysis in INS-1E cells.RESULTS:Nine miRNAs were differentially expressed perinatally, seven were confirmed to be regulated at the level of the mature miRNA. The localization studies showed endocrine localization of six of these miRNAs (miR-21, -23a, -29a, -125b-5p, -376b-3p and -451), and all were expressed in exocrine cells at one time point at least. Pathways involving metabolic processes, terpenoid and sterol metabolism were selectively affected by concomitant regulation by miRNAs and mRNAs, and Srebf1 was validated as a target of miR-21.CONCLUSIONS:The findings suggest that miRNAs are involved in the functional maturation of pancreatic exocrine and endocrine tissue following birth. Pathway analysis of target genes identify changes in sterol metabolism around birth as being selectively affected by differential miRNA expression during this period.

U2 - 10.1371/journal.pone.0025997

DO - 10.1371/journal.pone.0025997

M3 - Journal article

VL - 6

SP - e25997

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 10

ER -

ID: 35040789