Effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets

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Standard

Effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets. / Hansen, Birgit Sehested; Linde, S; Spinas, G A; Welinder, B S; Mandrup-Poulsen, T; Nerup, J; Nielsen, Jens Høiriis.

I: Biomedica Biochimica Acta, Bind 47, Nr. 4-5, 1988, s. 305-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Hansen, BS, Linde, S, Spinas, GA, Welinder, BS, Mandrup-Poulsen, T, Nerup, J & Nielsen, JH 1988, 'Effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets', Biomedica Biochimica Acta, bind 47, nr. 4-5, s. 305-9.

APA

Hansen, B. S., Linde, S., Spinas, G. A., Welinder, B. S., Mandrup-Poulsen, T., Nerup, J., & Nielsen, J. H. (1988). Effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets. Biomedica Biochimica Acta, 47(4-5), 305-9.

Vancouver

Hansen BS, Linde S, Spinas GA, Welinder BS, Mandrup-Poulsen T, Nerup J o.a. Effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets. Biomedica Biochimica Acta. 1988;47(4-5):305-9.

Author

Hansen, Birgit Sehested ; Linde, S ; Spinas, G A ; Welinder, B S ; Mandrup-Poulsen, T ; Nerup, J ; Nielsen, Jens Høiriis. / Effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets. I: Biomedica Biochimica Acta. 1988 ; Bind 47, Nr. 4-5. s. 305-9.

Bibtex

@article{06eca5bdecfc499a96231541003c2e26,
title = "Effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets",
abstract = "Insulin dependent diabetes mellitus (IDDM) is often preceded or associated with lymphocytic infiltration in the islets of Langerhans (insulitis). We recently demonstrated that interleukin-1 (IL-1) produced by activated macrophages exerts a bimodal effect on insulin release and biosynthesis in isolated rat islets. In the present study we have further analysed the effect of recombinant human interleukin-1 beta (rIL-1) on the biosynthesis and conversion of proinsulin 1 and 2 in rat islets. By RP-HPLC-analysis of islets labelled with [3H]leucine we found that exposure to 6 ng/ml of IL-1 for 24 h reduced the insulin biosynthesis to 6.1 +/- 2.7{\%} (n = 4). During the 3 h labelling period the labelled proinsulin content compared to insulin was increased from 9.0 +/- 1.3{\%} (control) to 26.6 +/- 6.4{\%} in the IL-1 exposed islets, and the ratio between labelled insulin 1 to 2 was increased from 2.0 +/- 0.1 to 3.4 +/- 0.4, respectively. Pulse-chase experiments with [3H]leucine and [35S]methionine indicated a more marked reduction in the conversion rate of proinsulin-2 compared to that of proinsulin-1. In conclusion these experiments demonstrate that IL-1 inhibits insulin biosynthesis by preferential attenuation of the rate of conversion of proinsulin 2.",
keywords = "Animals, Cells, Cultured, Humans, Insulin, Interleukin-1, Islets of Langerhans, Kinetics, Proinsulin, Rats, Recombinant Proteins",
author = "Hansen, {Birgit Sehested} and S Linde and Spinas, {G A} and Welinder, {B S} and T Mandrup-Poulsen and J Nerup and Nielsen, {Jens H{\o}iriis}",
year = "1988",
language = "English",
volume = "47",
pages = "305--9",
journal = "Biomedica Biochimica Acta",
issn = "0232-766X",
publisher = "Akademie Verlag GMBH",
number = "4-5",

}

RIS

TY - JOUR

T1 - Effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets

AU - Hansen, Birgit Sehested

AU - Linde, S

AU - Spinas, G A

AU - Welinder, B S

AU - Mandrup-Poulsen, T

AU - Nerup, J

AU - Nielsen, Jens Høiriis

PY - 1988

Y1 - 1988

N2 - Insulin dependent diabetes mellitus (IDDM) is often preceded or associated with lymphocytic infiltration in the islets of Langerhans (insulitis). We recently demonstrated that interleukin-1 (IL-1) produced by activated macrophages exerts a bimodal effect on insulin release and biosynthesis in isolated rat islets. In the present study we have further analysed the effect of recombinant human interleukin-1 beta (rIL-1) on the biosynthesis and conversion of proinsulin 1 and 2 in rat islets. By RP-HPLC-analysis of islets labelled with [3H]leucine we found that exposure to 6 ng/ml of IL-1 for 24 h reduced the insulin biosynthesis to 6.1 +/- 2.7% (n = 4). During the 3 h labelling period the labelled proinsulin content compared to insulin was increased from 9.0 +/- 1.3% (control) to 26.6 +/- 6.4% in the IL-1 exposed islets, and the ratio between labelled insulin 1 to 2 was increased from 2.0 +/- 0.1 to 3.4 +/- 0.4, respectively. Pulse-chase experiments with [3H]leucine and [35S]methionine indicated a more marked reduction in the conversion rate of proinsulin-2 compared to that of proinsulin-1. In conclusion these experiments demonstrate that IL-1 inhibits insulin biosynthesis by preferential attenuation of the rate of conversion of proinsulin 2.

AB - Insulin dependent diabetes mellitus (IDDM) is often preceded or associated with lymphocytic infiltration in the islets of Langerhans (insulitis). We recently demonstrated that interleukin-1 (IL-1) produced by activated macrophages exerts a bimodal effect on insulin release and biosynthesis in isolated rat islets. In the present study we have further analysed the effect of recombinant human interleukin-1 beta (rIL-1) on the biosynthesis and conversion of proinsulin 1 and 2 in rat islets. By RP-HPLC-analysis of islets labelled with [3H]leucine we found that exposure to 6 ng/ml of IL-1 for 24 h reduced the insulin biosynthesis to 6.1 +/- 2.7% (n = 4). During the 3 h labelling period the labelled proinsulin content compared to insulin was increased from 9.0 +/- 1.3% (control) to 26.6 +/- 6.4% in the IL-1 exposed islets, and the ratio between labelled insulin 1 to 2 was increased from 2.0 +/- 0.1 to 3.4 +/- 0.4, respectively. Pulse-chase experiments with [3H]leucine and [35S]methionine indicated a more marked reduction in the conversion rate of proinsulin-2 compared to that of proinsulin-1. In conclusion these experiments demonstrate that IL-1 inhibits insulin biosynthesis by preferential attenuation of the rate of conversion of proinsulin 2.

KW - Animals

KW - Cells, Cultured

KW - Humans

KW - Insulin

KW - Interleukin-1

KW - Islets of Langerhans

KW - Kinetics

KW - Proinsulin

KW - Rats

KW - Recombinant Proteins

M3 - Journal article

VL - 47

SP - 305

EP - 309

JO - Biomedica Biochimica Acta

JF - Biomedica Biochimica Acta

SN - 0232-766X

IS - 4-5

ER -

ID: 47974767