Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins

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Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins. / Ponce-González, Jesús Gustavo; Ara, Ignacio; Larsen, Steen; Guerra, Borja; Calbet, Jose A L; Helge, Jørn Wulff.

I: European Journal of Applied Physiology, Bind 116, Nr. 1, 01.2016.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Ponce-González, JG, Ara, I, Larsen, S, Guerra, B, Calbet, JAL & Helge, JW 2016, 'Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins', European Journal of Applied Physiology, bind 116, nr. 1. https://doi.org/10.1007/s00421-015-3232-7

APA

Ponce-González, J. G., Ara, I., Larsen, S., Guerra, B., Calbet, J. A. L., & Helge, J. W. (2016). Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins. European Journal of Applied Physiology, 116(1). https://doi.org/10.1007/s00421-015-3232-7

Vancouver

Ponce-González JG, Ara I, Larsen S, Guerra B, Calbet JAL, Helge JW. Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins. European Journal of Applied Physiology. 2016 jan;116(1). https://doi.org/10.1007/s00421-015-3232-7

Author

Ponce-González, Jesús Gustavo ; Ara, Ignacio ; Larsen, Steen ; Guerra, Borja ; Calbet, Jose A L ; Helge, Jørn Wulff. / Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins. I: European Journal of Applied Physiology. 2016 ; Bind 116, Nr. 1.

Bibtex

@article{9921c501aadc4fa495ee0bcef7262f39,
title = "Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins",
abstract = "PURPOSE: The aim of this study was to determine if the expression of the mitochondrial biogenesis-regulating proteins SIRT1, SIRT3 and PGC-1alpha in human skeletal muscle is influenced by adiposity.METHOD: Twenty-nine male subjects were recruited into three groups: control (n = 10), obese (n = 10) and post-obese (n = 9). Intentionally, groups were matched by age, aerobic capacity and in addition the control and post-obese groups also by BMI. Muscle biopsies were obtained from the m. deltoid and vastus lateralis. PGC-1alpha, SIRT1 and SIRT3 protein expression was analyzed by Western blot.RESULT: PGC-1alpha, SIRT1 and SIRT3 protein expression was similar regardless of the level of adiposity. Only a main effect of group on SIRT1 protein showed a trend toward higher expression in post-obese than control and obese (P = 0.09). Despite similar muscle fiber-type composition (previously reported), PGC-1alpha, SIRT1 and SIRT3 protein expression was higher in leg compared to arm muscle in all groups (P < 0.05).CONCLUSION: This study shows that PGC-1alpha, SIRT1 and SIRT3 protein expression in basal conditions was not altered in humans with different levels of adiposity but similar aerobic capacity. The expression of PGC-1alpha, SIRT1 and SIRT3 was higher in vastus lateralis than in deltoid muscle, indicating that local rather than systemic factors prevail in regulating the level of expression of these proteins.",
author = "Ponce-Gonz{\'a}lez, {Jes{\'u}s Gustavo} and Ignacio Ara and Steen Larsen and Borja Guerra and Calbet, {Jose A L} and Helge, {J{\o}rn Wulff}",
year = "2016",
month = "1",
doi = "10.1007/s00421-015-3232-7",
language = "English",
volume = "116",
journal = "European Journal of Applied Physiology",
issn = "1439-6319",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Effect of regional muscle location but not adiposity on mitochondrial biogenesis-regulating proteins

AU - Ponce-González, Jesús Gustavo

AU - Ara, Ignacio

AU - Larsen, Steen

AU - Guerra, Borja

AU - Calbet, Jose A L

AU - Helge, Jørn Wulff

PY - 2016/1

Y1 - 2016/1

N2 - PURPOSE: The aim of this study was to determine if the expression of the mitochondrial biogenesis-regulating proteins SIRT1, SIRT3 and PGC-1alpha in human skeletal muscle is influenced by adiposity.METHOD: Twenty-nine male subjects were recruited into three groups: control (n = 10), obese (n = 10) and post-obese (n = 9). Intentionally, groups were matched by age, aerobic capacity and in addition the control and post-obese groups also by BMI. Muscle biopsies were obtained from the m. deltoid and vastus lateralis. PGC-1alpha, SIRT1 and SIRT3 protein expression was analyzed by Western blot.RESULT: PGC-1alpha, SIRT1 and SIRT3 protein expression was similar regardless of the level of adiposity. Only a main effect of group on SIRT1 protein showed a trend toward higher expression in post-obese than control and obese (P = 0.09). Despite similar muscle fiber-type composition (previously reported), PGC-1alpha, SIRT1 and SIRT3 protein expression was higher in leg compared to arm muscle in all groups (P < 0.05).CONCLUSION: This study shows that PGC-1alpha, SIRT1 and SIRT3 protein expression in basal conditions was not altered in humans with different levels of adiposity but similar aerobic capacity. The expression of PGC-1alpha, SIRT1 and SIRT3 was higher in vastus lateralis than in deltoid muscle, indicating that local rather than systemic factors prevail in regulating the level of expression of these proteins.

AB - PURPOSE: The aim of this study was to determine if the expression of the mitochondrial biogenesis-regulating proteins SIRT1, SIRT3 and PGC-1alpha in human skeletal muscle is influenced by adiposity.METHOD: Twenty-nine male subjects were recruited into three groups: control (n = 10), obese (n = 10) and post-obese (n = 9). Intentionally, groups were matched by age, aerobic capacity and in addition the control and post-obese groups also by BMI. Muscle biopsies were obtained from the m. deltoid and vastus lateralis. PGC-1alpha, SIRT1 and SIRT3 protein expression was analyzed by Western blot.RESULT: PGC-1alpha, SIRT1 and SIRT3 protein expression was similar regardless of the level of adiposity. Only a main effect of group on SIRT1 protein showed a trend toward higher expression in post-obese than control and obese (P = 0.09). Despite similar muscle fiber-type composition (previously reported), PGC-1alpha, SIRT1 and SIRT3 protein expression was higher in leg compared to arm muscle in all groups (P < 0.05).CONCLUSION: This study shows that PGC-1alpha, SIRT1 and SIRT3 protein expression in basal conditions was not altered in humans with different levels of adiposity but similar aerobic capacity. The expression of PGC-1alpha, SIRT1 and SIRT3 was higher in vastus lateralis than in deltoid muscle, indicating that local rather than systemic factors prevail in regulating the level of expression of these proteins.

U2 - 10.1007/s00421-015-3232-7

DO - 10.1007/s00421-015-3232-7

M3 - Journal article

VL - 116

JO - European Journal of Applied Physiology

JF - European Journal of Applied Physiology

SN - 1439-6319

IS - 1

ER -

ID: 146737721