Effect of inhibition of CBP-coactivated β-catenin-mediated Wnt signalling in uremic rats with vascular calcifications

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Standard

Effect of inhibition of CBP-coactivated β-catenin-mediated Wnt signalling in uremic rats with vascular calcifications. / Gravesen, Eva; Nordholm, Anders; Mace, Maria; Morevati, Marya; Høgdall, Estrid; Nielsen, Carsten; Kjær, Andreas; Olgaard, Klaus; Lewin, Ewa.

I: PLOS ONE, Bind 13, Nr. 8, e0201936, 2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Gravesen, E, Nordholm, A, Mace, M, Morevati, M, Høgdall, E, Nielsen, C, Kjær, A, Olgaard, K & Lewin, E 2018, 'Effect of inhibition of CBP-coactivated β-catenin-mediated Wnt signalling in uremic rats with vascular calcifications', PLOS ONE, bind 13, nr. 8, e0201936. https://doi.org/10.1371/journal.pone.0201936

APA

Gravesen, E., Nordholm, A., Mace, M., Morevati, M., Høgdall, E., Nielsen, C., ... Lewin, E. (2018). Effect of inhibition of CBP-coactivated β-catenin-mediated Wnt signalling in uremic rats with vascular calcifications. PLOS ONE, 13(8), [e0201936]. https://doi.org/10.1371/journal.pone.0201936

Vancouver

Gravesen E, Nordholm A, Mace M, Morevati M, Høgdall E, Nielsen C o.a. Effect of inhibition of CBP-coactivated β-catenin-mediated Wnt signalling in uremic rats with vascular calcifications. PLOS ONE. 2018;13(8). e0201936. https://doi.org/10.1371/journal.pone.0201936

Author

Gravesen, Eva ; Nordholm, Anders ; Mace, Maria ; Morevati, Marya ; Høgdall, Estrid ; Nielsen, Carsten ; Kjær, Andreas ; Olgaard, Klaus ; Lewin, Ewa. / Effect of inhibition of CBP-coactivated β-catenin-mediated Wnt signalling in uremic rats with vascular calcifications. I: PLOS ONE. 2018 ; Bind 13, Nr. 8.

Bibtex

@article{f362e2b94c334b40b6584d619c9f881b,
title = "Effect of inhibition of CBP-coactivated β-catenin-mediated Wnt signalling in uremic rats with vascular calcifications",
abstract = "Uremic vascular calcification is a regulated cell-mediated process wherein cells in the arterial wall transdifferentiate to actively calcifying cells resulting in a process resembling bone formation. Wnt signalling is established as a major driver for vessel formation and maturation and for embryonic bone formation, and disturbed Wnt signalling might play a role in vascular calcification. ICG-001 is a small molecule Wnt inhibitor that specifically targets the coactivator CREB binding protein (CBP)/β-catenin-mediated signalling. In the present investigation we examined the effect of ICG-001 on vascular calcification in uremic rats. Uremic vascular calcification was induced in adult male rats by 5/6-nephrectomy, high phosphate diet and alfacalcidol. The presence of uremic vascular calcification in the aorta was associated with induction of gene expression of the Wnt target gene and marker of proliferation, cyclinD1; the mediator of canonical Wnt signalling, β-catenin and the matricellular proteins, fibronectin and periostin. Furthermore, genes from fibrosis-related pathways, TGF-β and activin A, as well as factors related to epithelial-mesenchymal transition, snail1 and vimentin were induced. ICG-001 treatment had significant effects on gene expression in kidney and aorta from healthy rats. These effects were however limited in uremic rats, and treatment with ICG-001 did not reduce the Ca-content of the uremic vasculature.",
author = "Eva Gravesen and Anders Nordholm and Maria Mace and Marya Morevati and Estrid H{\o}gdall and Carsten Nielsen and Andreas Kj{\ae}r and Klaus Olgaard and Ewa Lewin",
year = "2018",
doi = "10.1371/journal.pone.0201936",
language = "English",
volume = "13",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

RIS

TY - JOUR

T1 - Effect of inhibition of CBP-coactivated β-catenin-mediated Wnt signalling in uremic rats with vascular calcifications

AU - Gravesen, Eva

AU - Nordholm, Anders

AU - Mace, Maria

AU - Morevati, Marya

AU - Høgdall, Estrid

AU - Nielsen, Carsten

AU - Kjær, Andreas

AU - Olgaard, Klaus

AU - Lewin, Ewa

PY - 2018

Y1 - 2018

N2 - Uremic vascular calcification is a regulated cell-mediated process wherein cells in the arterial wall transdifferentiate to actively calcifying cells resulting in a process resembling bone formation. Wnt signalling is established as a major driver for vessel formation and maturation and for embryonic bone formation, and disturbed Wnt signalling might play a role in vascular calcification. ICG-001 is a small molecule Wnt inhibitor that specifically targets the coactivator CREB binding protein (CBP)/β-catenin-mediated signalling. In the present investigation we examined the effect of ICG-001 on vascular calcification in uremic rats. Uremic vascular calcification was induced in adult male rats by 5/6-nephrectomy, high phosphate diet and alfacalcidol. The presence of uremic vascular calcification in the aorta was associated with induction of gene expression of the Wnt target gene and marker of proliferation, cyclinD1; the mediator of canonical Wnt signalling, β-catenin and the matricellular proteins, fibronectin and periostin. Furthermore, genes from fibrosis-related pathways, TGF-β and activin A, as well as factors related to epithelial-mesenchymal transition, snail1 and vimentin were induced. ICG-001 treatment had significant effects on gene expression in kidney and aorta from healthy rats. These effects were however limited in uremic rats, and treatment with ICG-001 did not reduce the Ca-content of the uremic vasculature.

AB - Uremic vascular calcification is a regulated cell-mediated process wherein cells in the arterial wall transdifferentiate to actively calcifying cells resulting in a process resembling bone formation. Wnt signalling is established as a major driver for vessel formation and maturation and for embryonic bone formation, and disturbed Wnt signalling might play a role in vascular calcification. ICG-001 is a small molecule Wnt inhibitor that specifically targets the coactivator CREB binding protein (CBP)/β-catenin-mediated signalling. In the present investigation we examined the effect of ICG-001 on vascular calcification in uremic rats. Uremic vascular calcification was induced in adult male rats by 5/6-nephrectomy, high phosphate diet and alfacalcidol. The presence of uremic vascular calcification in the aorta was associated with induction of gene expression of the Wnt target gene and marker of proliferation, cyclinD1; the mediator of canonical Wnt signalling, β-catenin and the matricellular proteins, fibronectin and periostin. Furthermore, genes from fibrosis-related pathways, TGF-β and activin A, as well as factors related to epithelial-mesenchymal transition, snail1 and vimentin were induced. ICG-001 treatment had significant effects on gene expression in kidney and aorta from healthy rats. These effects were however limited in uremic rats, and treatment with ICG-001 did not reduce the Ca-content of the uremic vasculature.

U2 - 10.1371/journal.pone.0201936

DO - 10.1371/journal.pone.0201936

M3 - Journal article

C2 - 30075015

AN - SCOPUS:85052377205

VL - 13

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 8

M1 - e0201936

ER -

ID: 214761516