Effect of apolipoprotein M on high density lipoprotein metabolism and atherosclerosis in low density lipoprotein receptor knock-out mice

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Effect of apolipoprotein M on high density lipoprotein metabolism and atherosclerosis in low density lipoprotein receptor knock-out mice. / Christoffersen, Christina; Jauhiainen, Matti; Moser, Markus; Porse, Bo; Ehnholm, Christian; Boesl, Michael; Dahlbäck, Björn; Nielsen, Lars Bo.

I: The Journal of Biological Chemistry, Bind 283, Nr. 4, 25.01.2008, s. 1839-47.

Publikation: Bidrag til tidsskriftTidsskriftartikel

Harvard

Christoffersen, C, Jauhiainen, M, Moser, M, Porse, B, Ehnholm, C, Boesl, M, Dahlbäck, B & Nielsen, LB 2008, 'Effect of apolipoprotein M on high density lipoprotein metabolism and atherosclerosis in low density lipoprotein receptor knock-out mice', The Journal of Biological Chemistry, bind 283, nr. 4, s. 1839-47. https://doi.org/10.1074/jbc.M704576200

APA

Christoffersen, C., Jauhiainen, M., Moser, M., Porse, B., Ehnholm, C., Boesl, M., Dahlbäck, B., & Nielsen, L. B. (2008). Effect of apolipoprotein M on high density lipoprotein metabolism and atherosclerosis in low density lipoprotein receptor knock-out mice. The Journal of Biological Chemistry, 283(4), 1839-47. https://doi.org/10.1074/jbc.M704576200

Vancouver

Christoffersen C, Jauhiainen M, Moser M, Porse B, Ehnholm C, Boesl M o.a. Effect of apolipoprotein M on high density lipoprotein metabolism and atherosclerosis in low density lipoprotein receptor knock-out mice. The Journal of Biological Chemistry. 2008 jan. 25;283(4):1839-47. https://doi.org/10.1074/jbc.M704576200

Author

Christoffersen, Christina ; Jauhiainen, Matti ; Moser, Markus ; Porse, Bo ; Ehnholm, Christian ; Boesl, Michael ; Dahlbäck, Björn ; Nielsen, Lars Bo. / Effect of apolipoprotein M on high density lipoprotein metabolism and atherosclerosis in low density lipoprotein receptor knock-out mice. I: The Journal of Biological Chemistry. 2008 ; Bind 283, Nr. 4. s. 1839-47.

Bibtex

@article{e8589c3fec5a4b7d9c2a9d29b735af05,
title = "Effect of apolipoprotein M on high density lipoprotein metabolism and atherosclerosis in low density lipoprotein receptor knock-out mice",
abstract = "To investigate the role of apoM in high density lipoprotein (HDL) metabolism and atherogenesis, we generated human apoM transgenic (apoM-Tg) and apoM-deficient (apoM(-/-)) mice. Plasma apoM was predominantly associated with 10-12-nm alpha-migrating HDL particles. Human apoM overexpression (11-fold) increased plasma cholesterol concentration by 13-22%, whereas apoM deficiency decreased it by 17-21%. The size and charge of apoA-I-containing HDL in plasma were not changed in apoM-Tg or apoM(-/-) mice. However, in plasma incubated at 37 degrees C, lecithin:cholesterol acyltransferase-dependent conversion of alpha- to pre-alpha-migrating HDL was delayed in apoM-Tg mice. Moreover, lecithin: cholesterol acyltransferase-independent generation of pre-beta-migrating apoA-I-containing particles in plasma was increased in apoM-Tg mice (4.2 +/- 1.1%, p = 0.06) and decreased in apoM(-/-) mice (0.5 +/- 0.3%, p = 0.03) versus controls (1.8 +/- 0.05%). In the setting of low density lipoprotein receptor deficiency, apoM-Tg mice with approximately 2-fold increased plasma apoM concentrations developed smaller atherosclerotic lesions than controls. The effect of apoM on atherosclerosis may be facilitated by enzymatic modulation of plasma HDL particles, increased cholesterol efflux from foam cells, and an antioxidative effect of apoM-containing HDL.",
keywords = "Animals, Antioxidants, Apolipoprotein A-I, Apolipoproteins, Atherosclerosis, Cholesterol, Foam Cells, Gene Expression, Humans, Lipocalins, Lipoproteins, HDL, Mice, Mice, Knockout, Multiprotein Complexes, Phosphatidylcholine-Sterol O-Acyltransferase, Receptors, LDL",
author = "Christina Christoffersen and Matti Jauhiainen and Markus Moser and Bo Porse and Christian Ehnholm and Michael Boesl and Bj{\"o}rn Dahlb{\"a}ck and Nielsen, {Lars Bo}",
year = "2008",
month = jan,
day = "25",
doi = "10.1074/jbc.M704576200",
language = "English",
volume = "283",
pages = "1839--47",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - Effect of apolipoprotein M on high density lipoprotein metabolism and atherosclerosis in low density lipoprotein receptor knock-out mice

AU - Christoffersen, Christina

AU - Jauhiainen, Matti

AU - Moser, Markus

AU - Porse, Bo

AU - Ehnholm, Christian

AU - Boesl, Michael

AU - Dahlbäck, Björn

AU - Nielsen, Lars Bo

PY - 2008/1/25

Y1 - 2008/1/25

N2 - To investigate the role of apoM in high density lipoprotein (HDL) metabolism and atherogenesis, we generated human apoM transgenic (apoM-Tg) and apoM-deficient (apoM(-/-)) mice. Plasma apoM was predominantly associated with 10-12-nm alpha-migrating HDL particles. Human apoM overexpression (11-fold) increased plasma cholesterol concentration by 13-22%, whereas apoM deficiency decreased it by 17-21%. The size and charge of apoA-I-containing HDL in plasma were not changed in apoM-Tg or apoM(-/-) mice. However, in plasma incubated at 37 degrees C, lecithin:cholesterol acyltransferase-dependent conversion of alpha- to pre-alpha-migrating HDL was delayed in apoM-Tg mice. Moreover, lecithin: cholesterol acyltransferase-independent generation of pre-beta-migrating apoA-I-containing particles in plasma was increased in apoM-Tg mice (4.2 +/- 1.1%, p = 0.06) and decreased in apoM(-/-) mice (0.5 +/- 0.3%, p = 0.03) versus controls (1.8 +/- 0.05%). In the setting of low density lipoprotein receptor deficiency, apoM-Tg mice with approximately 2-fold increased plasma apoM concentrations developed smaller atherosclerotic lesions than controls. The effect of apoM on atherosclerosis may be facilitated by enzymatic modulation of plasma HDL particles, increased cholesterol efflux from foam cells, and an antioxidative effect of apoM-containing HDL.

AB - To investigate the role of apoM in high density lipoprotein (HDL) metabolism and atherogenesis, we generated human apoM transgenic (apoM-Tg) and apoM-deficient (apoM(-/-)) mice. Plasma apoM was predominantly associated with 10-12-nm alpha-migrating HDL particles. Human apoM overexpression (11-fold) increased plasma cholesterol concentration by 13-22%, whereas apoM deficiency decreased it by 17-21%. The size and charge of apoA-I-containing HDL in plasma were not changed in apoM-Tg or apoM(-/-) mice. However, in plasma incubated at 37 degrees C, lecithin:cholesterol acyltransferase-dependent conversion of alpha- to pre-alpha-migrating HDL was delayed in apoM-Tg mice. Moreover, lecithin: cholesterol acyltransferase-independent generation of pre-beta-migrating apoA-I-containing particles in plasma was increased in apoM-Tg mice (4.2 +/- 1.1%, p = 0.06) and decreased in apoM(-/-) mice (0.5 +/- 0.3%, p = 0.03) versus controls (1.8 +/- 0.05%). In the setting of low density lipoprotein receptor deficiency, apoM-Tg mice with approximately 2-fold increased plasma apoM concentrations developed smaller atherosclerotic lesions than controls. The effect of apoM on atherosclerosis may be facilitated by enzymatic modulation of plasma HDL particles, increased cholesterol efflux from foam cells, and an antioxidative effect of apoM-containing HDL.

KW - Animals

KW - Antioxidants

KW - Apolipoprotein A-I

KW - Apolipoproteins

KW - Atherosclerosis

KW - Cholesterol

KW - Foam Cells

KW - Gene Expression

KW - Humans

KW - Lipocalins

KW - Lipoproteins, HDL

KW - Mice

KW - Mice, Knockout

KW - Multiprotein Complexes

KW - Phosphatidylcholine-Sterol O-Acyltransferase

KW - Receptors, LDL

U2 - 10.1074/jbc.M704576200

DO - 10.1074/jbc.M704576200

M3 - Journal article

C2 - 18006500

VL - 283

SP - 1839

EP - 1847

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 4

ER -

ID: 100888385